Scholars@Duke
Jamie Rae Privratsky

Jamie Rae Privratsky

Associate Professorship in Anesthesiology
Anesthesiology, Critical Care Medicine
jamie.privratsky@duke.edu
DUMC Box 3094, Durham, NC 27710
303 Research Dr, Alexander Sands Bldg, Room 101, Durham, NC 27710

Selected Publications

Association of Causative Pathogens With Acute Kidney Injury in Adult Patients With Community-Onset Sepsis.

Journal Article Crit Care Explor · February 1, 2025 IMPORTANCE: The influence of disease-causing pathogen on acute kidney injury (AKI) in septic patients is poorly understood. OBJECTIVES: We examined the association of microbial pathogen with AKI among patients with community-onset sepsis. DESIGN, SETTING, ... Full text Link to item Cite

Targeting allograft inflammatory factor 1 reprograms kidney macrophages to enhance repair.

Journal Article J Clin Invest · January 21, 2025 The role of macrophages (MΦs) remains incompletely understood in kidney injury and repair. The plasticity of MΦs offers an opportunity to polarize them toward mediating injury resolution in both native and transplanted kidneys undergoing ischemia and/or re ... Full text Link to item Cite

Murine kidney transplant outcome is best measured by transdermal glomerular filtration rate.

Journal Article Am J Transplant · December 2024 Mouse kidney transplantation provides a powerful preclinical model for the study of kidney transplant alloimmunity. However, accurate measurement of graft function is difficult because of the inaccuracy of traditional surrogate markers serum creatinine and ... Full text Link to item Cite

Kidney Macrophage IL-1 Receptor Limits Endoplasmic Reticulum Stress and Nephrotoxic Serum Nephritis

Conference Journal of the American Society of Nephrology · October 2024 Full text Cite

Metallothionein Alleviates Glutathione Depletion-Induced Oxidative Cardiomyopathy through CISD1-Dependent Regulation of Ferroptosis in Murine Hearts.

Journal Article Am J Pathol · June 2024 This study was designed to discern the effect of heavy scavenger metallothionein on glutathione (GSH) deprivation-evoked cardiac anomalies and mechanisms involved with an emphasis on ferroptosis. Wild-type and cardiac metallothionein transgenic mice receiv ... Full text Link to item Cite

Blood or Fat? Differentiating Hemopericardium versus Epicardial Fat Using Focused Cardiac Ultrasound.

Journal Article Diagnostics (Basel) · April 15, 2024 Basic point-of-care ultrasound of the heart-also known as Focused Cardiac Ultrasound (FoCUS)-has emerged as a powerful bedside tool to narrow the differential diagnosis of causes of hypotension. The list of causes of hypotension that a FoCUS provider is ex ... Full text Link to item Cite

TNF- α from the Proximal Nephron Exacerbates Aristolochic Acid Nephropathy.

Journal Article Kidney360 · January 1, 2024 KEY POINTS: Proximal tubular TNF aggravates kidney injury and fibrogenesis in aristolochic acid nephropathy. Tubular TNF disrupts the cell cycle in injured tubular epithelial cells. TNF-mediated toxic renal injury is independent of systemic immune response ... Full text Link to item Cite

Novel anti-inflammatory effects of the IL-1 receptor in kidney myeloid cells following ischemic AKI.

Journal Article Front Mol Biosci · 2024 Introduction: Acute kidney injury (AKI) is one of the most common causes of organ failure in critically ill patients. Following AKI, the canonical pro-inflammatory cytokine interleukin-1β (IL-1β) is released predominantly from activated myeloid cells and b ... Full text Link to item Cite

Divergent Actions of Renal Tubular and Endothelial Type 1 IL-1 Receptor Signaling in Toxin-Induced AKI.

Journal Article J Am Soc Nephrol · October 1, 2023 SIGNIFICANCE STATEMENT: Activation of the type 1 IL-1 receptor (IL-1R1) triggers a critical innate immune signaling cascade that contributes to the pathogenesis of AKI. However, blockade of IL-1 signaling in AKI has not consistently demonstrated kidney pro ... Full text Link to item Cite

A macrophage-endothelial immunoregulatory axis ameliorates septic acute kidney injury.

Journal Article Kidney Int · March 2023 The most common cause of acute kidney injury (AKI) in critically ill patients is sepsis. Kidney macrophages consist of both F4/80hi and CD11bhi cells. The role of macrophage subpopulations in septic AKI pathogenesis remains unclear. As F4/80hi macrophages ... Full text Link to item Cite

Postoperative Acute Kidney Injury by Age and Sex: A Retrospective Cohort Association Study.

Journal Article Anesthesiology · February 1, 2023 BACKGROUND: Acute kidney injury (AKI) after noncardiac surgery is common and has substantial health impact. Preclinical and clinical studies examining the influence of sex on AKI have yielded conflicting results, although they typically do not account for ... Full text Link to item Cite

Novel Anti-Inflammatory Effects of IL-1 Receptor in Kidney Myeloid Cells Following AKI

Conference JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · 2023 Cite

Identification of Trajectory-Based Acute Kidney Injury Phenotypes Among Cardiac Surgery Patients.

Journal Article Ann Thorac Surg · December 2022 BACKGROUND: Acute kidney injury (AKI) is a common and serious complication of cardiac surgical procedures for which unrecognized heterogeneity may underpin poor success in identifying effective therapies. We aimed to identify phenotypically similar groups ... Full text Link to item Cite

Angiotensin II enhances bacterial clearance via myeloid signaling in a murine sepsis model.

Journal Article Proc Natl Acad Sci U S A · August 23, 2022 Sepsis, defined as organ dysfunction caused by a dysregulated host-response to infection, is characterized by immunosuppression. The vasopressor norepinephrine is widely used to treat low blood pressure in sepsis but exacerbates immunosuppression. An alter ... Full text Link to item Cite

IL-1 receptor signaling in podocytes limits susceptibility to glomerular damage.

Journal Article Am J Physiol Renal Physiol · February 1, 2022 Interleukin (IL)-1 receptor type 1 (IL-1R1) activation triggers a proinflammatory signaling cascade that can exacerbate kidney injury. However, the functions of podocyte IL-1R1 in glomerular disease remain unclear. To study the role of IL-1R1 signaling in ... Full text Link to item Cite

Postoperative Acute Kidney Injury Is Associated With Progression of Chronic Kidney Disease Independent of Severity.

Journal Article Anesth Analg · January 1, 2022 BACKGROUND: Both postoperative acute kidney injury (AKI) and preoperative chronic kidney disease (CKD) are associated with significantly worse outcomes following surgery. The relationship of both of these conditions with each other and with CKD progression ... Full text Link to item Cite

Association of Severe Acute Kidney Injury with Mortality and Healthcare Utilization Following Isolated Traumatic Brain Injury.

Journal Article Neurocrit Care · October 2021 BACKGROUND/OBJECTIVE: Traumatic brain injury (TBI) is a leading cause of morbidity, mortality, and disability in the USA. While cardiopulmonary dysfunction can result in poor outcomes following severe TBI, the impact of acute kidney injury (AKI) is poorly ... Full text Open Access Link to item Cite

Twist1 in podocytes ameliorates podocyte injury and proteinuria by limiting CCL2-dependent macrophage infiltration.

Journal Article JCI Insight · August 9, 2021 The transcription factor Twist1 regulates several processes that could impact kidney disease progression, including epithelial cell differentiation and inflammatory cytokine induction. Podocytes are specialized epithelia that exhibit features of immune cel ... Full text Link to item Cite

Twist1 in T Lymphocytes Augments Kidney Fibrosis after Ureteral Obstruction.

Journal Article Kidney360 · May 27, 2021 BACKGROUND: Twist1 is a basic helix-loop-helix domain-containing transcription factor that participates in diverse cellular functions, including epithelial-mesenchymal transition and the cellular immune response. Although Twist1 plays critical roles in the ... Full text Link to item Cite

In reply to: "Intra-aortic balloon pump protects against hydrostatic pulmonary oedema during peripheral venoarterial-extracorporeal membrane oxygenation".

Journal Article Eur Heart J Acute Cardiovasc Care · March 5, 2021 Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) is an increasingly utilized life-saving measure. However, left-ventricular distention from inadequate left-ventricular off-loading can lead to unwanted pulmonary and cardiac complications. We are ... Full text Link to item Cite

Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes.

Conference Front Immunol · 2021 Intestinal immunity is coordinated by specialized mononuclear phagocyte populations, constituted by a diversity of cell subsets. Although the cell subsets constituting the mononuclear phagocyte network are thought to be similar in both small and large inte ... Full text Link to item Cite

Annexin A1 Tripeptide Mimetic Increases Sirtuin-3 and Augments Mitochondrial Function to Limit Ischemic Kidney Injury.

Journal Article Front Physiol · 2021 Background: Acute kidney injury (AKI) is one of the most common organ failures following surgery. We have developed a tripeptide mimetic (ANXA1sp) of the parent annexin A1 molecule that shows promise as an organ protectant limiting cellular stress; however ... Full text Link to item Cite

Preservation of Renal Function

Chapter · January 1, 2021 Acute kidney injury (AKI) remains a frequent and serious complication of surgical procedures and critical illness that is consistently associated with worse outcomes and increased long-term morbidity and mortality. Much work has gone into finding kidney pr ... Full text Cite

Classical Dendritic Cells Mediate Nephrotoxic Serum Nephritis by Activating T Cells

Conference JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · 2021 Cite

Resident Macrophages Limit IL-6 generation to Protect Against Septic AKI

Conference JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · 2021 Cite

Apolipoprotein L1 (APOL1) Coding Variants Are Associated With Creatinine Rise After Cardiac Surgery.

Journal Article J Cardiothorac Vasc Anesth · December 2020 OBJECTIVE: Acute kidney injury (AKI) is a complication of cardiac surgery that is considerably more common in African Americans (1.5-fold). Although homozygous status for apolipoprotein L1 (APOL1) risk alleles is associated with chronic kidney disease in i ... Full text Link to item Cite

Intraoperative renal resistive index threshold as an acute kidney injury biomarker.

Journal Article J Clin Anesth · May 2020 STUDY OBJECTIVE: The lag in creatinine-mediated diagnosis of cardiac surgery-associated acute kidney injury (AKI) may be impeding the development of renoprotection therapies. Postoperative renal resistive index (RRI) measured by transabdominal Doppler ultr ... Full text Link to item Cite

Yolk-sac-derived macrophages progressively expand in the mouse kidney with age.

Journal Article Elife · April 17, 2020 Renal macrophages represent a highly heterogeneous and specialized population of myeloid cells with mixed developmental origins from the yolk-sac and hematopoietic stem cells (HSC). They promote both injury and repair by regulating inflammation, angiogenes ... Full text Link to item Cite

C-C Motif Chemokine Receptor 7 Exacerbates Hypertension Through Effects on T Lymphocyte Trafficking.

Journal Article Hypertension · March 2020 Activated T lymphocytes that infiltrate blood pressure control organs make a critical contribution to the pathogenesis of hypertension. Dendritic cells act as potent antigen-presenting cells to stimulate prohypertensive T cells. However, the mechanisms tha ... Full text Link to item Cite

The transcription factor Twist1 in the distal nephron but not in macrophages propagates aristolochic acid nephropathy.

Journal Article Kidney Int · January 2020 Tubulointerstitial disease in the kidney culminates in renal fibrosis that portents organ failure. Twist1, a basic helix-loop-helix protein 38 transcription factor, regulates several essential biological functions, but inappropriate Twist1 activity in the ... Full text Link to item Cite

TNF-α in T lymphocytes attenuates renal injury and fibrosis during nephrotoxic nephritis.

Journal Article Am J Physiol Renal Physiol · January 1, 2020 Nephrotoxic serum nephritis (NTN) models immune-mediated human glomerulonephritis and culminates in kidney inflammation and fibrosis, a process regulated by T lymphocytes. TNF-α is a key proinflammatory cytokine that contributes to diverse forms of renal i ... Full text Link to item Cite

Classical Dendritic Cells Mediate Hypertension by Promoting Renal Oxidative Stress and Fluid Retention.

Journal Article Hypertension · January 2020 FLT3L (Fms-like tyrosine kinase 3 ligand) stimulates the development of classical dendritic cells (DCs). Here we tested the hypothesis that classical DCs drive blood pressure elevation by promoting renal fluid retention. FLT3L-deficient (FLT3L-/-) mice tha ... Full text Link to item Cite

Renal Failure and Dialysis

Chapter · January 1, 2020 Acute kidney injury (AKI) is a common and serious complication of cardiac surgery. The spectrum of cardiac surgery-associated AKI (CS-AKI) ranges from transient loss of renal reserve, through AKI, to long-term irreversible renal failure requiring renal rep ... Full text Cite

A20 in Myeloid Cells Protects Against Hypertension by Inhibiting Dendritic Cell-Mediated T-Cell Activation.

Journal Article Circ Res · December 6, 2019 RATIONALE: The ubiquitin-editing protein A20 in dendritic cells (DCs) suppresses NF-κB (nuclear factor-κB) signaling and constrains DC-mediated T-cell stimulation, but the role of A20 in modulating the hypertensive response requires elucidation. OBJECTIVE: ... Full text Link to item Cite

Opposing actions of renal tubular- and myeloid-derived porcupine in obstruction-induced kidney fibrosis.

Journal Article Kidney Int · December 2019 Wnt/β-catenin signaling is essential in the pathogenesis of renal fibrosis. We previously reported inhibition of the Wnt O-acyl transferase porcupine, required for Wnt secretion, dramatically attenuates kidney fibrosis in the murine unilateral ureteral obs ... Full text Link to item Cite

KLF4 in Macrophages Attenuates TNFα-Mediated Kidney Injury and Fibrosis.

Journal Article J Am Soc Nephrol · October 2019 BACKGROUND: Polarized macrophage populations can orchestrate both inflammation of the kidney and tissue repair during CKD. Proinflammatory M1 macrophages initiate kidney injury, but mechanisms through which persistent M1-dependent kidney damage culminates ... Full text Link to item Cite

Twist1 in Infiltrating Macrophages Attenuates Kidney Fibrosis via Matrix Metallopeptidase 13-Mediated Matrix Degradation.

Journal Article J Am Soc Nephrol · September 2019 BACKGROUND: Following an acute insult, macrophages regulate renal fibrogenesis through the release of various factors that either encourage the synthesis of extracellular matrix synthesis or the degradation of matrix via endocytosis, proteolysis, or both. ... Full text Link to item Cite

Interleukin-1 receptor activation aggravates autosomal dominant polycystic kidney disease by modulating regulated necrosis.

Journal Article Am J Physiol Renal Physiol · August 1, 2019 Autosomal dominant polycystic kidney disease (ADPKD) is associated with increased chemokines, cytokines, and growth factors in the diseased kidney. We found that both isoforms of IL-1, IL-1α and IL-1β, were upregulated in ADPKD tissues. Here, we used a uni ... Full text Link to item Cite

Stimulating Type 1 Angiotensin Receptors on T Lymphocytes Attenuates Renal Fibrosis.

Journal Article Am J Pathol · May 2019 Most forms of chronic kidney disease culminate in renal fibrosis that heralds organ failure. In contrast to the protective effects of globally blocking type 1 angiotensin (AT1) receptors throughout the body, activating AT1 receptors directly on immune cell ... Full text Link to item Cite

Corrigendum

Journal Article American Journal of Physiology-Renal Physiology · May 1, 2019 Full text Cite

Dynamic contrast-enhanced MRI promotes early detection of toxin-induced acute kidney injury.

Journal Article Am J Physiol Renal Physiol · February 1, 2019 Acute kidney injury (AKI) is a common cause of morbidity and mortality in hospitalized patients. Nevertheless, there is limited ability to diagnose AKI in its earliest stages through the collection of structural and functional information. Magnetic resonan ... Full text Link to item Cite

Interleukin 1 receptor (IL-1R1) activation exacerbates toxin-induced acute kidney injury.

Journal Article Am J Physiol Renal Physiol · September 1, 2018 Acute kidney injury (AKI) is a leading cause of morbidity and mortality. Drug-induced/toxic AKI can be caused by a number of therapeutic agents. Cisplatin is an effective chemotherapeutic agent whose administration is limited by significant nephrotoxicity. ... Full text Link to item Cite

Competing Actions of Type 1 Angiotensin II Receptors Expressed on T Lymphocytes and Kidney Epithelium during Cisplatin-Induced AKI.

Journal Article J Am Soc Nephrol · August 2016 Inappropriate activation of the renin-angiotensin system (RAS) contributes to many CKDs. However, the role of the RAS in modulating AKI requires elucidation, particularly because stimulating type 1 angiotensin II (AT1) receptors in the kidney or circulatin ... Full text Open Access Link to item Cite

PECAM-1: regulator of endothelial junctional integrity.

Journal Article Cell Tissue Res · March 2014 PECAM-1 (also known as CD31) is a cellular adhesion and signaling receptor comprising six extracellular immunoglobulin (Ig)-like homology domains, a short transmembrane domain and a 118 amino acid cytoplasmic domain that becomes serine and tyrosine phospho ... Full text Link to item Cite

PECAM-1 dampens cytokine levels during LPS-induced endotoxemia by regulating leukocyte trafficking.

Journal Article Life Sci · January 30, 2012 AIMS: To investigate the mechanism by which platelet endothelial cell adhesion molecule 1 (PECAM-1/CD31), an immunoglobulin (Ig)-superfamily cell adhesion and signaling receptor, regulates pro-inflammatory cytokine levels. The purpose of the present invest ... Full text Link to item Cite

Outcomes of grafted bulbar urethroplasty in men with class II or III obesity.

Journal Article Urology · December 2011 OBJECTIVE: To evaluate the outcomes of grafted bulbar urethroplasty in class II or III obese patients. MATERIAL AND METHODS: Retrospective chart review was performed for men with World Health Organization class II/III obesity undergoing grafted urethroplas ... Full text Link to item Cite

Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity.

Journal Article J Cell Sci · May 1, 2011 PECAM-1 (CD31) is a cellular adhesion and signaling receptor that is highly expressed at endothelial cell-cell junctions in confluent vascular beds. Previous studies have implicated PECAM-1 in the maintenance of vascular barrier integrity; however, the mec ... Full text Link to item Cite

Relative Contribution of PECAM-1 Adhesion and Signaling to the Maintenance of Vascular Integrity

Conference ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY · November 1, 2010 Link to item Cite

The Anti-inflammatory Actions of PECAM-1 Do Not Involve Regulation of Endothelial Cell NF kappa B

Conference ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY · November 1, 2010 Link to item Cite

PECAM-1: conflicts of interest in inflammation.

Journal Article Life Sci · July 17, 2010 Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a cell adhesion and signaling receptor that is expressed on hematopoietic and endothelial cells. PECAM-1 is vital to the regulation of inflammatory responses, as it has been shown to serve a ... Full text Link to item Cite

The anti-inflammatory actions of platelet endothelial cell adhesion molecule-1 do not involve regulation of endothelial cell NF-kappa B.

Journal Article J Immunol · March 15, 2010 PECAM-1 is a cell adhesion and signaling receptor that is expressed on many hematopoietic cells and at endothelial cell-cell junctions. Accumulating evidence from a number of in vitro and in vivo model systems suggests that PECAM-1 suppresses cytokine prod ... Full text Link to item Cite

Metallothionein abrogates GTP cyclohydrolase I inhibition-induced cardiac contractile and morphological defects: role of mitochondrial biogenesis.

Journal Article Hypertension · June 2009 One key mechanism for endothelial dysfunction is endothelial NO synthase (eNOS) uncoupling, whereby eNOS generates O(2)(*-) rather than NO because of deficient eNOS cofactor tetrahydrobiopterin (BH4). This study was designed to examine the effect of BH4 de ... Full text Link to item Cite

Metallothionein alleviates glutathione depletion-induced oxidative cardiomyopathy in murine hearts.

Journal Article Crit Care Med · July 2008 OBJECTIVE: Antioxidant therapy has shown some promise in critical care medicine in which glutathione depletion and heart failure are often seen in critically ill patients. This study was designed to examine the impact of glutathione depletion and the free ... Full text Link to item Cite

Metallothionein alleviates cardiac dysfunction in streptozotocin-induced diabetes: role of Ca2+ cycling proteins, NADPH oxidase, poly(ADP-Ribose) polymerase and myosin heavy chain isozyme.

Journal Article Free Radic Biol Med · April 15, 2006 Diabetic cardiomyopathy contributes to high morbidity and mortality in diabetic populations. It is manifested by compromised ventricular contraction and prolonged relaxation attributable to multiple causative factors including oxidative stress. This study ... Full text Link to item Cite

Inhibition of sarco(endo)plasmic reticulum Ca2+-ATPase differentially regulates contractile function in cardiac myocytes from normotensive and spontaneously hypertensive rats: role of Ca2+ regulatory proteins.

Journal Article Cell Biochem Biophys · 2005 Hypertension leads to impaired contractile function. This study examined the impact of inhibition of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by thapsigargin or cyclopiazonic acid (CPA) on cardiac contractile function in ventricular myocytes from W ... Full text Link to item Cite

AT1 blockade prevents glucose-induced cardiac dysfunction in ventricular myocytes: role of the AT1 receptor and NADPH oxidase.

Journal Article Hypertension · August 2003 Enhanced tissue angiotensin (Ang) II levels have been reported in diabetes and might lead to cardiac dysfunction through oxidative stress. This study examined the effect of blocking the Ang II type 1 (AT1) receptor on high glucose-induced cardiac contracti ... Full text Link to item Cite

Combined acetaldehyde and nicotine exposure depresses cardiac contraction in ventricular myocytes: prevention by folic acid.

Journal Article Neurotoxicol Teratol · 2003 Chronic alcoholism can progress to alcoholic cardiomyopathy characterized by ventricular dilation and impaired ventricular contractility. Nicotine abuse continues to remain a serious risk factor for cardiovascular diseases. However, little is know regardin ... Full text Link to item Cite