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Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes.

Publication ,  Conference
Huang, H-I; Jewell, ML; Youssef, N; Huang, M-N; Hauser, ER; Fee, BE; Rudemiller, NP; Privratsky, JR; Zhang, JJ; Reyes, EY; Wang, D; Taylor, GA ...
Published in: Front Immunol
2021

Intestinal immunity is coordinated by specialized mononuclear phagocyte populations, constituted by a diversity of cell subsets. Although the cell subsets constituting the mononuclear phagocyte network are thought to be similar in both small and large intestine, these organs have distinct anatomy, microbial composition, and immunological demands. Whether these distinctions demand organ-specific mononuclear phagocyte populations with dedicated organ-specific roles in immunity are unknown. Here we implement a new strategy to subset murine intestinal mononuclear phagocytes and identify two novel subsets which are colon-specific: a macrophage subset and a Th17-inducing dendritic cell (DC) subset. Colon-specific DCs and macrophages co-expressed CD24 and CD14, and surprisingly, both were dependent on the transcription factor IRF4. Novel IRF4-dependent CD14+CD24+ macrophages were markedly distinct from conventional macrophages and failed to express classical markers including CX3CR1, CD64 and CD88, and surprisingly expressed little IL-10, which was otherwise robustly expressed by all other intestinal macrophages. We further found that colon-specific CD14+CD24+ mononuclear phagocytes were essential for Th17 immunity in the colon, and provide definitive evidence that colon and small intestine have distinct antigen presenting cell requirements for Th17 immunity. Our findings reveal unappreciated organ-specific diversity of intestine-resident mononuclear phagocytes and organ-specific requirements for Th17 immunity.

Duke Scholars

Published In

Front Immunol

DOI

EISSN

1664-3224

Publication Date

2021

Volume

12

Start / End Page

661290

Location

Switzerland

Related Subject Headings

  • Th17 Cells
  • Receptor, Anaphylatoxin C5a
  • Phagocytes
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, 129 Strain
  • Mice
  • Macrophages
  • Lipopolysaccharide Receptors
  • Intestine, Small
 

Citation

APA
Chicago
ICMJE
MLA
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Huang, H.-I., Jewell, M. L., Youssef, N., Huang, M.-N., Hauser, E. R., Fee, B. E., … Hammer, G. E. (2021). Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes. In Front Immunol (Vol. 12, p. 661290). Switzerland. https://doi.org/10.3389/fimmu.2021.661290
Huang, Hsin-I, Mark L. Jewell, Nourhan Youssef, Min-Nung Huang, Elizabeth R. Hauser, Brian E. Fee, Nathan P. Rudemiller, et al. “Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes.” In Front Immunol, 12:661290, 2021. https://doi.org/10.3389/fimmu.2021.661290.
Huang H-I, Jewell ML, Youssef N, Huang M-N, Hauser ER, Fee BE, et al. Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes. In: Front Immunol. 2021. p. 661290.
Huang, Hsin-I., et al. “Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes.Front Immunol, vol. 12, 2021, p. 661290. Pubmed, doi:10.3389/fimmu.2021.661290.
Huang H-I, Jewell ML, Youssef N, Huang M-N, Hauser ER, Fee BE, Rudemiller NP, Privratsky JR, Zhang JJ, Reyes EY, Wang D, Taylor GA, Gunn MD, Ko DC, Cook DN, Chandramohan V, Crowley SD, Hammer GE. Th17 Immunity in the Colon Is Controlled by Two Novel Subsets of Colon-Specific Mononuclear Phagocytes. Front Immunol. 2021. p. 661290.

Published In

Front Immunol

DOI

EISSN

1664-3224

Publication Date

2021

Volume

12

Start / End Page

661290

Location

Switzerland

Related Subject Headings

  • Th17 Cells
  • Receptor, Anaphylatoxin C5a
  • Phagocytes
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, 129 Strain
  • Mice
  • Macrophages
  • Lipopolysaccharide Receptors
  • Intestine, Small