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Harvey Edwin Marshall III

Professor of Medicine
Medicine, Pulmonary, Allergy, and Critical Care Medicine
Duke Box 2613, Durham, NC 27710
201MED Sci Res Bldg, Durham, NC 27710

Selected Publications


ERK-dependent proteasome degradation of Txnip regulates thioredoxin oxidoreductase activity.

Journal Article J Biol Chem · September 6, 2019 Dynamic control of thioredoxin (Trx) oxidoreductase activity is essential for balancing the need of cells to rapidly respond to oxidative/nitrosative stress and to temporally regulate thiol-based redox signaling. We have previously shown that cytokine stim ... Full text Link to item Cite

Thioredoxin-mediated denitrosylation regulates cytokine-induced nuclear factor κB (NF-κB) activation.

Journal Article J Biol Chem · January 31, 2014 S-nitrosylation of nuclear factor κB (NF-κB) on the p65 subunit of the p50/p65 heterodimer inhibits NF-κB DNA binding activity. We have recently shown that p65 is constitutively S-nitrosylated in the lung and that LPS-induced injury elicits a decrease in S ... Full text Link to item Cite

Proteomic analysis of the NOS2 interactome in human airway epithelial cells.

Journal Article Nitric Oxide · November 1, 2013 The cytokine-inducible isoform of nitric oxide synthase (NOS2) is constitutively expressed in human respiratory epithelia and is upregulated in inflammatory lung disease. Here, we sought to better define the protein interactions that may be important for N ... Full text Link to item Cite

Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.

Journal Article J Proteome Res · May 3, 2013 The analysis of airway fluid, as sampled by bronchoalveolar lavage (BAL), provides a minimally invasive route to interrogate lung biology in health and disease. Here, we used immunodepletion, coupled with gel- and label-free LC-MS/MS, for quantitation of t ... Full text Link to item Cite

Proteomic Analysis Of Human Bronchoalveolar Lavage Fluid After Subsegmental Challenge

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · 2013 Cite

S-nitrosylation of Ras in breast cancer.

Journal Article Breast Cancer Res · November 12, 2012 Elevated expression of nitric oxide synthase 2 has been recently shown to correlate with poor survival in estrogen receptor-negative breast cancer. In an article in Breast Cancer Research, Switzer and colleagues identify the transcription factor Ets-1 as a ... Full text Link to item Cite

S-nitrosylation in the regulation of gene transcription.

Journal Article Biochim Biophys Acta · June 2012 BACKGROUND: Post-translational modification of proteins by S-nitrosylation serves as a major mode of signaling in mammalian cells and a growing body of evidence has shown that transcription factors and their activating pathways are primary targets. S-nitro ... Full text Link to item Cite

The clinical and environmental determinants of airway transcriptional profiles in allergic asthma.

Journal Article Am J Respir Crit Care Med · March 15, 2012 RATIONALE: Gene expression profiling of airway epithelial and inflammatory cells can be used to identify genes involved in environmental asthma. METHODS: Airway epithelia and inflammatory cells were obtained via bronchial brush and bronchoalveolar lavage ( ... Full text Link to item Cite

NOS2 regulation of LPS-induced airway inflammation via S-nitrosylation of NF-{kappa}B p65.

Journal Article Am J Physiol Lung Cell Mol Physiol · September 2011 Inducible nitric oxide synthase (NOS2) expression is increased in the airway epithelium in acute inflammatory disorders although the physiological impact remains unclear. We have previously shown that NOS2 inhibits NF-κB (p50-p65) activation in respiratory ... Full text Link to item Cite

Quantitative Proteomics Of The Cytokine-Modulated Nos2 Interactome In Airway Epithelium

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2011 Link to item Cite

Thioredoxin-interacting protein (Txnip) is a feedback regulator of S-nitrosylation.

Journal Article J Biol Chem · December 25, 2009 Nitric oxide exerts a plethora of biological effects via protein S-nitrosylation, a redox-based reaction that converts a protein Cys thiol to a S-nitrosothiol. However, although the regulation of protein S-nitrosylation has been the subject of extensive st ... Full text Link to item Cite

Protection from lipopolysaccharide-induced lung injury by augmentation of airway S-nitrosothiols.

Journal Article Am J Respir Crit Care Med · July 1, 2009 RATIONALE: S-Nitrosothiols (SNO) inhibit immune activation of the respiratory epithelium and airway SNO levels are decreased in inflammatory lung disease. Ethyl nitrite (ENO) is a gas with chemical properties favoring SNO formation. Augmentation of airway ... Full text Link to item Cite

NOS2 regulation of NF-kappaB by S-nitrosylation of p65.

Journal Article J Biol Chem · October 19, 2007 Signal transduction in the NF-kappaB transcription factor pathway is inhibited by inducible nitric oxide synthase (NOS2) activity, although the molecular mechanism(s) are incompletely understood. We have previously shown that nitric oxide (NO), derived fro ... Full text Link to item Cite

Protein S-nitrosylation: purview and parameters.

Journal Article Nat Rev Mol Cell Biol · February 2005 S-nitrosylation, the covalent attachment of a nitrogen monoxide group to the thiol side chain of cysteine, has emerged as an important mechanism for dynamic, post-translational regulation of most or all main classes of protein. S-nitrosylation thereby conv ... Full text Link to item Cite

S-nitrosylation: physiological regulation of NF-kappaB.

Journal Article Proc Natl Acad Sci U S A · June 15, 2004 Full text Link to item Cite

Essential roles of S-nitrosothiols in vascular homeostasis and endotoxic shock.

Journal Article Cell · February 20, 2004 The current perspective of NO biology is formulated predominantly from studies of NO synthesis. The role of S-nitrosothiol (SNO) formation and turnover in governing NO-related bioactivity remains uncertain. We generated mice with a targeted gene deletion o ... Full text Link to item Cite

S-nitrosothiols in lung inflammation

Chapter · January 1, 2003 S-Nitrosothiols (SNOs) are complexes of nitric oxide (NO) bound to thiol groups, which are present in biological systems as proteins and low molecular weight peptides (1,2). SNOs have been regarded by some as markers of NO production [i.e., nitric oxide sy ... Cite

ERK-dependent proteasome degradation of Txnip regulates thioredoxin oxidoreductase activity.

Journal Article J Biol Chem · September 6, 2019 Dynamic control of thioredoxin (Trx) oxidoreductase activity is essential for balancing the need of cells to rapidly respond to oxidative/nitrosative stress and to temporally regulate thiol-based redox signaling. We have previously shown that cytokine stim ... Full text Link to item Cite

Thioredoxin-mediated denitrosylation regulates cytokine-induced nuclear factor κB (NF-κB) activation.

Journal Article J Biol Chem · January 31, 2014 S-nitrosylation of nuclear factor κB (NF-κB) on the p65 subunit of the p50/p65 heterodimer inhibits NF-κB DNA binding activity. We have recently shown that p65 is constitutively S-nitrosylated in the lung and that LPS-induced injury elicits a decrease in S ... Full text Link to item Cite

Proteomic analysis of the NOS2 interactome in human airway epithelial cells.

Journal Article Nitric Oxide · November 1, 2013 The cytokine-inducible isoform of nitric oxide synthase (NOS2) is constitutively expressed in human respiratory epithelia and is upregulated in inflammatory lung disease. Here, we sought to better define the protein interactions that may be important for N ... Full text Link to item Cite

Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.

Journal Article J Proteome Res · May 3, 2013 The analysis of airway fluid, as sampled by bronchoalveolar lavage (BAL), provides a minimally invasive route to interrogate lung biology in health and disease. Here, we used immunodepletion, coupled with gel- and label-free LC-MS/MS, for quantitation of t ... Full text Link to item Cite

Proteomic Analysis Of Human Bronchoalveolar Lavage Fluid After Subsegmental Challenge

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · 2013 Cite

S-nitrosylation of Ras in breast cancer.

Journal Article Breast Cancer Res · November 12, 2012 Elevated expression of nitric oxide synthase 2 has been recently shown to correlate with poor survival in estrogen receptor-negative breast cancer. In an article in Breast Cancer Research, Switzer and colleagues identify the transcription factor Ets-1 as a ... Full text Link to item Cite

S-nitrosylation in the regulation of gene transcription.

Journal Article Biochim Biophys Acta · June 2012 BACKGROUND: Post-translational modification of proteins by S-nitrosylation serves as a major mode of signaling in mammalian cells and a growing body of evidence has shown that transcription factors and their activating pathways are primary targets. S-nitro ... Full text Link to item Cite

The clinical and environmental determinants of airway transcriptional profiles in allergic asthma.

Journal Article Am J Respir Crit Care Med · March 15, 2012 RATIONALE: Gene expression profiling of airway epithelial and inflammatory cells can be used to identify genes involved in environmental asthma. METHODS: Airway epithelia and inflammatory cells were obtained via bronchial brush and bronchoalveolar lavage ( ... Full text Link to item Cite

NOS2 regulation of LPS-induced airway inflammation via S-nitrosylation of NF-{kappa}B p65.

Journal Article Am J Physiol Lung Cell Mol Physiol · September 2011 Inducible nitric oxide synthase (NOS2) expression is increased in the airway epithelium in acute inflammatory disorders although the physiological impact remains unclear. We have previously shown that NOS2 inhibits NF-κB (p50-p65) activation in respiratory ... Full text Link to item Cite

Quantitative Proteomics Of The Cytokine-Modulated Nos2 Interactome In Airway Epithelium

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2011 Link to item Cite

Thioredoxin-interacting protein (Txnip) is a feedback regulator of S-nitrosylation.

Journal Article J Biol Chem · December 25, 2009 Nitric oxide exerts a plethora of biological effects via protein S-nitrosylation, a redox-based reaction that converts a protein Cys thiol to a S-nitrosothiol. However, although the regulation of protein S-nitrosylation has been the subject of extensive st ... Full text Link to item Cite

Protection from lipopolysaccharide-induced lung injury by augmentation of airway S-nitrosothiols.

Journal Article Am J Respir Crit Care Med · July 1, 2009 RATIONALE: S-Nitrosothiols (SNO) inhibit immune activation of the respiratory epithelium and airway SNO levels are decreased in inflammatory lung disease. Ethyl nitrite (ENO) is a gas with chemical properties favoring SNO formation. Augmentation of airway ... Full text Link to item Cite

NOS2 regulation of NF-kappaB by S-nitrosylation of p65.

Journal Article J Biol Chem · October 19, 2007 Signal transduction in the NF-kappaB transcription factor pathway is inhibited by inducible nitric oxide synthase (NOS2) activity, although the molecular mechanism(s) are incompletely understood. We have previously shown that nitric oxide (NO), derived fro ... Full text Link to item Cite

Protein S-nitrosylation: purview and parameters.

Journal Article Nat Rev Mol Cell Biol · February 2005 S-nitrosylation, the covalent attachment of a nitrogen monoxide group to the thiol side chain of cysteine, has emerged as an important mechanism for dynamic, post-translational regulation of most or all main classes of protein. S-nitrosylation thereby conv ... Full text Link to item Cite

S-nitrosylation: physiological regulation of NF-kappaB.

Journal Article Proc Natl Acad Sci U S A · June 15, 2004 Full text Link to item Cite

Essential roles of S-nitrosothiols in vascular homeostasis and endotoxic shock.

Journal Article Cell · February 20, 2004 The current perspective of NO biology is formulated predominantly from studies of NO synthesis. The role of S-nitrosothiol (SNO) formation and turnover in governing NO-related bioactivity remains uncertain. We generated mice with a targeted gene deletion o ... Full text Link to item Cite

S-nitrosothiols in lung inflammation

Chapter · January 1, 2003 S-Nitrosothiols (SNOs) are complexes of nitric oxide (NO) bound to thiol groups, which are present in biological systems as proteins and low molecular weight peptides (1,2). SNOs have been regarded by some as markers of NO production [i.e., nitric oxide sy ... Cite

Nitrosative stress-induced apoptosis through inhibition of NF-kappa B.

Journal Article J Biol Chem · September 13, 2002 Nitrosative stress produced by cytokines predisposes to apoptotic cell death. However, the molecular mechanism by which this occurs is not well understood. We have shown previously that nitric oxide (NO) regulates the activity of the anti-apoptotic transcr ... Full text Link to item Cite

Inhibition of NF-kappa B by S-nitrosylation.

Journal Article Biochemistry · February 13, 2001 It is not clear if redox regulation of transcription is the consequence of direct redox-related modifications of transcription factors, or if it occurs at some other redox-sensitive step. One obstacle has been the inability to demonstrate redox-related mod ... Full text Link to item Cite

Nitrosation and oxidation in the regulation of gene expression.

Journal Article FASEB J · October 2000 A growing body of evidence suggests that the cellular response to oxidative and nitrosative stress is primarily regulated at the level of transcription. Posttranslational modification of transcription factors may provide a mechanism by which cells sense th ... Full text Link to item Cite

NO waiting to exhale in asthma.

Journal Article Am J Respir Crit Care Med · March 2000 Full text Link to item Cite

The stathmokinetic and morphological response of the hamster respiratory epithelium to intralaryngeal instillations of saline and ferric oxide in saline.

Journal Article Fundam Appl Toxicol · November 1987 Ferric oxide (Fe2O3) and saline, substances which enhance lung tumor development when intratracheally instilled with certain carcinogens, were studied for their effects on respiratory epithelial cell proliferation in Syrian golden hamsters. Groups of hamst ... Full text Link to item Cite

The stathmokinetic and morphological response of the hamster respiratory epithelium to intralaryngeal instillations of saline and ferric oxide in saline

Journal Article Fundamental and Applied Toxicology · 1987 Ferric oxide (Fe2O3) and saline, substances which enhance lung tumor development when intratracheally instilled with certain carcinogens, were studied for their effects on respiratory epithelial cell proliferation in Syrian golden hamsters. Groups of hamst ... Cite