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Matthew Wolf Foster

Associate Professor in Medicine
Medicine, Pulmonary, Allergy, and Critical Care Medicine
Duke Box 2613, Durham, NC 27710
Suite 320 Chesterfield Bldg, 701 W. Main St., Durham, NC 27701

Selected Publications


Nontargeted Plasma Proteomic Analysis of Renal Disease and Pulmonary Hypertension in Patients with Sickle Cell Disease.

Journal Article J Proteome Res · March 1, 2024 Sickle cell disease (SCD) is characterized by red blood cell sickling, vaso-occlusion, hemolytic anemia, damage to multiple organ systems, and, as a result, shortened life expectancy. Sickle cell disease nephropathy (SCDN) and pulmonary hypertension (pHTN) ... Full text Link to item Cite

Differences in the Circulating Proteome in Individuals with versus without Sickle Cell Trait.

Journal Article Clin J Am Soc Nephrol · November 1, 2023 BACKGROUND: Sickle cell trait affects approximately 8% of Black individuals in the United States, along with many other individuals with ancestry from malaria-endemic regions worldwide. While traditionally considered a benign condition, recent evidence sug ... Full text Link to item Cite

The urinary proteome infers dysregulation of mitochondrial, lysosomal, and protein reabsorption processes in chronic kidney disease of unknown etiology (CKDu).

Journal Article Am J Physiol Renal Physiol · April 1, 2023 Chronic kidney disease (CKD) of uncertain etiology (CKDu) is a global health concern affecting tropical farming communities. CKDu is not associated with typical risk factors (e.g., diabetes) and strongly correlates with environmental drivers. To gain poten ... Full text Link to item Cite

Octreotide may improve pharyngocutaneous fistula healing through downregulation of cystatins: A pilot study.

Journal Article Laryngoscope Investig Otolaryngol · February 2023 BACKGROUND: Pharyngocutaneous fistula (PCF) and salivary leaks are well known complications of head and neck surgery. The medical management of PCF has included the use of octreotide without a well-defined understanding of its therapeutic mechanism. We hyp ... Full text Link to item Cite

Multiomic, Mass Spectrometry-based Analysis of Dried Blood: Toward Deep Phenotyping of Sepsis

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · 2023 Cite

A Multidimensional Bioinformatic Platform for the Study of Human Response to Surgery.

Journal Article Ann Surg · June 1, 2022 OBJECTIVE: To design and establish a prospective biospecimen repository that integrates multi-omics assays with clinical data to study mechanisms of controlled injury and healing. BACKGROUND: Elective surgery is an opportunity to understand both the system ... Full text Link to item Cite

Branched-chain α-ketoacids are preferentially reaminated and activate protein synthesis in the heart.

Journal Article Nat Commun · March 15, 2021 Branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) are elevated in an array of cardiometabolic diseases. Here we demonstrate that the major metabolic fate of uniformly-13C-labeled α-ketoisovalerate ([U-13C]KIV) in the heart is reaminati ... Full text Link to item Cite

Characterization of Small-Molecule-Induced Changes in Parkinson's-Related Trafficking via the Nedd4 Ubiquitin Signaling Cascade.

Journal Article Cell Chem Biol · January 21, 2021 The benzdiimidazole NAB2 rescues α-synuclein-associated trafficking defects associated with early onset Parkinson's disease in a Nedd4-dependent manner. Despite identification of E3 ubiquitin ligase Nedd4 as a putative target of NAB2, its molecular mechani ... Full text Link to item Cite

The effect of pathogen inactivation on cryoprecipitate: a functional and quantitative evaluation.

Journal Article Blood Transfus · November 2020 BACKGROUND: As a pooled donor blood product, cryoprecipitate (cryo) carries risks of pathogen transmission. Pathogen inactivation (PI) improves the safety of cryoprecipitate, but its effects on haemostatic properties remain unclear. This study investigated ... Full text Link to item Cite

Interleukin-13 disrupts type 2 pneumocyte stem cell activity.

Journal Article JCI Insight · January 16, 2020 Featured Publication The T helper 2 (Th2) inflammatory cytokine interleukin-13 (IL-13) has been associated with both obstructive and fibrotic lung diseases; however, its specific effect on the epithelial stem cells in the gas exchange compartment of the lung (alveolar space) h ... Full text Link to item Cite

The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3.

Journal Article Cell Host Microbe · January 8, 2020 Bacteria masterfully co-opt and subvert host signal transduction. As a paradigmatic example, Salmonella uses two type-3 secretion systems to inject effector proteins that facilitate Salmonella entry, establishment of an intracellular niche, and modulation ... Full text Link to item Cite

ERK-dependent proteasome degradation of Txnip regulates thioredoxin oxidoreductase activity.

Journal Article J Biol Chem · September 6, 2019 Dynamic control of thioredoxin (Trx) oxidoreductase activity is essential for balancing the need of cells to rapidly respond to oxidative/nitrosative stress and to temporally regulate thiol-based redox signaling. We have previously shown that cytokine stim ... Full text Link to item Cite

Pharmacokinetic Analysis of a Novel Human EGFRvIII:CD3 Bispecific Antibody in Plasma and Whole Blood Using a High-Resolution Targeted Mass Spectrometry Approach.

Journal Article J Proteome Res · August 2, 2019 Bispecific single chain antibody fragments (bi-scFv) represent an emerging class of biotherapeutics. We recently developed a fully human bi-scFv (EGFRvIII:CD3 bi-scFv) with the goal of redirecting CD3-expressing T cells to recognize and destroy malignant, ... Full text Link to item Cite

A comparative analysis of egg provisioning using mass spectrometry during rapid life history evolution in sea urchins.

Journal Article Evol Dev · July 2019 A dramatic life history switch that has evolved numerous times in marine invertebrates is the transition from planktotrophic (feeding) to lecithotrophic (nonfeeding) larval development-an evolutionary tradeoff with many important developmental and ecologic ... Full text Open Access Link to item Cite

Identification of a Novel Inhibitor of Human Rhinovirus Replication and Inflammation in Airway Epithelial Cells.

Journal Article Am J Respir Cell Mol Biol · January 2019 Human rhinovirus (RV), the major cause of the common cold, triggers the majority of acute airway exacerbations in patients with asthma and chronic obstructive pulmonary disease. Nitric oxide, and the related metabolite S-nitrosoglutathione, are produced in ... Full text Link to item Cite

Macrophage cells secrete factors including LRP1 that orchestrate the rejuvenation of bone repair in mice.

Journal Article Nat Commun · December 5, 2018 The pace of repair declines with age and, while exposure to a young circulation can rejuvenate fracture repair, the cell types and factors responsible for rejuvenation are unknown. Here we report that young macrophage cells produce factors that promote ost ... Full text Open Access Link to item Cite

UBE2N Promotes Melanoma Growth via MEK/FRA1/SOX10 Signaling.

Journal Article Cancer Res · November 15, 2018 UBE2N is a K63-specific ubiquitin conjugase linked to various immune disorders and cancer. Here, we demonstrate that UBE2N and its partners UBE2V1 and UBE2V2 are highly expressed in malignant melanoma. Silencing of UBE2N and its partners significantly decr ... Full text Link to item Cite

Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae.

Journal Article Mol Biol Cell · November 1, 2018 In the budding yeast Saccharomyces cerevisiae, transcription factors (TFs) regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events. Experimental evidence coupled with ... Full text Link to item Cite

Nontargeted Plasma Proteomic Analysis of Renal Disease and Pulmonary Hypertension in Patients with Sickle Cell Disease.

Journal Article J Proteome Res · March 1, 2024 Sickle cell disease (SCD) is characterized by red blood cell sickling, vaso-occlusion, hemolytic anemia, damage to multiple organ systems, and, as a result, shortened life expectancy. Sickle cell disease nephropathy (SCDN) and pulmonary hypertension (pHTN) ... Full text Link to item Cite

Differences in the Circulating Proteome in Individuals with versus without Sickle Cell Trait.

Journal Article Clin J Am Soc Nephrol · November 1, 2023 BACKGROUND: Sickle cell trait affects approximately 8% of Black individuals in the United States, along with many other individuals with ancestry from malaria-endemic regions worldwide. While traditionally considered a benign condition, recent evidence sug ... Full text Link to item Cite

The urinary proteome infers dysregulation of mitochondrial, lysosomal, and protein reabsorption processes in chronic kidney disease of unknown etiology (CKDu).

Journal Article Am J Physiol Renal Physiol · April 1, 2023 Chronic kidney disease (CKD) of uncertain etiology (CKDu) is a global health concern affecting tropical farming communities. CKDu is not associated with typical risk factors (e.g., diabetes) and strongly correlates with environmental drivers. To gain poten ... Full text Link to item Cite

Octreotide may improve pharyngocutaneous fistula healing through downregulation of cystatins: A pilot study.

Journal Article Laryngoscope Investig Otolaryngol · February 2023 BACKGROUND: Pharyngocutaneous fistula (PCF) and salivary leaks are well known complications of head and neck surgery. The medical management of PCF has included the use of octreotide without a well-defined understanding of its therapeutic mechanism. We hyp ... Full text Link to item Cite

Multiomic, Mass Spectrometry-based Analysis of Dried Blood: Toward Deep Phenotyping of Sepsis

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · 2023 Cite

A Multidimensional Bioinformatic Platform for the Study of Human Response to Surgery.

Journal Article Ann Surg · June 1, 2022 OBJECTIVE: To design and establish a prospective biospecimen repository that integrates multi-omics assays with clinical data to study mechanisms of controlled injury and healing. BACKGROUND: Elective surgery is an opportunity to understand both the system ... Full text Link to item Cite

Branched-chain α-ketoacids are preferentially reaminated and activate protein synthesis in the heart.

Journal Article Nat Commun · March 15, 2021 Branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) are elevated in an array of cardiometabolic diseases. Here we demonstrate that the major metabolic fate of uniformly-13C-labeled α-ketoisovalerate ([U-13C]KIV) in the heart is reaminati ... Full text Link to item Cite

Characterization of Small-Molecule-Induced Changes in Parkinson's-Related Trafficking via the Nedd4 Ubiquitin Signaling Cascade.

Journal Article Cell Chem Biol · January 21, 2021 The benzdiimidazole NAB2 rescues α-synuclein-associated trafficking defects associated with early onset Parkinson's disease in a Nedd4-dependent manner. Despite identification of E3 ubiquitin ligase Nedd4 as a putative target of NAB2, its molecular mechani ... Full text Link to item Cite

The effect of pathogen inactivation on cryoprecipitate: a functional and quantitative evaluation.

Journal Article Blood Transfus · November 2020 BACKGROUND: As a pooled donor blood product, cryoprecipitate (cryo) carries risks of pathogen transmission. Pathogen inactivation (PI) improves the safety of cryoprecipitate, but its effects on haemostatic properties remain unclear. This study investigated ... Full text Link to item Cite

Interleukin-13 disrupts type 2 pneumocyte stem cell activity.

Journal Article JCI Insight · January 16, 2020 Featured Publication The T helper 2 (Th2) inflammatory cytokine interleukin-13 (IL-13) has been associated with both obstructive and fibrotic lung diseases; however, its specific effect on the epithelial stem cells in the gas exchange compartment of the lung (alveolar space) h ... Full text Link to item Cite

The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3.

Journal Article Cell Host Microbe · January 8, 2020 Bacteria masterfully co-opt and subvert host signal transduction. As a paradigmatic example, Salmonella uses two type-3 secretion systems to inject effector proteins that facilitate Salmonella entry, establishment of an intracellular niche, and modulation ... Full text Link to item Cite

ERK-dependent proteasome degradation of Txnip regulates thioredoxin oxidoreductase activity.

Journal Article J Biol Chem · September 6, 2019 Dynamic control of thioredoxin (Trx) oxidoreductase activity is essential for balancing the need of cells to rapidly respond to oxidative/nitrosative stress and to temporally regulate thiol-based redox signaling. We have previously shown that cytokine stim ... Full text Link to item Cite

Pharmacokinetic Analysis of a Novel Human EGFRvIII:CD3 Bispecific Antibody in Plasma and Whole Blood Using a High-Resolution Targeted Mass Spectrometry Approach.

Journal Article J Proteome Res · August 2, 2019 Bispecific single chain antibody fragments (bi-scFv) represent an emerging class of biotherapeutics. We recently developed a fully human bi-scFv (EGFRvIII:CD3 bi-scFv) with the goal of redirecting CD3-expressing T cells to recognize and destroy malignant, ... Full text Link to item Cite

A comparative analysis of egg provisioning using mass spectrometry during rapid life history evolution in sea urchins.

Journal Article Evol Dev · July 2019 A dramatic life history switch that has evolved numerous times in marine invertebrates is the transition from planktotrophic (feeding) to lecithotrophic (nonfeeding) larval development-an evolutionary tradeoff with many important developmental and ecologic ... Full text Open Access Link to item Cite

Identification of a Novel Inhibitor of Human Rhinovirus Replication and Inflammation in Airway Epithelial Cells.

Journal Article Am J Respir Cell Mol Biol · January 2019 Human rhinovirus (RV), the major cause of the common cold, triggers the majority of acute airway exacerbations in patients with asthma and chronic obstructive pulmonary disease. Nitric oxide, and the related metabolite S-nitrosoglutathione, are produced in ... Full text Link to item Cite

Macrophage cells secrete factors including LRP1 that orchestrate the rejuvenation of bone repair in mice.

Journal Article Nat Commun · December 5, 2018 The pace of repair declines with age and, while exposure to a young circulation can rejuvenate fracture repair, the cell types and factors responsible for rejuvenation are unknown. Here we report that young macrophage cells produce factors that promote ost ... Full text Open Access Link to item Cite

UBE2N Promotes Melanoma Growth via MEK/FRA1/SOX10 Signaling.

Journal Article Cancer Res · November 15, 2018 UBE2N is a K63-specific ubiquitin conjugase linked to various immune disorders and cancer. Here, we demonstrate that UBE2N and its partners UBE2V1 and UBE2V2 are highly expressed in malignant melanoma. Silencing of UBE2N and its partners significantly decr ... Full text Link to item Cite

Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae.

Journal Article Mol Biol Cell · November 1, 2018 In the budding yeast Saccharomyces cerevisiae, transcription factors (TFs) regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events. Experimental evidence coupled with ... Full text Link to item Cite

Bronchoalveolar Lavage Fluid Proteome in Chronic Lung Allograft Dysfunction

Conference The Journal of Heart and Lung Transplantation · April 2018 Full text Cite

S-Nitrosoglutathione Reductase Inhibitor Blockade of Rhinovirus A16 Replication by Targeting ICAM-1

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2018 Link to item Cite

New Strategies and Challenges in Lung Proteomics and Metabolomics. An Official American Thoracic Society Workshop Report.

Conference Ann Am Thorac Soc · December 2017 This document presents the proceedings from the workshop entitled, "New Strategies and Challenges in Lung Proteomics and Metabolomics" held February 4th-5th, 2016, in Denver, Colorado. It was sponsored by the National Heart Lung Blood Institute, the Americ ... Full text Link to item Cite

The Diacetyl-Exposed Human Airway Epithelial Secretome: New Insights into Flavoring-Induced Airways Disease.

Journal Article Am J Respir Cell Mol Biol · June 2017 Bronchiolitis obliterans (BO) is an increasingly important lung disease characterized by fibroproliferative airway lesions and decrements in lung function. Occupational exposure to the artificial food flavoring ingredient diacetyl, commonly used to impart ... Full text Link to item Cite

Proteomic Analysis of Primary Human Airway Epithelial Cells Exposed to the Respiratory Toxicant Diacetyl.

Journal Article J Proteome Res · February 3, 2017 Occupational exposures to the diketone flavoring agent, diacetyl, have been associated with bronchiolitis obliterans, a rare condition of airway fibrosis. Model studies in rodents have suggested that the airway epithelium is a major site of diacetyl toxici ... Full text Link to item Cite

Proteomics Of Bronchoalveolar Lavage (bal) Fluid In Bronchiolitis Obliterans Syndrome (bos)

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2017 Link to item Cite

Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.

Journal Article Sci Rep · September 27, 2016 On Earth, biological systems have evolved in response to environmental stressors, interactions dictated by physical forces that include gravity. The absence of gravity is an extreme stressor and the impact of its absence on biological systems is ill-define ... Full text Open Access Link to item Cite

Synthesis Of A Cross-Linked Hyaluronan Matrix By The Airway Epithelium In Experimental Bronchiolitis Obliterans

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2016 Link to item Cite

Proteomic Analysis Reveals Significant Epithelial Remodeling After In Vitro Exposure To Diacetyl Vapor

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2016 Link to item Cite

Plasma Metabolomics Analysis Identifies Candidate Biomarkers Of Flavoring-Induced Bronchiolitis Obliterans

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2016 Link to item Cite

A Gsnor Inhibitor Blocks Hrv Infection Of Primary Human Airway Epithelial Cells

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2016 Link to item Cite

S-Nitrosylation of Sarcomeric Proteins Depresses Myofilament Ca2+)Sensitivity in Intact Cardiomyocytes.

Journal Article Antioxid Redox Signal · November 1, 2015 AIMS: The heart responds to physiological and pathophysiological stress factors by increasing its production of nitric oxide (NO), which reacts with intracellular glutathione to form S-nitrosoglutathione (GSNO), a protein S-nitrosylating agent. Although S- ... Full text Link to item Cite

Targeted Proteomics of Human Metapneumovirus in Clinical Samples and Viral Cultures.

Journal Article Anal Chem · October 20, 2015 Featured Publication The rapid, sensitive, and specific identification of infectious pathogens from clinical isolates is a critical need in the hospital setting. Mass spectrometry (MS) has been widely adopted for identification of bacterial pathogens, although polymerase chain ... Full text Link to item Cite

Cerebrospinal fluid proteomics in children during induction for acute lymphoblastic leukemia: A pilot study.

Journal Article Pediatr Blood Cancer · July 2015 BACKGROUND: Thrombosis in patients with acute lymphocytic leukemia (ALL) can develop after treatment with L-asparaginase (asp) and is often localized to the central nervous system (CNS). We hypothesize that changes in the cerebrospinal fluid (CSF) proteome ... Full text Link to item Cite

Quantitative proteomics of bronchoalveolar lavage fluid in idiopathic pulmonary fibrosis.

Journal Article J Proteome Res · February 6, 2015 Featured Publication The proteomic analysis of bronchoalveolar lavage fluid (BALF) can give insight into pulmonary disease pathology and response to therapy. Here, we describe the first gel-free quantitative analysis of BALF in idiopathic pulmonary fibrosis (IPF), a chronic an ... Full text Link to item Cite

Eosinophil-associated lung diseases. A cry for surfactant proteins A and D help?

Journal Article Am J Respir Cell Mol Biol · November 2014 Surfactant proteins (SP)-A and SP-D (SP-A/-D) play important roles in numerous eosinophil-dominated diseases, including asthma, allergic bronchopulmonary aspergillosis, and allergic rhinitis. In these settings, SP-A/-D have been shown to modulate eosinophi ... Full text Link to item Cite

Identification and Quantitation of Coding Variants and Isoforms of Pulmonary Surfactant Protein A.

Journal Article J Proteome Res · August 1, 2014 Featured Publication Pulmonary surfactant protein A (SP-A), a heterooligomer of SP-A1 and SP-A2, is an important regulator of innate immunity of the lung. Nonsynonymous single nucleotide variants of SP-A have been linked to respiratory diseases, but the expressed repertoire of ... Full text Link to item Cite

Thioredoxin-mediated denitrosylation regulates cytokine-induced nuclear factor κB (NF-κB) activation.

Journal Article J Biol Chem · January 31, 2014 S-nitrosylation of nuclear factor κB (NF-κB) on the p65 subunit of the p50/p65 heterodimer inhibits NF-κB DNA binding activity. We have recently shown that p65 is constitutively S-nitrosylated in the lung and that LPS-induced injury elicits a decrease in S ... Full text Link to item Cite

Phosphoproteomic profiling of human myocardial tissues distinguishes ischemic from non-ischemic end stage heart failure.

Journal Article PLoS One · 2014 The molecular differences between ischemic (IF) and non-ischemic (NIF) heart failure are poorly defined. A better understanding of the molecular differences between these two heart failure etiologies may lead to the development of more effective heart fail ... Full text Open Access Link to item Cite

Longitudinal study of differential protein expression in an Alzheimer's mouse model lacking inducible nitric oxide synthase.

Journal Article J Proteome Res · October 4, 2013 Alzheimer's disease (AD) is a complex neurodegenerative process that involves altered brain immune, neuronal and metabolic functions. Understanding the underlying mechanisms has relied on mouse models that mimic components of AD pathology. We used gel-free ... Full text Link to item Cite

Solid-phase capture for the detection and relative quantification of S-nitrosoproteins by mass spectrometry.

Journal Article Methods · August 1, 2013 The proteomic analysis of S-nitrosylated protein (SNO-proteins) has long depended on the biotin switch technique (BST), which requires blocking of free thiols, ascorbate-based denitrosylation of SNO-Cys, biotinylation of nascent thiol and avidin-based affi ... Full text Link to item Cite

Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.

Journal Article J Proteome Res · May 3, 2013 Featured Publication The analysis of airway fluid, as sampled by bronchoalveolar lavage (BAL), provides a minimally invasive route to interrogate lung biology in health and disease. Here, we used immunodepletion, coupled with gel- and label-free LC-MS/MS, for quantitation of t ... Full text Link to item Cite

Proteomic Analysis Of Human Bronchoalveolar Lavage Fluid After Subsegmental Challenge

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · 2013 Cite

S-nitrosylation of Ras in breast cancer.

Journal Article Breast Cancer Res · November 12, 2012 Elevated expression of nitric oxide synthase 2 has been recently shown to correlate with poor survival in estrogen receptor-negative breast cancer. In an article in Breast Cancer Research, Switzer and colleagues identify the transcription factor Ets-1 as a ... Full text Link to item Cite

Response to "is flavohemoglobin a nitric oxide dioxygenase?"

Journal Article Free Radical Biology and Medicine · September 1, 2012 Full text Cite

Methodologies for the characterization, identification and quantification of S-nitrosylated proteins.

Journal Article Biochim Biophys Acta · June 2012 Featured Publication BACKGROUND: Protein S-nitrosylation plays a central role in signal transduction by nitric oxide (NO), and aberrant S-nitrosylation of specific proteins is increasingly implicated in disease. SCOPE OF REVIEW: Here, methodologies for the characterization, id ... Full text Link to item Cite

Nitric oxide reduces NADPH oxidase 5 (Nox5) activity by reversible S-nitrosylation.

Journal Article Free Radic Biol Med · May 1, 2012 The NADPH oxidases (Noxs) are a family of transmembrane oxidoreductases that produce superoxide and other reactive oxygen species (ROS). Nox5 was the last of the conventional Nox isoforms to be identified and is a calcium-dependent enzyme that does not dep ... Full text Link to item Cite

Protection from nitrosative stress: a central role for microbial flavohemoglobin.

Journal Article Free Radic Biol Med · May 1, 2012 Nitric oxide (NO) is an inevitable product of life in an oxygen- and nitrogen-rich environment. This reactive diatomic molecule exhibits microbial cytotoxicity, in large part by facilitating nitrosative stress and inhibiting heme-containing proteins within ... Full text Link to item Cite

Proteomic characterization of the cellular response to nitrosative stress mediated by s-nitrosoglutathione reductase inhibition.

Journal Article J Proteome Res · April 6, 2012 The S-nitrosoglutathione-metabolizing enzyme, GSNO reductase (GSNOR), has emerged as an important regulator of protein S-nitrosylation. GSNOR ablation is protective in models of asthma and heart failure, raising the idea that GSNOR inhibitors might hold th ... Full text Link to item Cite

Dynamic denitrosylation via S-nitrosoglutathione reductase regulates cardiovascular function.

Journal Article Proc Natl Acad Sci U S A · March 13, 2012 Although protein S-nitrosylation is increasingly recognized as mediating nitric oxide (NO) signaling, roles for protein denitrosylation in physiology remain unknown. Here, we show that S-nitrosoglutathione reductase (GSNOR), an enzyme that governs levels o ... Full text Link to item Cite

Analysis of oxygen/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics.

Journal Article J Proteome Res · February 3, 2012 Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min follow ... Full text Open Access Link to item Cite

S-nitrosoglutathione supplementation to ovalbumin-sensitized and -challenged mice ameliorates methacholine-induced bronchoconstriction.

Journal Article Am J Physiol Lung Cell Mol Physiol · November 2011 Featured Publication S-nitrosoglutathione (GSNO) is an endogenous bronchodilator present in micromolar concentrations in airway lining fluid. Airway GSNO levels decrease in severe respiratory failure and asthma, which is attributable to increased metabolism by GSNO reductase ( ... Full text Link to item Cite

NOS2 regulation of LPS-induced airway inflammation via S-nitrosylation of NF-{kappa}B p65.

Journal Article Am J Physiol Lung Cell Mol Physiol · September 2011 Inducible nitric oxide synthase (NOS2) expression is increased in the airway epithelium in acute inflammatory disorders although the physiological impact remains unclear. We have previously shown that NOS2 inhibits NF-κB (p50-p65) activation in respiratory ... Full text Link to item Cite

Quantitative Proteomics Of The Cytokine-Modulated Nos2 Interactome In Airway Epithelium

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2011 Link to item Cite

A Proteomic Investigation Of GSNOR-Regulated Pathways In Cytokine-Stimulated Mouse Alveolar Macrophages

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2011 Link to item Cite

Thioredoxin-interacting protein (Txnip) is a feedback regulator of S-nitrosylation.

Journal Article J Biol Chem · December 25, 2009 Featured Publication Nitric oxide exerts a plethora of biological effects via protein S-nitrosylation, a redox-based reaction that converts a protein Cys thiol to a S-nitrosothiol. However, although the regulation of protein S-nitrosylation has been the subject of extensive st ... Full text Link to item Cite

A protein microarray-based analysis of S-nitrosylation.

Journal Article Proc Natl Acad Sci U S A · November 10, 2009 Featured Publication The ubiquitous cellular influence of nitric oxide (NO) is exerted substantially through protein S-nitrosylation. Whereas NO is highly promiscuous, physiological S-nitrosylation is typically restricted to one or very few Cys residue(s) in target proteins. T ... Full text Link to item Cite

Protein S-nitrosylation in health and disease: a current perspective.

Journal Article Trends Mol Med · September 2009 Featured Publication Protein S-nitrosylation constitutes a large part of the ubiquitous influence of nitric oxide on cellular signal transduction and accumulating evidence indicates important roles for S-nitrosylation both in normal physiology and in a broad spectrum of human ... Full text Link to item Cite

Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.

Journal Article Nat Biotechnol · June 2009 We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilit ... Full text Link to item Cite

A genetic analysis of nitrosative stress.

Journal Article Biochemistry · February 3, 2009 Featured Publication Nitrosative stress is induced by pathophysiological levels of nitric oxide (NO) and S-nitrosothiols (e.g., S-nitrosoglutathione, GSNO) and arises, at least in significant part, from the nitrosylation of critical protein Cys thiols (S-nitrosylation) and met ... Full text Link to item Cite

Detection of protein S-nitrosylation with the biotin-switch technique.

Journal Article Free Radic Biol Med · January 15, 2009 Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in organisms from bacteria to human ... Full text Link to item Cite

Assessment and application of the biotin switch technique for examining protein S-nitrosylation under conditions of pharmacologically induced oxidative stress.

Journal Article J Biol Chem · May 11, 2007 Featured Publication Protein S-nitrosylation has emerged as a principal mechanism by which nitric oxide exerts biological effects. Among methods for studying protein S-nitrosylation, the biotin switch technique (BST) has rapidly gained popularity because of the ease with which ... Full text Link to item Cite

Regulation of beta-adrenergic receptor signaling by S-nitrosylation of G-protein-coupled receptor kinase 2.

Journal Article Cell · May 4, 2007 Featured Publication beta-adrenergic receptors (beta-ARs), prototypic G-protein-coupled receptors (GPCRs), play a critical role in regulating numerous physiological processes. The GPCR kinases (GRKs) curtail G-protein signaling and target receptors for internalization. Nitric ... Full text Link to item Cite

S-nitrosylation TRiPs a calcium switch.

Journal Article Nat Chem Biol · November 2006 Featured Publication Full text Link to item Cite

An essential role for mitochondrial aldehyde dehydrogenase in nitroglycerin bioactivation.

Journal Article Proc Natl Acad Sci U S A · August 23, 2005 Featured Publication The identity of the cellular mechanisms through which nitroglycerin (glyceryl trinitrate, GTN) elicits nitric oxide (NO)-based signaling to dilate blood vessels remains one of the longest standing foci of investigation and sources of controversy in cardiov ... Full text Link to item Cite

Role of circulating S-nitrosothiols in control of blood pressure.

Journal Article Hypertension · January 2005 Featured Publication Full text Link to item Cite

New insights into protein S-nitrosylation. Mitochondria as a model system.

Journal Article J Biol Chem · June 11, 2004 Featured Publication The biological effects of nitric oxide (NO) are in significant part mediated through S-nitrosylation of cysteine thiol. Work on model thiol substrates has raised the idea that molecular oxygen (O(2)) is required for S-nitrosylation by NO; however, the rele ... Full text Link to item Cite

S-nitrosylation in health and disease.

Journal Article Trends Mol Med · April 2003 Featured Publication S-nitrosylation is a ubiquitous redox-related modification of cysteine thiol by nitric oxide (NO), which transduces NO bioactivity. Accumulating evidence suggests that the products of S-nitrosylation, S-nitrosothiols (SNOs), play key roles in human health ... Full text Link to item Cite

Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe.

Journal Article Biochemistry · April 16, 2002 Genetic studies of bacteria and eukaryotes have led to identification of several gene products that are involved in the biosynthesis of protein-bound iron-sulfur clusters. One of these proteins, ISU, is homologous to the N-terminus of bacterial NifU. The m ... Full text Link to item Cite

Elucidation of a [4Fe-4S] cluster degradation pathway: rapid kinetic studies of the degradation of Chromatium vinosum HiPIP.

Journal Article J Biol Inorg Chem · March 2001 Featured Publication Irreversible disassembly of the 4Fe-4S cluster in Chromatium vinosum high-potential iron protein (HiPIP) has been investigated in the presence of a low concentration of guanidinium hydrochloride. From the dependence of degradation rate on [H+], it is deduc ... Full text Link to item Cite

Chemistry of nitric oxide with protein-bound iron sulfur centers. Insights on physiological reactivity

Journal Article Journal of the American Chemical Society · May 5, 1999 The anaerobic reaction of C. vinosum high-potential iron protein (HiPIP) with nitric oxide has been studied in order to understand the chemical reactivity of NO with protein-bound iron-sulfur clusters. Despite having a solvent inaccessible 4Fe-4S center, n ... Cite

Chemistry of nitric oxide with protein-bound iron sulfur centers. Insights on physiological reactivity

Journal Article Journal of the American Chemical Society · May 5, 1999 The anaerobic reaction of C. vinosum high-potential iron protein (HiPIP) with nitric oxide has been studied in order to understand the chemical reactivity of NO with protein-bound iron-sulfur clusters. Despite having a solvent inaccessible 4Fe-4S center, n ... Full text Cite

Mechanism of metal-promoted catalysis of nucleic acid hydrolysis by Escherichia coli ribonuclease H: Use of inert chromium complexes to evaluate hydrogen bonding and electrostatic stabilization of the transition state

Journal Article Journal of Biological Inorganic Chemistry · January 1, 1996 Catalytic activation of Escherichia coli ribonuclease H by a series of inert chromium complexes [Cr(NH3)6-x(H2O)x]3+ (x = 0-6) that bear water and ammine ligands in well-defined geometries in the inner coordination shell has been examined. Such complexes a ... Full text Cite