Scholars@Duke
Purushothama Rao Tata

Purushothama Rao Tata

Associate Professor of Cell Biology
Cell Biology

Selected Publications

Polybacterial Intracellular Macromolecules Shape Single-Cell Epikine Profiles in Upper Airway Mucosa.

Journal Article bioRxiv · October 9, 2024 The upper airway, particularly the nasal and oral mucosal epithelium, serves as a primary barrier for microbial interactions throughout life. Specialized niches like the anterior nares and the tooth are especially susceptible to dysbiosis and chronic infla ... Full text Link to item Cite

Topology-Driven Discovery of Transmembrane Protein S-Palmitoylation.

Journal Article bioRxiv · September 8, 2024 Protein S-palmitoylation is a reversible lipophilic posttranslational modification regulating a diverse number of signaling pathways. Within transmembrane proteins (TMPs), S-palmitoylation is implicated in conditions from inflammatory disorders to respirat ... Full text Link to item Cite

Analysis of Protein Cysteine Acylation Using a Modified Suspension Trap (Acyl-Trap).

Journal Article J Proteome Res · August 2, 2024 Proteins undergo reversible S-acylation via a thioester linkage in vivo. S-palmitoylation, modification by C16:0 fatty acid, is a common S-acylation that mediates critical protein-membrane and protein-protein interactions. The most widely used S-acylation ... Full text Link to item Cite

Airway basal stem cells are necessary for the maintenance of functional intraepithelial airway macrophages.

Journal Article bioRxiv · June 26, 2024 Adult stem cells play a crucial role in tissue homeostasis and repair through multiple mechanisms. In addition to being able to replace aged or damaged cells, stem cells provide signals that contribute to the maintenance and function of neighboring cells. ... Full text Link to item Cite

SARS-CoV-2 variant of concern fitness and adaptation in primary human airway epithelia.

Journal Article Cell Rep · April 23, 2024 The severe acute respiratory syndrome coronavirus 2 pandemic is characterized by the emergence of novel variants of concern (VOCs) that replace ancestral strains. Here, we dissect the complex selective pressures by evaluating variant fitness and adaptation ... Full text Link to item Cite

Stem cell migration drives lung repair in living mice.

Journal Article Dev Cell · April 8, 2024 Tissue repair requires a highly coordinated cellular response to injury. In the lung, alveolar type 2 cells (AT2s) act as stem cells to replenish both themselves and alveolar type 1 cells (AT1s); however, the complex orchestration of stem cell activity aft ... Full text Link to item Cite

Altered ontogeny and transcriptomic signatures of tissue-resident pulmonary interstitial macrophages ameliorate allergic airway hyperresponsiveness.

Journal Article Front Immunol · 2024 INTRODUCTION: Environmental exposures and experimental manipulations can alter the ontogenetic composition of tissue-resident macrophages. However, the impact of these alterations on subsequent immune responses, particularly in allergic airway diseases, re ... Full text Link to item Cite

Meta-Analysis of COVID-19 BAL Single-Cell RNA Sequencing Reveals Alveolar Epithelial Transitions and Unique Alveolar Epithelial Cell Fates.

Journal Article Am J Respir Cell Mol Biol · December 2023 Single-cell RNA sequencing (scRNA-seq) of BAL cells has provided insights into coronavirus disease (COVID-19). However, reports have been limited by small patient cohorts. We performed a meta-analysis of BAL scRNA-seq data from healthy control subjects (n  ... Full text Open Access Link to item Cite

Recent advances in lung organoid development and applications in disease modeling.

Journal Article J Clin Invest · November 15, 2023 Over the last decade, several organoid models have evolved to acquire increasing cellular, structural, and functional complexity. Advanced lung organoid platforms derived from various sources, including adult, fetal, and induced pluripotent stem cells, hav ... Full text Open Access Link to item Cite

Transitional cell states sculpt tissue topology during lung regeneration.

Journal Article Cell Stem Cell · November 2, 2023 Organ regeneration requires dynamic cell interactions to reestablish cell numbers and tissue architecture. While we know the identity of progenitor cells that replace lost tissue, the transient states they give rise to and their role in repair remain elusi ... Full text Link to item Cite

Host range, transmissibility and antigenicity of a pangolin coronavirus.

Journal Article Nat Microbiol · October 2023 The pathogenic and cross-species transmission potential of SARS-CoV-2-related coronaviruses (CoVs) remain poorly characterized. Here we recovered a wild-type pangolin (Pg) CoV GD strain including derivatives encoding reporter genes using reverse genetics. ... Full text Open Access Link to item Cite

A spatially resolved extracellular matrix proteome atlas of the distal human lung

Conference Mechanisms of lung injury and repair · September 9, 2023 Full text Cite

Efficient Adeno-associated Virus-mediated Transgenesis in Alveolar Stem Cells and Associated Niches.

Journal Article Am J Respir Cell Mol Biol · September 2023 Targeted delivery of transgenes to tissue-resident stem cells and related niches offers avenues for interrogating pathways and editing endogenous alleles for therapeutic interventions. Here, we survey multiple adeno-associated virus (AAV) serotypes, admini ... Full text Link to item Cite

GAA deficiency disrupts distal airway cells in Pompe disease.

Journal Article Am J Physiol Lung Cell Mol Physiol · September 1, 2023 Pompe disease is an autosomal recessive glycogen storage disease caused by mutations in the gene that encodes acid alpha-glucosidase (GAA)-an enzyme responsible for hydrolyzing lysosomal glycogen. GAA deficiency results in systemic lysosomal glycogen accum ... Full text Link to item Cite

An interview with Purushothama Rao Tata.

Journal Article Development · August 1, 2023 Purushothama Rao Tata is an Assistant Professor in the Department of Cell Biology at Duke University Medical Center. His lab is focussed on understanding the cellular basis of cell plasticity and self-organization in regeneration and tumorigenesis, using t ... Full text Link to item Cite

Regionally distinct progenitor cells in the lower airway give rise to neuroendocrine and multiciliated cells in the developing human lung.

Journal Article Proc Natl Acad Sci U S A · June 13, 2023 Using scRNA-seq and microscopy, we describe a cell that is enriched in the lower airways of the developing human lung and identified by the unique coexpression of SCGB3A2/SFTPB/CFTR. To functionally interrogate these cells, we apply a single-cell barcode-b ... Full text Open Access Link to item Cite

An integrated cell atlas of the lung in health and disease.

Journal Article Nat Med · June 2023 Single-cell technologies have transformed our understanding of human tissues. Yet, studies typically capture only a limited number of donors and disagree on cell type definitions. Integrating many single-cell datasets can address these limitations of indiv ... Full text Open Access Link to item Cite

Alveolar Cell Pathology in Pompe Disease

Conference B54. EARLY ORIGINS OF PEDIATRIC LUNG DISEASE · May 2023 Full text Cite

Progress and challenges in stem cell biology.

Journal Article Nat Cell Biol · February 2023 Full text Link to item Cite

Sex differences in resilience to ferroptosis underlie sexual dimorphism in kidney injury and repair.

Journal Article Cell Rep · November 8, 2022 In both humans and mice, repair of acute kidney injury is worse in males than in females. Here, we provide evidence that this sexual dimorphism results from sex differences in ferroptosis, an iron-dependent, lipid-peroxidation-driven regulated cell death. ... Full text Open Access Link to item Cite

Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

Journal Article Sci Signal · October 25, 2022 Multiple coronaviruses have emerged independently in the past 20 years that cause lethal human diseases. Although vaccine development targeting these viruses has been accelerated substantially, there remain patients requiring treatment who cannot be vaccin ... Full text Open Access Link to item Cite

Multi-apical polarity of alveolar stem cells and their dynamics during lung development and regeneration.

Journal Article iScience · October 21, 2022 Epithelial cells of diverse tissues are characterized by the presence of a single apical domain. In the lung, electron microscopy studies have suggested that alveolar type-2 epithelial cells (AT2s) en face multiple alveolar sacs. However, apical and basola ... Full text Open Access Link to item Cite

Lung Regeneration: Cells, Models, and Mechanisms.

Journal Article Cold Spring Harb Perspect Biol · October 3, 2022 Lung epithelium, the lining that covers the inner surface of the respiratory tract, is directly exposed to the environment and thus susceptible to airborne toxins, irritants, and pathogen-induced damages. In adult mammalian lungs, epithelial cells are gene ... Full text Open Access Link to item Cite

A Roadmap for the Human Oral and Craniofacial Cell Atlas.

Journal Article J Dent Res · October 2022 Oral and craniofacial tissues are uniquely adapted for continuous and intricate functioning, including breathing, feeding, and communication. To achieve these vital processes, this complex is supported by incredible tissue diversity, variously composed of ... Full text Open Access Link to item Cite

Coupled myovascular expansion directs cardiac growth and regeneration.

Journal Article Development · September 15, 2022 Heart regeneration requires multiple cell types to enable cardiomyocyte (CM) proliferation. How these cells interact to create growth niches is unclear. Here, we profile proliferation kinetics of cardiac endothelial cells (CECs) and CMs in the neonatal mou ... Full text Link to item Cite

Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.

Journal Article STAR Protoc · June 17, 2022 Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and str ... Full text Open Access Link to item Cite

Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations.

Journal Article Cell Rep · May 10, 2022 Mesenchymal progenitors of the lateral plate mesoderm give rise to various cell fates within limbs, including a heterogeneous group of muscle-resident mesenchymal cells. Often described as fibro-adipogenic progenitors, these cells are key players in muscle ... Full text Open Access Link to item Cite

GAA Deficiency Disrupts Distal Airway Cells in Pompe Disease

Journal Article FASEB journal : official publication of the Federation of American Societies for Experimental Biology · May 1, 2022 Pompe disease is an autosomal recessive glycogen storage disease caused by mutations in alpha-glucosidase (GAA) - an enzyme responsible of hydrolyzing lysosomal glycogen. GAA deficiency results in systemic lysosomal glycogen accumulation and cellular disru ... Full text Cite

Human distal lung maps and lineage hierarchies reveal a bipotent progenitor.

Journal Article Nature · April 2022 Mapping the spatial distribution and molecular identity of constituent cells is essential for understanding tissue dynamics in health and disease. We lack a comprehensive map of human distal airways, including the terminal and respiratory bronchioles (TRBs ... Full text Open Access Link to item Cite

Epigenetic basis of oncogenic-Kras-mediated epithelial-cellular proliferation and plasticity.

Journal Article Dev Cell · February 7, 2022 Oncogenic Kras induces a hyper-proliferative state that permits cells to progress to neoplasms in diverse epithelial tissues. Depending on the cell of origin, this also involves lineage transformation. Although a multitude of downstream factors have been i ... Full text Open Access Link to item Cite

An In Vitro Microfluidic Alveolus Model to Study Lung Biomechanics.

Journal Article Front Bioeng Biotechnol · 2022 The gas exchange units of the lung, the alveoli, are mechanically active and undergo cyclic deformation during breathing. The epithelial cells that line the alveoli contribute to lung function by reducing surface tension via surfactant secretion, which is ... Full text Open Access Link to item Cite

Chromatin Remodeling of Colorectal Cancer Liver Metastasis is Mediated by an HGF-PU.1-DPP4 Axis.

Journal Article Adv Sci (Weinh) · October 2021 Colorectal cancer (CRC) metastasizes mainly to the liver, which accounts for the majority of CRC-related deaths. Here it is shown that metastatic cells undergo specific chromatin remodeling in the liver. Hepatic growth factor (HGF) induces phosphorylation ... Full text Open Access Link to item Cite

Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair.

Journal Article Elife · July 19, 2021 Overwhelming lipid peroxidation induces ferroptotic stress and ferroptosis, a non-apoptotic form of regulated cell death that has been implicated in maladaptive renal repair in mice and humans. Using single-cell transcriptomic and mouse genetic approaches, ... Full text Open Access Link to item Cite

Chronic lung diseases are associated with gene expression programs favoring SARS-CoV-2 entry and severity.

Journal Article Nat Commun · July 14, 2021 Patients with chronic lung disease (CLD) have an increased risk for severe coronavirus disease-19 (COVID-19) and poor outcomes. Here, we analyze the transcriptomes of 611,398 single cells isolated from healthy and CLD lungs to identify molecular characteri ... Full text Open Access Link to item Cite

Epithelial cell plasticity: breaking boundaries and changing landscapes.

Journal Article EMBO Rep · July 5, 2021 Epithelial tissues respond to a wide variety of environmental and genotoxic stresses. As an adaptive mechanism, cells can deviate from their natural paths to acquire new identities, both within and across lineages. Under extreme conditions, epithelial tiss ... Full text Open Access Link to item Cite

COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets.

Journal Article Nature · July 2021 COVID-19, which is caused by SARS-CoV-2, can result in acute respiratory distress syndrome and multiple organ failure1-4, but little is known about its pathophysiology. Here we generated single-cell atlases of 24 lung, 16 kidney, 16 liver and 19 heart auto ... Full text Open Access Link to item Cite

Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia.

Journal Article Am J Respir Crit Care Med · May 15, 2021 Rationale: Identification of the specific cell types expressing CFTR (cystic fibrosis [CF] transmembrane conductance regulator) is required for precision medicine therapies for CF. However, a full characterization of CFTR expression in normal human airway ... Full text Link to item Cite

Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics.

Journal Article Nat Med · March 2021 Angiotensin-converting enzyme 2 (ACE2) and accessory proteases (TMPRSS2 and CTSL) are needed for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cellular entry, and their expression may shed light on viral tropism and impact across the body. W ... Full text Open Access Link to item Cite

A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2.

Journal Article bioRxiv · February 25, 2021 The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune and cellu ... Full text Link to item Cite

Coupled myovascular expansion directs cardiac growth and regeneration

Journal Article · 2021 ABSTRACTBackground Heart regeneration requires multiple cell types to enable cardiomyocyte (CM) proliferation. How these cells interact to create growth niches is unclear. Here we profile proliferation kinetics of cardiac endothelial cel ... Full text Cite

Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair

Journal Article · 2021 Overwhelming lipid peroxidation induces ferroptotic stress and ferroptosis, a non-apoptotic form of regulated cell death that has been implicated in maladaptive renal repair in mice and humans. Using single-cell transcriptomic and mouse genetic approaches, ... Full text Cite

Identification of a Repair-Supportive Mesenchymal Cell Population during Airway Epithelial Regeneration.

Journal Article Cell Rep · December 22, 2020 Tissue regeneration requires coordinated and dynamic remodeling of stem and progenitor cells and the surrounding niche. Although the plasticity of epithelial cells has been well explored in many tissues, the dynamic changes occurring in niche cells remain ... Full text Link to item Cite

The FDA-approved drug Alectinib compromises SARS-CoV-2 nucleocapsid phosphorylation and inhibits viral infection in vitro.

Journal Article bioRxiv · December 16, 2020 While vaccines are vital for preventing COVID-19 infections, it is critical to develop new therapies to treat patients who become infected. Pharmacological targeting of a host factor required for viral replication can suppress viral spread with a low proba ... Full text Link to item Cite

Longitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19.

Journal Article Immunity · December 15, 2020 Temporal resolution of cellular features associated with a severe COVID-19 disease trajectory is needed for understanding skewed immune responses and defining predictors of outcome. Here, we performed a longitudinal multi-omics study using a two-center coh ... Full text Link to item Cite

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Journal Article Cell Stem Cell · December 3, 2020 Coronavirus infection causes diffuse alveolar damage leading to acute respiratory distress syndrome. The absence of ex vivo models of human alveolar epithelium is hindering an understanding of coronavirus disease 2019 (COVID-19) pathogenesis. Here, we repo ... Full text Open Access Link to item Cite

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.

Journal Article Elife · November 12, 2020 We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral a ... Full text Link to item Cite

BALO: a "mini lung" model to study cell-cell interactions.

Journal Article EMBO J · November 2, 2020 Stem cell-derived organoid models have emerged as a valuable tool for studying organogenesis, cell-to-cell stromal communication and disease. In this issue, Vazquez-Armendariz et al (2020) report a murine lung stem cell-based bronchioalveolar organoid syst ... Full text Link to item Cite

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Journal Article Nat Cell Biol · August 2020 Stem cells undergo dynamic changes in response to injury to regenerate lost cells. However, the identity of transitional states and the mechanisms that drive their trajectories remain understudied. Using lung organoids, multiple in vivo repair models, sing ... Full text Link to item Cite

SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract.

Journal Article Cell · July 23, 2020 The mode of acquisition and causes for the variable clinical spectrum of coronavirus disease 2019 (COVID-19) remain unknown. We utilized a reverse genetics system to generate a GFP reporter virus to explore severe acute respiratory syndrome coronavirus 2 ( ... Full text Link to item Cite

SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues.

Journal Article Cell · May 28, 2020 There is pressing urgency to understand the pathogenesis of the severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2), which causes the disease COVID-19. SARS-CoV-2 spike (S) protein binds angiotensin-converting enzyme 2 (ACE2), and in concert ... Full text Link to item Cite

SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.

Journal Article Nat Med · May 2020 We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and i ... Full text Link to item Cite

Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.

Journal Article Elife · December 12, 2019 Human mutations in the dystroglycan complex (DGC) result in not only muscular dystrophy but also cognitive impairments. However, the molecular architecture critical for the synaptic organization of the DGC in neurons remains elusive. Here, we report Inhibi ... Full text Link to item Cite

Abstract LB-103: Defining oncogenic SWI/SNF complex mediated immune reprogramming in squamous cell carcinoma

Conference Cancer Research · July 1, 2019 AbstractSquamous cell carcinoma (SCC) remains among the most treatment-refractory of human cancers, reflecting in part a limited understanding of the key pathways and mechanisms that drive this disease. The ... Full text Cite

IL-1 and TNFα Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration.

Journal Article Stem Cell Reports · April 9, 2019 Inflammatory responses are known to facilitate tissue recovery following injury. However, the precise mechanisms that enhance lung alveolar regeneration remain unclear. Here, using an organoid-based screening assay, we find that interleukin-1 (IL-1) and tu ... Full text Link to item Cite

ABL kinase inhibition promotes lung regeneration through expansion of an SCGB1A1+ SPC+ cell population following bacterial pneumonia.

Journal Article Proc Natl Acad Sci U S A · January 29, 2019 Current therapeutic interventions for the treatment of respiratory infections are hampered by the evolution of multidrug resistance in pathogens as well as the lack of effective cellular targets. Despite the identification of multiple region-specific lung ... Full text Link to item Cite

Persistence of a novel regeneration-associated transitional cell state in pulmonary fibrosis

Journal Article · 2019 Stem cell senescence is often seen as an age associated pathological state in which cells acquire an abnormal and irreversible state. Here, we show that alveolar stem cell differentiation during lung regeneration involves a unique previously uncharacterize ... Full text Cite

A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.

Journal Article Nature · August 2018 The airways of the lung are the primary sites of disease in asthma and cystic fibrosis. Here we study the cellular composition and hierarchy of the mouse tracheal epithelium by single-cell RNA-sequencing (scRNA-seq) and in vivo lineage tracing. We identify ... Full text Link to item Cite

Pulmonary Neuroendocrine Cells: Sensors and Sentinels of the Lung.

Journal Article Dev Cell · May 21, 2018 Organisms have developed cellular "antennas" to sense, interpret, and integrate environmental stimuli. In a recent issue of Science, Sui et al. (2018) demonstrate that discrete clusters of pulmonary neuroendocrine cells in the lung can sense airborne aller ... Full text Link to item Cite

Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.

Journal Article Cell Stem Cell · May 3, 2018 Cells demonstrate plasticity following injury, but the extent of this phenomenon and the cellular mechanisms involved remain underexplored. Using single-cell RNA sequencing (scRNA-seq) and lineage tracing, we uncover that myoepithelial cells (MECs) of the ... Full text Link to item Cite

A Conserved Distal Lung Regenerative Pathway in Acute Lung Injury.

Journal Article Am J Pathol · May 2018 Improved tools have led to a burgeoning understanding of lung regeneration in mice, but it is not yet known how these insights may be relevant to acute lung injury in humans. We report in detail two cases of fulminant idiopathic acute lung injury requiring ... Full text Link to item Cite

Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.

Journal Article Dev Cell · March 26, 2018 We show that the loss or gain of transcription factor programs that govern embryonic cell-fate specification is associated with a form of tumor plasticity characterized by the acquisition of alternative cell fates normally characteristic of adjacent organs ... Full text Link to item Cite

Plasticity in Olfactory Epithelium: Is It a Sniffer or Shape Shifter?

Journal Article Cell Stem Cell · December 7, 2017 Precise lineage trajectories and the cellular sources that contribute to regeneration after injury are largely unknown in many tissues. In this issue of Cell Stem Cell, Gadye et al. (2017) and Lin et al. (2017) show that olfactory epithelial cells transit ... Full text Link to item Cite

The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis.

Journal Article Dev Biol · April 1, 2017 Wnt proteins are critical for embryonic cardiogenesis and cardiomyogenesis by regulating different intracellular signalling pathways. Whereas canonical Wnt/β-catenin signalling is required for mesoderm induction and proliferation of cardiac progenitor cell ... Full text Open Access Link to item Cite

Plasticity in the lung: making and breaking cell identity.

Journal Article Development · March 1, 2017 In contrast to a prior emphasis on the finality of cell fate decisions in developmental systems, cellular plasticity is now emerging as a general theme in the biology of multiple adult organ systems. In the lung, lineage tracing has been used to identify d ... Full text Open Access Link to item Cite

ACTL6A Is Co-Amplified with p63 in Squamous Cell Carcinoma to Drive YAP Activation, Regenerative Proliferation, and Poor Prognosis.

Journal Article Cancer Cell · January 9, 2017 Loss-of-function mutations in SWI/SNF chromatin-remodeling subunit genes are observed in many cancers, but an oncogenic role for SWI/SNF is not well established. Here, we reveal that ACTL6A, encoding an SWI/SNF subunit linked to stem cell and progenitor ce ... Full text Link to item Cite

Cellular plasticity: 1712 to the present day.

Journal Article Curr Opin Cell Biol · December 2016 Cell identity is a fundamental feature of cells. Tissues are often organized into cellular hierarchies characterized by progressive differentiation and developmental commitment. However, it is been historically evident that the cells of many organisms of v ... Full text Link to item Cite

Regulatory Circuits and Bi-directional Signaling between Stem Cells and Their Progeny.

Journal Article Cell Stem Cell · December 1, 2016 Feedback signals from daughter cells to stem cells are well studied and known to provide important feedback cues. Emerging evidence shows that stem cells also send feedforward signals to their progeny. Complex circuits involving both negative and positive ... Full text Link to item Cite

Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells.

Journal Article Cell Stem Cell · August 4, 2016 Functional modeling of many adult epithelia is limited by the difficulty in maintaining relevant stem cell populations in culture. Here, we show that dual inhibition of SMAD signaling pathways enables robust expansion of primary epithelial basal cell popul ... Full text Link to item Cite

Parent stem cells can serve as niches for their daughter cells.

Journal Article Nature · July 30, 2015 Stem cells integrate inputs from multiple sources. Stem cell niches provide signals that promote stem cell maintenance, while differentiated daughter cells are known to provide feedback signals to regulate stem cell replication and differentiation. Recentl ... Full text Link to item Cite

Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations.

Journal Article Cell Stem Cell · February 5, 2015 Following injury, stem cells restore normal tissue architecture by producing the proper number and proportions of differentiated cells. Current models of airway epithelial regeneration propose that distinct cytokeratin 8-expressing progenitor cells, arisin ... Full text Link to item Cite

TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Journal Article Stem Cell Reports · May 6, 2014 Full text Link to item Cite

Airway-specific inducible transgene expression using aerosolized doxycycline.

Journal Article Am J Respir Cell Mol Biol · December 2013 Tissue-specific transgene expression using tetracycline (tet)-regulated promoter/operator elements has been used to revolutionize our understanding of cellular and molecular processes. However, because most tet-regulated mouse strains use promoters of gene ... Full text Link to item Cite

Dedifferentiation of committed epithelial cells into stem cells in vivo.

Journal Article Nature · November 14, 2013 Cellular plasticity contributes to the regenerative capacity of plants, invertebrates, teleost fishes and amphibians. In vertebrates, differentiated cells are known to revert into replicating progenitors, but these cells do not persist as stable stem cells ... Full text Link to item Cite

Integrated cistromic and expression analysis of amplified NKX2-1 in lung adenocarcinoma identifies LMO3 as a functional transcriptional target.

Journal Article Genes Dev · January 15, 2013 The NKX2-1 transcription factor, a regulator of normal lung development, is the most significantly amplified gene in human lung adenocarcinoma. To study the transcriptional impact of NKX2-1 amplification, we generated an expression signature associated wit ... Full text Link to item Cite

TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Journal Article Stem Cell Reports · 2013 Cell-fate decisions and pluripotency are dependent on networks of key transcriptional regulators. Recent reports demonstrated additional functions of pluripotency-associated factors during early lineage commitment. The T-box transcription factor TBX3 has b ... Full text Link to item Cite

Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC.

Journal Article Nucleic Acids Res · May 2011 The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identific ... Full text Link to item Cite

An updated overview on Wnt signaling pathways: a prelude for more.

Journal Article Circ Res · June 25, 2010 Growth factor signaling is required for cellular differentiation, tissue morphogenesis, and tissue homeostasis. Misregulation of intracellular signal transduction can lead to developmental defects during embryogenesis or particular diseases in the adult. O ... Full text Open Access Link to item Cite