Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.
Publication
, Journal Article
Konishi, S; Tata, A; Tata, PR
Published in: STAR Protoc
June 17, 2022
Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expansion and maintenance of AT2s. The culture conditions are scalable and compatible with high-throughput chemical and genetic screenings and can potentially be used to generate large AT2 numbers for cell-based therapies. For complete details on the use and execution of this protocol, please refer to Katsura et al. (2020).
Duke Scholars
Published In
STAR Protoc
DOI
EISSN
2666-1667
Publication Date
June 17, 2022
Volume
3
Issue
2
Start / End Page
101447
Location
United States
Related Subject Headings
- Stem Cells
- Pulmonary Alveoli
- Mice
- Lung
- Humans
- Cell Differentiation
- Animals
- Alveolar Epithelial Cells
Citation
APA
Chicago
ICMJE
MLA
NLM
Konishi, S., Tata, A., & Tata, P. R. (2022). Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR Protoc, 3(2), 101447. https://doi.org/10.1016/j.xpro.2022.101447
Konishi, Satoshi, Aleksandra Tata, and Purushothama Rao Tata. “Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.” STAR Protoc 3, no. 2 (June 17, 2022): 101447. https://doi.org/10.1016/j.xpro.2022.101447.
Konishi S, Tata A, Tata PR. Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR Protoc. 2022 Jun 17;3(2):101447.
Konishi, Satoshi, et al. “Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.” STAR Protoc, vol. 3, no. 2, June 2022, p. 101447. Pubmed, doi:10.1016/j.xpro.2022.101447.
Konishi S, Tata A, Tata PR. Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR Protoc. 2022 Jun 17;3(2):101447.
Published In
STAR Protoc
DOI
EISSN
2666-1667
Publication Date
June 17, 2022
Volume
3
Issue
2
Start / End Page
101447
Location
United States
Related Subject Headings
- Stem Cells
- Pulmonary Alveoli
- Mice
- Lung
- Humans
- Cell Differentiation
- Animals
- Alveolar Epithelial Cells