Jun Huang

Journal Article Science advances · May 2019 Featured Publication Acoustic tweezers have recently raised great interest across many fields including biology, chemistry, engineering, and medicine, as they can perform contactless, label-free, biocompatible, and precise manipulation of particles and cells. Here, we present ... Full text Cite

Three-dimensional numerical simulation and experimental investigation of boundary-driven streaming in surface acoustic wave microfluidics.

Journal Article Lab on a chip · December 2018 Acoustic streaming has been widely used in microfluidics to manipulate various micro-/nano-objects. In this work, acoustic streaming activated by interdigital transducers (IDT) immersed in highly viscous oil is studied numerically and experimentally. In pa ... Full text Cite

Acoustic tweezers for the life sciences.

Journal Article Nature methods · December 2018 Featured Publication Acoustic tweezers are a versatile set of tools that use sound waves to manipulate bioparticles ranging from nanometer-sized extracellular vesicles to millimeter-sized multicellular organisms. Over the past several decades, the capabilities of acoustic twee ... Full text Cite

High-throughput cell focusing and separation via acoustofluidic tweezers.

Journal Article Lab on a chip · September 2018 Separation of particles and cells is an important function in many biological and biomedical protocols. Although a variety of microfluidic-based techniques have been developed so far, there is clearly still a demand for a precise, fast, and biocompatible m ... Full text Cite

Digital acoustofluidics enables contactless and programmable liquid handling.

Journal Article Nature communications · July 2018 Featured Publication For decades, scientists have pursued the goal of performing automated reactions in a compact fluid processor with minimal human intervention. Most advanced fluidic handling technologies (e.g., microfluidic chips and micro-well plates) lack fluid rewritabil ... Full text Cite

A sharp-edge-based acoustofluidic chemical signal generator.

Journal Article Lab on a chip · May 2018 Resolving the temporal dynamics of cell signaling pathways is essential for regulating numerous downstream functions, from gene expression to cellular responses. Mapping these signaling pathways requires the exposure of cells to time-varying chemical signa ... Full text Cite

Clinical utility of non-EpCAM based circulating tumor cell assays.

Journal Article Adv Drug Deliv Rev · February 1, 2018 Methods enabling the isolation, detection, and characterization of circulating tumor cells (CTCs) in blood have clear potential to facilitate precision medicine approaches in patients with cancer, not only for prognostic purposes but also for prediction of ... Full text Link to item Cite

Acoustofluidic devices controlled by cell phones.

Journal Article Lab on a chip · January 2018 Acoustofluidic devices have continuously demonstrated their potential to impact medical diagnostics and lab-on-a-chip applications. To bring these technologies to real-world applications, they must be made more accessible to end users. Herein, we report on ... Full text Cite

Isolation of exosomes from whole blood by integrating acoustics and microfluidics.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · October 2017 Featured Publication Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significa ... Full text Cite

Acoustofluidic coating of particles and cells.

Journal Article Lab on a chip · November 2016 On-chip microparticle and cell coating technologies enable a myriad of applications in chemistry, engineering, and medicine. Current microfluidic coating technologies often rely on magnetic labeling and concurrent deflection of particles across laminar str ... Full text Cite

Acoustofluidic Rotational Manipulation of Cells and Organisms Using Oscillating Solid Structures.

Journal Article Small (Weinheim an der Bergstrasse, Germany) · October 2016 A polydimethylsiloxane microchannel featuring sidewall sharp-edge structures and bare channels, and a piezoelement transducer is attached to a thin glass slide. When an external acoustic field is applied to the microchannel, the oscillation of the sharp-ed ... Full text Cite

High-throughput acoustic separation of platelets from whole blood.

Journal Article Lab on a chip · September 2016 Featured Publication Platelets contain growth factors which are important in biomedical and clinical applications. In this work, we present an acoustic separation device for high-throughput, non-invasive platelet isolation. In particular, we separated platelets from whole bloo ... Full text Cite

Rotational manipulation of single cells and organisms using acoustic waves

Journal Article Nature Communications · March 23, 2016 Featured Publication AbstractThe precise rotational manipulation of single cells or organisms is invaluable to many applications in biology, chemistry, physics and medicine. In this article, we describe an acoustic-based, on-chip manipulation m ... Full text Cite

Three-dimensional manipulation of single cells using surface acoustic waves

Journal Article Proceedings of the National Academy of Sciences · February 9, 2016 Featured Publication SignificanceWe present 3D acoustic tweezers, which can trap and manipulate single cells and particles along three mutually orthogonal axes of motion by recourse to surface acoustic waves. We use 3D acoustic tweez ... Full text Cite

Acoustic separation of circulating tumor cells.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · April 2015 Featured Publication Circulating tumor cells (CTCs) are important targets for cancer biology studies. To further elucidate the role of CTCs in cancer metastasis and prognosis, effective methods for isolating extremely rare tumor cells from peripheral blood must be developed. A ... Full text Cite

Controlling cell–cell interactions using surface acoustic waves

Journal Article Proceedings of the National Academy of Sciences · January 6, 2015 Featured Publication SignificanceWe present a unique acoustic well approach that can precisely control cell-to-cell distance and cell–cell interactions. Our technology can achieve high precision and high throughput simultaneously whi ... Full text Cite

A reliable and programmable acoustofluidic pump powered by oscillating sharp-edge structures.

Journal Article Lab on a chip · November 2014 Featured Publication We present a programmable acoustofluidic pump that utilizes the acoustic streaming effects generated by the oscillation of tilted sharp-edge structures. This sharp-edge-based acoustofluidic pump is capable of generating stable flow rates as high as 8 μL mi ... Full text Cite

An acoustofluidic micromixer based on oscillating sidewall sharp-edges.

Journal Article Lab on a chip · October 2013 Featured Publication Rapid and homogeneous mixing inside a microfluidic channel is demonstrated via the acoustic streaming phenomenon induced by the oscillation of sidewall sharp-edges. By optimizing the design of the sharp-edges, excellent mixing performance and fast mixing s ... Full text Cite

Surface acoustic wave microfluidics

Journal Article Lab on a Chip · 2013 Featured Publication Full text Cite

On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves

Journal Article Proceedings of the National Academy of Sciences · July 10, 2012 Featured Publication Techniques that can dexterously manipulate single particles, cells, and organisms are invaluable for many applications in biology, chemistry, engineering, and physics. Here, we demonstrate standing surface acoustic wave based “ ... Full text Cite