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Tyler Allen

Research Program Leader, Tier 1
Duke Cancer Institute
Box 103861, 3044 GSRB 1, Durham, NC 27710
905 LaSalle Street, 6044 GSRB I, Durham, NC 27704

Selected Publications

Characterization of a castrate-resistant prostate cancer xenograft derived from a patient of West African ancestry.

Conference Prostate Cancer Prostatic Dis · September 2022 BACKGROUND: Prostate cancer is a clinically and molecularly heterogeneous disease, with highest incidence and mortality among men of African ancestry. To date, prostate cancer patient-derived xenograft (PCPDX) models to study this disease have been difficu ... Full text Open Access Link to item Cite

RNA splicing and aggregate gene expression differences in lung squamous cell carcinoma between patients of West African and European ancestry.

Journal Article Lung Cancer · March 2021 OBJECTIVES: Despite disparities in lung cancer incidence and mortality, the molecular landscape of lung cancer in patients of African ancestry remains underexplored, and race-related differences in RNA splicing remain unexplored. MATERIALS AND METHODS: We ... Full text Link to item Cite

Biological Aspects of Cancer Health Disparities.

Book · January 27, 2021 Racial and ethnic disparities span the continuum of cancer care and are driven by a complex interplay among social, psychosocial, lifestyle, environmental, health system, and biological determinants of health. Research is needed to identify these determina ... Full text Link to item Cite

Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis.

Chapter · 2021 Cancer mortality rates are primarily a result of cancer metastasis. Recent advances in microscopy technology allow for the imaging of circulating tumor cells (CTCs) as they extravasate (exit) blood vessels, a key step in the metastasis process. Here, we de ... Full text Link to item Cite

A Zebrafish Model of Metastatic Colonization Pinpoints Cellular Mechanisms of Circulating Tumor Cell Extravasation.

Journal Article Frontiers in oncology · January 2021 Metastasis is a multistep process in which cells must detach, migrate/invade local structures, intravasate, circulate, extravasate, and colonize. A full understanding of the complexity of this process has been limited by the lack of ability to study these ... Full text Cite

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Journal Article Nature communications · February 2020 Idiopathic pulmonary fibrosis (IPF) is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation. As fibrosis thickens, the lung tissue loses the ability to facilitate gas exchange and provide cells ... Full text Cite

Tumor cell-derived exosomes home to their cells of origin and can be used as Trojan horses to deliver cancer drugs.

Journal Article Theranostics · 2020 Cancer is the second leading cause of death worldwide and patients are in urgent need of therapies that can effectively target cancer with minimal off-target side effects. Exosomes are extracellular nano-shuttles that facilitate intercellular communication ... Full text Open Access Link to item Cite

Circulating tumor cells exit circulation while maintaining multicellularity, augmenting metastatic potential.

Journal Article J Cell Sci · September 9, 2019 Metastasis accounts for the majority of all cancer deaths, yet the process remains poorly understood. A pivotal step in the metastasis process is the exiting of tumor cells from the circulation, a process known as extravasation. However, it is unclear how ... Full text Open Access Link to item Cite

microRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential.

Journal Article J Clin Invest · April 29, 2019 Exosomes, as functional paracrine units of therapeutic cells, can partially reproduce the reparative properties of their parental cells. The constitution of exosomes, as well as their biological activity, largely depends on the cells that secrete them. We ... Full text Open Access Link to item Cite

Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury.

Journal Article Adv Funct Mater · January 24, 2019 Cardiovascular disease is the leading cause of mortality worldwide. While reperfusion therapy is vital for patient survival post-heart attack, it also causes further tissue injury, known as myocardial ischemia/reperfusion (I/R) injury in clinical practice. ... Full text Open Access Link to item Cite

Cardiac Stem Cell Patch Integrated with Microengineered Blood Vessels Promotes Cardiomyocyte Proliferation and Neovascularization after Acute Myocardial Infarction.

Journal Article ACS Appl Mater Interfaces · October 3, 2018 Cardiac stem cell (CSC) therapy has shown preclinical and clinical evidence for ischemic heart repair but is limited by low cellular engraftment and survival after transplantation. Previous versions of the cardiac patch strategy improve stem cell engraftme ... Full text Open Access Link to item Cite

Mesenchymal Stem Cell/Red Blood Cell-Inspired Nanoparticle Therapy in Mice with Carbon Tetrachloride-Induced Acute Liver Failure.

Journal Article ACS Nano · July 24, 2018 Acute liver failure is a critical condition characterized by global hepatocyte death and often time needs a liver transplantation. Such treatment is largely limited by donor organ shortage. Stem cell therapy offers a promising option to patients with acute ... Full text Open Access Link to item Cite

Targeted repair of heart injury by stem cells fused with platelet nanovesicles.

Journal Article Nat Biomed Eng · 2018 Stem cell transplantation, as used clinically, suffers from low retention and engraftment of the transplanted cells. Inspired by the ability of platelets to recruit stem cells to sites of injury on blood vessels, we hypothesized that platelets might enhanc ... Full text Open Access Link to item Cite

Safety and Efficacy of Allogeneic Lung Spheroid Cells in a Mismatched Rat Model of Pulmonary Fibrosis.

Journal Article Stem cells translational medicine · October 2017 Idiopathic pulmonary fibrosis is a devastating interstitial lung disease characterized by the relentless deposition of extracellular matrix causing lung distortions and dysfunctions. The prognosis after detection is merely 3-5 years and the only two Food a ... Full text Cite

Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies.

Journal Article Respir Res · June 30, 2017 BACKGROUND: Resident stem and progenitor cells have been identified in the lung over the last decade, but isolation and culture of these cells remains a challenge. Thus, although these lung stem and progenitor cells provide an ideal source for stem-cell ba ... Full text Link to item Cite

A Regenerative Cardiac Patch Formed by Spray Painting of Biomaterials onto the Heart.

Journal Article Tissue Eng Part C Methods · March 2017 Layering a regenerative polymer scaffold on the surface of the heart, termed as a cardiac patch, has been proven to be effective in preserving cardiac function after myocardial infarction (MI). However, the placement of such a patch on the heart usually ne ... Full text Link to item Cite

Angiopellosis as an Alternative Mechanism of Cell Extravasation.

Journal Article Stem cells (Dayton, Ohio) · January 2017 Stem cells possess the ability to home in and travel to damaged tissue when injected intravenously. For the cells to exert their therapeutic effect, they must cross the blood vessel wall and enter the surrounding tissues. The mechanism of extravasation inj ... Full text Cite

Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome.

Journal Article Nature communications · January 2017 Stem cell therapy represents a promising strategy in regenerative medicine. However, cells need to be carefully preserved and processed before usage. In addition, cell transplantation carries immunogenicity and/or tumourigenicity risks. Mounting lines of e ... Full text Cite

Adult Lung Spheroid Cells Contain Progenitor Cells and Mediate Regeneration in Rodents With Bleomycin-Induced Pulmonary Fibrosis.

Journal Article Stem cells translational medicine · November 2015 UnlabelledLung diseases are devastating conditions and ranked as one of the top five causes of mortality worldwide according to the World Health Organization. Stem cell therapy is a promising strategy for lung regeneration. Previous animal and cli ... Full text Cite

Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips.

Journal Article PLoS One · 2015 AIMS: The coronary artery ligation model in rodents mimics human myocardial infarction (MI). Normally mechanical ventilation and prolonged anesthesia period are needed. Recently, a method has been developed to create MI by popping-out the heart (without ve ... Full text Link to item Cite