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Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.

Publication ,  Journal Article
Juhas, M; Engelmayr, GC; Fontanella, AN; Palmer, GM; Bursac, N
Published in: Proc Natl Acad Sci U S A
April 15, 2014

Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady ingrowth of blood-perfused microvasculature along with an increase in amplitude of calcium transients and force of contraction. We also demonstrated superior structural organization, vascularization, and contractile function of fully differentiated vs. undifferentiated engineered muscle implants. The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

April 15, 2014

Volume

111

Issue

15

Start / End Page

5508 / 5513

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Muscle, Skeletal
  • Muscle Development
  • Muscle Contraction
  • Microvessels
  • Mice, Nude
  • Mice
  • Cobra Cardiotoxin Proteins
  • Biomimetics
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Juhas, M., Engelmayr, G. C., Fontanella, A. N., Palmer, G. M., & Bursac, N. (2014). Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo. Proc Natl Acad Sci U S A, 111(15), 5508–5513. https://doi.org/10.1073/pnas.1402723111
Juhas, Mark, George C. Engelmayr, Andrew N. Fontanella, Gregory M. Palmer, and Nenad Bursac. “Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.Proc Natl Acad Sci U S A 111, no. 15 (April 15, 2014): 5508–13. https://doi.org/10.1073/pnas.1402723111.
Juhas M, Engelmayr GC, Fontanella AN, Palmer GM, Bursac N. Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5508–13.
Juhas, Mark, et al. “Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.Proc Natl Acad Sci U S A, vol. 111, no. 15, Apr. 2014, pp. 5508–13. Pubmed, doi:10.1073/pnas.1402723111.
Juhas M, Engelmayr GC, Fontanella AN, Palmer GM, Bursac N. Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5508–5513.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

April 15, 2014

Volume

111

Issue

15

Start / End Page

5508 / 5513

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Muscle, Skeletal
  • Muscle Development
  • Muscle Contraction
  • Microvessels
  • Mice, Nude
  • Mice
  • Cobra Cardiotoxin Proteins
  • Biomimetics
  • Animals