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DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.

Publication ,  Journal Article
Kulkarni, V; Rosati, M; Jalah, R; Ganneru, B; Alicea, C; Yu, L; Guan, Y; LaBranche, C; Montefiori, DC; King, AD; Valentin, A; Pavlakis, GN; Felber, BK
Published in: J Med Primatol
October 2014

BACKGROUND: A desirable HIV vaccine should induce protective long-lasting humoral and cellular immune responses. METHODS: Macaques were immunized by env DNA, selected from a panel of recently transmitted SIVmac251 Env using intradermal electroporation as vaccine delivery method and magnitude, breadth and longevity of humoral and cellular immune responses. RESULTS: The macaques developed high, long-lasting humoral immune responses with neutralizing capacity against homologous and heterologous Env. The avidity of the antibody responses was also preserved over 1-year follow-up. Analysis of cellular immune responses demonstrated induction of Env-specific memory T cells harboring granzyme B, albeit their overall levels were low. Similar to the humoral responses, the cellular immunity was persistent over the ~1-year follow-up. CONCLUSION: These data show that vaccination by this intradermal DNA delivery regimen is able to induce potent and durable immune responses in macaques.

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Published In

J Med Primatol

DOI

EISSN

1600-0684

Publication Date

October 2014

Volume

43

Issue

5

Start / End Page

329 / 340

Location

Denmark

Related Subject Headings

  • Virology
  • Vaccination
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
  • Injections, Intradermal
 

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Kulkarni, V., Rosati, M., Jalah, R., Ganneru, B., Alicea, C., Yu, L., … Felber, B. K. (2014). DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques. J Med Primatol, 43(5), 329–340. https://doi.org/10.1111/jmp.12123
Kulkarni, Viraj, Margherita Rosati, Rashmi Jalah, Brunda Ganneru, Candido Alicea, Lei Yu, Yongjun Guan, et al. “DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.J Med Primatol 43, no. 5 (October 2014): 329–40. https://doi.org/10.1111/jmp.12123.
Kulkarni V, Rosati M, Jalah R, Ganneru B, Alicea C, Yu L, et al. DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques. J Med Primatol. 2014 Oct;43(5):329–40.
Kulkarni, Viraj, et al. “DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.J Med Primatol, vol. 43, no. 5, Oct. 2014, pp. 329–40. Pubmed, doi:10.1111/jmp.12123.
Kulkarni V, Rosati M, Jalah R, Ganneru B, Alicea C, Yu L, Guan Y, LaBranche C, Montefiori DC, King AD, Valentin A, Pavlakis GN, Felber BK. DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques. J Med Primatol. 2014 Oct;43(5):329–340.
Journal cover image

Published In

J Med Primatol

DOI

EISSN

1600-0684

Publication Date

October 2014

Volume

43

Issue

5

Start / End Page

329 / 340

Location

Denmark

Related Subject Headings

  • Virology
  • Vaccination
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
  • Injections, Intradermal