Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio).

Publication ,  Journal Article
Fang, M; Getzinger, GJ; Cooper, EM; Clark, BW; Garner, LVT; Di Giulio, RT; Ferguson, PL; Stapleton, HM
Published in: Environmental toxicology and chemistry
December 2014

In the present study, effect-directed analysis was used to identify teratogenic compounds in porewater collected from a Superfund site along the Elizabeth River estuary (VA, USA). Zebrafish (Danio rerio) exposed to the porewater displayed acute developmental toxicity and cardiac teratogenesis, presumably because of elevated sediment levels of polycyclic aromatic hydrocarbons (PAHs) from historical creosote use. Pretreatment of porewater with several physical and chemical particle removal methods revealed that colloid-bound chemicals constituted the bulk of the observed toxicity. Size-exclusive chromatography and normal-phase high-performance liquid chromatography were used to fractionate Elizabeth River porewater. Acute toxicity of porewater extracts and extract fractions was assessed as the pericardial area in embryonic zebrafish. The most toxic fraction contained several known aryl hydrocarbon receptor (AhR) agonists (e.g., 1,2-benzofluorene and 1,2-benzanthracene) and cytochrome P450 A1 (CPY1A) inhibitors (e.g., dibenzothiophene and fluoranthene). The second most toxic fraction contained known AhR agonists (e.g., benzo[a]pyrene and indeno[1,2,3-cd]pyrene). Addition of a CYP1A inhibitor, fluoranthene, increased toxicity in all active porewater fractions, suggesting synergism between several contaminants present in porewaters. The results indicate that the observed acute toxicity associated with Elizabeth River porewater results from high concentrations of AhR agonistic PAHs and mixture effects related to interactions between compounds co-occurring at the Elizabeth River site. However, even after extensive fractionation and chemical characterization, it remains plausible that some active compounds in Elizabeth River porewater remain unidentified.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Environmental toxicology and chemistry

DOI

EISSN

1552-8618

ISSN

0730-7268

Publication Date

December 2014

Volume

33

Issue

12

Start / End Page

2767 / 2774

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Virginia
  • Rivers
  • Receptors, Aryl Hydrocarbon
  • Pyrenes
  • Polycyclic Aromatic Hydrocarbons
  • Fluorenes
  • Environmental Sciences
  • Embryonic Development
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fang, M., Getzinger, G. J., Cooper, E. M., Clark, B. W., Garner, L. V. T., Di Giulio, R. T., … Stapleton, H. M. (2014). Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio). Environmental Toxicology and Chemistry, 33(12), 2767–2774. https://doi.org/10.1002/etc.2738
Fang, Mingliang, Gordon J. Getzinger, Ellen M. Cooper, Bryan W. Clark, Lindsey V. T. Garner, Richard T. Di Giulio, P Lee Ferguson, and Heather M. Stapleton. “Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio).Environmental Toxicology and Chemistry 33, no. 12 (December 2014): 2767–74. https://doi.org/10.1002/etc.2738.
Fang M, Getzinger GJ, Cooper EM, Clark BW, Garner LVT, Di Giulio RT, et al. Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio). Environmental toxicology and chemistry. 2014 Dec;33(12):2767–74.
Fang, Mingliang, et al. “Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio).Environmental Toxicology and Chemistry, vol. 33, no. 12, Dec. 2014, pp. 2767–74. Epmc, doi:10.1002/etc.2738.
Fang M, Getzinger GJ, Cooper EM, Clark BW, Garner LVT, Di Giulio RT, Ferguson PL, Stapleton HM. Effect-directed analysis of Elizabeth River porewater: developmental toxicity in zebrafish (Danio rerio). Environmental toxicology and chemistry. 2014 Dec;33(12):2767–2774.
Journal cover image

Published In

Environmental toxicology and chemistry

DOI

EISSN

1552-8618

ISSN

0730-7268

Publication Date

December 2014

Volume

33

Issue

12

Start / End Page

2767 / 2774

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Virginia
  • Rivers
  • Receptors, Aryl Hydrocarbon
  • Pyrenes
  • Polycyclic Aromatic Hydrocarbons
  • Fluorenes
  • Environmental Sciences
  • Embryonic Development