Skip to main content
release_alert
Welcome to the new Scholars 3.0! Read about new features and let us know what you think.
cancel

Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation.

Publication ,  Journal Article
Kwun, J; Malarkannan, S; Burlingham, WJ; Knechtle, SJ
Published in: Journal of immunology (Baltimore, Md. : 1950)
October 2011

Grafts can be rejected even when matched for MHC because of differences in the minor histocompatibility Ags (mH-Ags). H4- and H60-derived epitopes are known as immunodominant mH-Ags in H2(b)-compatible BALB.B to C57BL/6 transplantation settings. Although multiple explanations have been provided to explain immunodominance of Ags, the role of vascularization of the graft is yet to be determined. In this study, we used heart (vascularized) and skin (nonvascularized) transplantations to determine the role of primary vascularization of the graft. A higher IFN-γ response toward H60 peptide occurs in heart recipients. In contrast, a higher IFN-γ response was generated against H4 peptide in skin transplant recipients. Peptide-loaded tetramer staining revealed a distinct antigenic hierarchy between heart and skin transplantation: H60-specific CD8(+) T cells were the most abundant after heart transplantation, whereas H4-specific CD8(+) T cells were more abundant after skin graft. Neither the tissue-specific distribution of mH-Ags nor the draining lymph node-derived dendritic cells correlated with the observed immunodominance. Interestingly, non-primarily vascularized cardiac allografts mimicked skin grafts in the observed immunodominance, and H60 immunodominance was observed in primarily vascularized skin grafts. However, T cell depletion from the BALB.B donor prior to cardiac allograft induces H4 immunodominance in vascularized cardiac allograft. Collectively, our data suggest that immediate transmigration of donor T cells via primary vascularization is responsible for the immunodominance of H60 mH-Ag in organ and tissue transplantation.

Duke Scholars

Published In

Journal of immunology (Baltimore, Md. : 1950)

DOI

EISSN

1550-6606

ISSN

0022-1767

Publication Date

October 2011

Volume

187

Issue

8

Start / End Page

3997 / 4006

Related Subject Headings

  • Transplantation, Homologous
  • Skin Transplantation
  • Neovascularization, Physiologic
  • Minor Histocompatibility Antigens
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Activation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kwun, J., Malarkannan, S., Burlingham, W. J., & Knechtle, S. J. (2011). Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation. Journal of Immunology (Baltimore, Md. : 1950), 187(8), 3997–4006. https://doi.org/10.4049/jimmunol.1003918
Kwun, Jean, Subramaniam Malarkannan, William J. Burlingham, and Stuart J. Knechtle. “Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation.Journal of Immunology (Baltimore, Md. : 1950) 187, no. 8 (October 2011): 3997–4006. https://doi.org/10.4049/jimmunol.1003918.
Kwun J, Malarkannan S, Burlingham WJ, Knechtle SJ. Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation. Journal of immunology (Baltimore, Md : 1950). 2011 Oct;187(8):3997–4006.
Kwun, Jean, et al. “Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation.Journal of Immunology (Baltimore, Md. : 1950), vol. 187, no. 8, Oct. 2011, pp. 3997–4006. Epmc, doi:10.4049/jimmunol.1003918.
Kwun J, Malarkannan S, Burlingham WJ, Knechtle SJ. Primary vascularization of the graft determines the immunodominance of murine minor H antigens during organ transplantation. Journal of immunology (Baltimore, Md : 1950). 2011 Oct;187(8):3997–4006.

Published In

Journal of immunology (Baltimore, Md. : 1950)

DOI

EISSN

1550-6606

ISSN

0022-1767

Publication Date

October 2011

Volume

187

Issue

8

Start / End Page

3997 / 4006

Related Subject Headings

  • Transplantation, Homologous
  • Skin Transplantation
  • Neovascularization, Physiologic
  • Minor Histocompatibility Antigens
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Activation