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A clinically relevant model of perinatal global ischemic brain damage in rats.

Publication ,  Journal Article
Yang, T; Zhuang, L; Terrando, N; Wu, X; Jonhson, MR; Maze, M; Ma, D
Published in: Brain Res
April 6, 2011

We have designed a clinically relevant model of perinatal asphyxia providing intrapartum hypoxia in rats. On gestation day 22 SD rats were anesthetized and the uterine horns were exteriorized and placed in a water bath at 37°C for up to 20min. After this, pups were delivered from the uterus and manually stimulated to initiate breathing in an incubator at 37°C for 1 h in air. Brains were harvested and stained with cresyl violet, caspase-3, and TUNEL to detect morphological and apoptotic changes on postnatal days (PND) 1, 3, and 7. Separate cohorts were maintained until PND 50 and tested for learning and memory using Morris water maze (WM). Survival rate was decreased with longer hypoxic time, and 100% mortality was noted when hypoxia time was beyond 18min. Apoptosis was increased with the duration of hypoxia with neuronal loss and cell shrinkage in the CA1 of hippocampus. The time taken for the juveniles to locate the hidden platform during WM was increased in animals subjected to hypoxia. These data demonstrate that perinatal ischemic injury leads to neuronal death in the hippocampus and long-lasting cognitive dysfunction. This model mimics hypoxic ischemic encephalopathy in humans and may be appropriate for investigating therapeutic interventions.

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Published In

Brain Res

DOI

EISSN

1872-6240

Publication Date

April 6, 2011

Volume

1383

Start / End Page

317 / 323

Location

Netherlands

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Neurology & Neurosurgery
  • Maze Learning
  • Infant, Newborn
  • In Situ Nick-End Labeling
  • Immunohistochemistry
  • Hypoxia-Ischemia, Brain
  • Humans
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
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Yang, T., Zhuang, L., Terrando, N., Wu, X., Jonhson, M. R., Maze, M., & Ma, D. (2011). A clinically relevant model of perinatal global ischemic brain damage in rats. Brain Res, 1383, 317–323. https://doi.org/10.1016/j.brainres.2011.01.081
Yang, Ting, Lei Zhuang, Niccolò Terrando, Xinmin Wu, Mark R. Jonhson, Mervyn Maze, and Daqing Ma. “A clinically relevant model of perinatal global ischemic brain damage in rats.Brain Res 1383 (April 6, 2011): 317–23. https://doi.org/10.1016/j.brainres.2011.01.081.
Yang T, Zhuang L, Terrando N, Wu X, Jonhson MR, Maze M, et al. A clinically relevant model of perinatal global ischemic brain damage in rats. Brain Res. 2011 Apr 6;1383:317–23.
Yang, Ting, et al. “A clinically relevant model of perinatal global ischemic brain damage in rats.Brain Res, vol. 1383, Apr. 2011, pp. 317–23. Pubmed, doi:10.1016/j.brainres.2011.01.081.
Yang T, Zhuang L, Terrando N, Wu X, Jonhson MR, Maze M, Ma D. A clinically relevant model of perinatal global ischemic brain damage in rats. Brain Res. 2011 Apr 6;1383:317–323.
Journal cover image

Published In

Brain Res

DOI

EISSN

1872-6240

Publication Date

April 6, 2011

Volume

1383

Start / End Page

317 / 323

Location

Netherlands

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Neurology & Neurosurgery
  • Maze Learning
  • Infant, Newborn
  • In Situ Nick-End Labeling
  • Immunohistochemistry
  • Hypoxia-Ischemia, Brain
  • Humans
  • Disease Models, Animal