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λ Light Chain Bias Associated With Enhanced Binding and Function of Anti-HIV Env Glycoprotein Antibodies.

Publication ,  Journal Article
Sajadi, MM; Farshidpour, M; Brown, EP; Ouyang, X; Seaman, MS; Pazgier, M; Ackerman, ME; Robinson, H; Tomaras, G; Parsons, MS; Charurat, M ...
Published in: J Infect Dis
January 1, 2016

The humoral response to human immunodeficiency virus (HIV) remains incompletely understood. In this report, we describe biased λ light chain use during the HIV Env glycoprotein (Env) response in HIV infection and vaccination. We examined HIV Env binding (and neutralization) in the context of light chain use in subjects with acute HIV infection, chronic HIV infection, and among HIV vaccinees. In all populations tested, there was a λ chain bias for HIV Env binding antibodies, compared with other HIV antigens (such as p24) or tetanus toxoid. In subjects with chronic HIV infection, a λ bias was noted for neutralization, with λ antibodies accounting for up to 90% of all neutralization activity observed. This is the first report of antibody function in a human infection being tied to light chain use. In HIV infection, antibodies expressing λ light chains tended to have longer CDRL3s, increased light chain contact with HIV Env, and less hypermutation in the heavy chain, compared with antibodies using the κ light chain. These data also support an evolutionary model for the understanding the various κ to λ light chain ratios observed across species and suggest that the λ light chain bias against HIV provides the host an advantage in developing a more efficient humoral response.

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Published In

J Infect Dis

DOI

EISSN

1537-6613

Publication Date

January 1, 2016

Volume

213

Issue

1

Start / End Page

156 / 164

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Young Adult
  • Protein Binding
  • Middle Aged
  • Microbiology
  • Male
  • Immunoglobulin Light Chains
  • Immunity, Humoral
  • Humans
  • HIV-1
 

Citation

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Sajadi, M. M., Farshidpour, M., Brown, E. P., Ouyang, X., Seaman, M. S., Pazgier, M., … Lewis, G. K. (2016). λ Light Chain Bias Associated With Enhanced Binding and Function of Anti-HIV Env Glycoprotein Antibodies. J Infect Dis, 213(1), 156–164. https://doi.org/10.1093/infdis/jiv448
Sajadi, Mohammad M., Maham Farshidpour, Eric P. Brown, Xin Ouyang, Michael S. Seaman, Marzena Pazgier, Margaret E. Ackerman, et al. “λ Light Chain Bias Associated With Enhanced Binding and Function of Anti-HIV Env Glycoprotein Antibodies.J Infect Dis 213, no. 1 (January 1, 2016): 156–64. https://doi.org/10.1093/infdis/jiv448.
Sajadi MM, Farshidpour M, Brown EP, Ouyang X, Seaman MS, Pazgier M, et al. λ Light Chain Bias Associated With Enhanced Binding and Function of Anti-HIV Env Glycoprotein Antibodies. J Infect Dis. 2016 Jan 1;213(1):156–64.
Sajadi, Mohammad M., et al. “λ Light Chain Bias Associated With Enhanced Binding and Function of Anti-HIV Env Glycoprotein Antibodies.J Infect Dis, vol. 213, no. 1, Jan. 2016, pp. 156–64. Pubmed, doi:10.1093/infdis/jiv448.
Sajadi MM, Farshidpour M, Brown EP, Ouyang X, Seaman MS, Pazgier M, Ackerman ME, Robinson H, Tomaras G, Parsons MS, Charurat M, DeVico AL, Redfield RR, Lewis GK. λ Light Chain Bias Associated With Enhanced Binding and Function of Anti-HIV Env Glycoprotein Antibodies. J Infect Dis. 2016 Jan 1;213(1):156–164.
Journal cover image

Published In

J Infect Dis

DOI

EISSN

1537-6613

Publication Date

January 1, 2016

Volume

213

Issue

1

Start / End Page

156 / 164

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Young Adult
  • Protein Binding
  • Middle Aged
  • Microbiology
  • Male
  • Immunoglobulin Light Chains
  • Immunity, Humoral
  • Humans
  • HIV-1