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Invited Review Article: Pump-probe microscopy

Publication ,  Journal Article
Fischer, MC; Wilson, JW; Robles, FE; Warren, WS
Published in: Review of Scientific Instruments
March 1, 2016

Multiphoton microscopy has rapidly gained popularity in biomedical imaging and materials science because of its ability to provide three-dimensional images at high spatial and temporal resolution even in optically scattering environments. Currently the majority of commercial and home-built devices are based on two-photon fluorescence and harmonic generation contrast. These two contrast mechanisms are relatively easy to measure but can access only a limited range of endogenous targets. Recent developments in fast laser pulse generation, pulse shaping, and detection technology have made accessible a wide range of optical contrasts that utilize multiple pulses of different colors. Molecular excitation with multiple pulses offers a large number of adjustable parameters. For example, in two-pulse pump-probe microscopy, one can vary the wavelength of each excitation pulse, the detection wavelength, the timing between the excitation pulses, and the detection gating window after excitation. Such a large parameter space can provide much greater molecular specificity than existing single-color techniques and allow for structural and functional imaging without the need for exogenous dyes and labels, which might interfere with the system under study. In this review, we provide a tutorial overview, covering principles of pump-probe microscopy and experimental setup, challenges associated with signal detection and data processing, and an overview of applications.

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Published In

Review of Scientific Instruments

DOI

EISSN

1089-7623

ISSN

0034-6748

Publication Date

March 1, 2016

Volume

87

Issue

3

Publisher

AIP Publishing

Related Subject Headings

  • Photons
  • Optical Phenomena
  • Microscopy
  • Image Processing, Computer-Assisted
  • Humans
  • Applied Physics
  • Animals
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

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ICMJE
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Fischer, M. C., Wilson, J. W., Robles, F. E., & Warren, W. S. (2016). Invited Review Article: Pump-probe microscopy. Review of Scientific Instruments, 87(3). https://doi.org/10.1063/1.4943211
Fischer, Martin C., Jesse W. Wilson, Francisco E. Robles, and Warren S. Warren. “Invited Review Article: Pump-probe microscopy.” Review of Scientific Instruments 87, no. 3 (March 1, 2016). https://doi.org/10.1063/1.4943211.
Fischer MC, Wilson JW, Robles FE, Warren WS. Invited Review Article: Pump-probe microscopy. Review of Scientific Instruments. 2016 Mar 1;87(3).
Fischer, Martin C., et al. “Invited Review Article: Pump-probe microscopy.” Review of Scientific Instruments, vol. 87, no. 3, AIP Publishing, Mar. 2016. Crossref, doi:10.1063/1.4943211.
Fischer MC, Wilson JW, Robles FE, Warren WS. Invited Review Article: Pump-probe microscopy. Review of Scientific Instruments. AIP Publishing; 2016 Mar 1;87(3).

Published In

Review of Scientific Instruments

DOI

EISSN

1089-7623

ISSN

0034-6748

Publication Date

March 1, 2016

Volume

87

Issue

3

Publisher

AIP Publishing

Related Subject Headings

  • Photons
  • Optical Phenomena
  • Microscopy
  • Image Processing, Computer-Assisted
  • Humans
  • Applied Physics
  • Animals
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences