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Efficient Culture of Human Naive and Memory B Cells for Use as APCs.

Publication ,  Journal Article
Su, K-Y; Watanabe, A; Yeh, C-H; Kelsoe, G; Kuraoka, M
Published in: J Immunol
November 15, 2016

The ability to culture and expand B cells in vitro has become a useful tool for studying human immunity. A limitation of current methods for human B cell culture is the capacity to support mature B cell proliferation. We developed a culture method to support the efficient activation and proliferation of naive and memory human B cells. This culture supports extensive B cell proliferation, with ∼103-fold increases following 8 d in culture and 106-fold increases when cultures are split and cultured for 8 more days. In culture, a significant fraction of naive B cells undergo isotype switching and differentiate into plasmacytes. Culture-derived (CD) B cells are readily cryopreserved and, when recovered, retain their ability to proliferate and differentiate. Significantly, proliferating CD B cells express high levels of MHC class II, CD80, and CD86. CD B cells act as APCs and present alloantigens and microbial Ags to T cells. We are able to activate and expand Ag-specific memory B cells; these cultured cells are highly effective in presenting Ag to T cells. We characterized the TCR repertoire of rare Ag-specific CD4+ T cells that proliferated in response to tetanus toxoid (TT) presented by autologous CD B cells. TCR Vβ usage by TT-activated CD4+ T cells differs from resting and unspecifically activated CD4+ T cells. Moreover, we found that TT-specific TCR Vβ usage by CD4+ T cells was substantially different between donors. This culture method provides a platform for studying the BCR and TCR repertoires within a single individual.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

November 15, 2016

Volume

197

Issue

10

Start / End Page

4163 / 4176

Location

United States

Related Subject Headings

  • Tetanus Toxoid
  • Receptors, Antigen, T-Cell
  • Lymphocyte Activation
  • Immunology
  • Immunologic Memory
  • Humans
  • Cells, Cultured
  • Cell Proliferation
  • Cell Line
  • Cell Culture Techniques
 

Citation

APA
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Su, K.-Y., Watanabe, A., Yeh, C.-H., Kelsoe, G., & Kuraoka, M. (2016). Efficient Culture of Human Naive and Memory B Cells for Use as APCs. J Immunol, 197(10), 4163–4176. https://doi.org/10.4049/jimmunol.1502193
Su, Kuei-Ying, Akiko Watanabe, Chen-Hao Yeh, Garnett Kelsoe, and Masayuki Kuraoka. “Efficient Culture of Human Naive and Memory B Cells for Use as APCs.J Immunol 197, no. 10 (November 15, 2016): 4163–76. https://doi.org/10.4049/jimmunol.1502193.
Su K-Y, Watanabe A, Yeh C-H, Kelsoe G, Kuraoka M. Efficient Culture of Human Naive and Memory B Cells for Use as APCs. J Immunol. 2016 Nov 15;197(10):4163–76.
Su, Kuei-Ying, et al. “Efficient Culture of Human Naive and Memory B Cells for Use as APCs.J Immunol, vol. 197, no. 10, Nov. 2016, pp. 4163–76. Pubmed, doi:10.4049/jimmunol.1502193.
Su K-Y, Watanabe A, Yeh C-H, Kelsoe G, Kuraoka M. Efficient Culture of Human Naive and Memory B Cells for Use as APCs. J Immunol. 2016 Nov 15;197(10):4163–4176.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

November 15, 2016

Volume

197

Issue

10

Start / End Page

4163 / 4176

Location

United States

Related Subject Headings

  • Tetanus Toxoid
  • Receptors, Antigen, T-Cell
  • Lymphocyte Activation
  • Immunology
  • Immunologic Memory
  • Humans
  • Cells, Cultured
  • Cell Proliferation
  • Cell Line
  • Cell Culture Techniques