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Genome-wide identification of regulatory elements in Sertoli cells.

Publication ,  Journal Article
Maatouk, DM; Natarajan, A; Shibata, Y; Song, L; Crawford, GE; Ohler, U; Capel, B
Published in: Development
February 15, 2017

A current goal of molecular biology is to identify transcriptional networks that regulate cell differentiation. However, identifying functional gene regulatory elements has been challenging in the context of developing tissues where material is limited and cell types are mixed. To identify regulatory sites during sex determination, we subjected Sertoli cells from mouse fetal testes to DNaseI-seq and ChIP-seq for H3K27ac. DNaseI-seq identified putative regulatory sites around genes enriched in Sertoli and pregranulosa cells; however, active enhancers marked by H3K27ac were enriched proximal to only Sertoli-enriched genes. Sequence analysis identified putative binding sites of known and novel transcription factors likely controlling Sertoli cell differentiation. As a validation of this approach, we identified a novel Sertoli cell enhancer upstream of Wt1, and used it to drive expression of a transgenic reporter in Sertoli cells. This work furthers our understanding of the complex genetic network that underlies sex determination and identifies regions that potentially harbor non-coding mutations underlying disorders of sexual development.

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Published In

Development

DOI

EISSN

1477-9129

Publication Date

February 15, 2017

Volume

144

Issue

4

Start / End Page

720 / 730

Location

England

Related Subject Headings

  • Transgenes
  • Testis
  • Sex Determination Processes
  • Sertoli Cells
  • Regulatory Elements, Transcriptional
  • Promoter Regions, Genetic
  • Mutation
  • Mice
  • Male
  • Homozygote
 

Citation

APA
Chicago
ICMJE
MLA
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Maatouk, D. M., Natarajan, A., Shibata, Y., Song, L., Crawford, G. E., Ohler, U., & Capel, B. (2017). Genome-wide identification of regulatory elements in Sertoli cells. Development, 144(4), 720–730. https://doi.org/10.1242/dev.142554
Maatouk, Danielle M., Anirudh Natarajan, Yoichiro Shibata, Lingyun Song, Gregory E. Crawford, Uwe Ohler, and Blanche Capel. “Genome-wide identification of regulatory elements in Sertoli cells.Development 144, no. 4 (February 15, 2017): 720–30. https://doi.org/10.1242/dev.142554.
Maatouk DM, Natarajan A, Shibata Y, Song L, Crawford GE, Ohler U, et al. Genome-wide identification of regulatory elements in Sertoli cells. Development. 2017 Feb 15;144(4):720–30.
Maatouk, Danielle M., et al. “Genome-wide identification of regulatory elements in Sertoli cells.Development, vol. 144, no. 4, Feb. 2017, pp. 720–30. Pubmed, doi:10.1242/dev.142554.
Maatouk DM, Natarajan A, Shibata Y, Song L, Crawford GE, Ohler U, Capel B. Genome-wide identification of regulatory elements in Sertoli cells. Development. 2017 Feb 15;144(4):720–730.
Journal cover image

Published In

Development

DOI

EISSN

1477-9129

Publication Date

February 15, 2017

Volume

144

Issue

4

Start / End Page

720 / 730

Location

England

Related Subject Headings

  • Transgenes
  • Testis
  • Sex Determination Processes
  • Sertoli Cells
  • Regulatory Elements, Transcriptional
  • Promoter Regions, Genetic
  • Mutation
  • Mice
  • Male
  • Homozygote