Skip to main content

Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques.

Publication ,  Journal Article
Chang, WLW; Gonzalez, DF; Kieu, HT; Castillo, LD; Messaoudi, I; Shen, X; Tomaras, GD; Shacklett, BL; Barry, PA; Sparger, EE
Published in: PLoS One
2017

Aging and certain viral infections can negatively impact humoral responses in humans. To further develop the nonhuman primate (NHP) model for investigating B cell dynamics in human aging and infectious disease, a flow cytometric panel was developed to characterize circulating rhesus B cell subsets. Significant differences between human and macaque B cells included the proportions of cells within IgD+ and switched memory populations and a prominent CD21-CD27+ unswitched memory population detected only in macaques. We then utilized the expanded panel to analyze B cell alterations associated with aging and acute simian immunodeficiency virus (SIV) infection in the NHP model. In the aging study, distinct patterns of B cell subset frequencies were observed for macaques aged one to five years compared to those between ages 5 and 30 years. In the SIV infection study, B cell frequencies and absolute number were dramatically reduced following acute infection, but recovered within four weeks of infection. Thereafter, the frequencies of activated memory B cells progressively increased; these were significantly correlated with the magnitude of SIV-specific IgG responses, and coincided with impaired maturation of anti-SIV antibody avidity, as previously reported for HIV-1 infection. These observations further validate the NHP model for investigation of mechanisms responsible for B cells alterations associated with immunosenescence and infectious disease.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2017

Volume

12

Issue

1

Start / End Page

e0170154

Location

United States

Related Subject Headings

  • Viral Load
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Male
  • Macaca mulatta
  • Immunologic Memory
  • General Science & Technology
  • Female
  • B-Lymphocyte Subsets
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chang, W. L. W., Gonzalez, D. F., Kieu, H. T., Castillo, L. D., Messaoudi, I., Shen, X., … Sparger, E. E. (2017). Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques. PLoS One, 12(1), e0170154. https://doi.org/10.1371/journal.pone.0170154
Chang, WL William, Denise F. Gonzalez, Hung T. Kieu, Luis D. Castillo, Ilhem Messaoudi, Xiaoying Shen, Georgia D. Tomaras, Barbara L. Shacklett, Peter A. Barry, and Ellen E. Sparger. “Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques.PLoS One 12, no. 1 (2017): e0170154. https://doi.org/10.1371/journal.pone.0170154.
Chang WLW, Gonzalez DF, Kieu HT, Castillo LD, Messaoudi I, Shen X, et al. Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques. PLoS One. 2017;12(1):e0170154.
Chang, WL William, et al. “Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques.PLoS One, vol. 12, no. 1, 2017, p. e0170154. Pubmed, doi:10.1371/journal.pone.0170154.
Chang WLW, Gonzalez DF, Kieu HT, Castillo LD, Messaoudi I, Shen X, Tomaras GD, Shacklett BL, Barry PA, Sparger EE. Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques. PLoS One. 2017;12(1):e0170154.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2017

Volume

12

Issue

1

Start / End Page

e0170154

Location

United States

Related Subject Headings

  • Viral Load
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Male
  • Macaca mulatta
  • Immunologic Memory
  • General Science & Technology
  • Female
  • B-Lymphocyte Subsets