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APOL1 risk alleles among individuals with CKD in Northern Tanzania: A pilot study.

Publication ,  Journal Article
Stanifer, JW; Karia, F; Maro, V; Kilonzo, K; Qin, X; Patel, UD; Hauser, ER
Published in: PLoS One
2017

INTRODUCTION: In sub-Saharan Africa, approximately 100 million people have CKD, yet genetic risk factors are not well-understood. Despite the potential importance of understanding APOL1 risk allele status among individuals with CKD, little genetic research has been conducted. Therefore, we conducted a pilot study evaluating the feasibility of and willingness to participate in genetic research on kidney disease, and we estimated APOL1 risk allele frequencies among individuals with CKD. METHODS: In 2014, we conducted a community-based field study evaluating CKD epidemiology in northern Tanzania. We assessed for CKD using urine albumin and serum creatinine to estimate GFR. We invited participants with CKD to enroll in an additional genetic study. We obtained dried-blood spots on filter cards, from which we extracted DNA using sterile punch biopsies. We genotyped for two single nucleotide polymorphisms (SNPs) defining the APOL1 G1 risk allele and an insertion/deletion polymorphism defining the G2 risk allele. Genotyping was performed in duplicate. RESULTS: We enrolled 481 participant, 57 (12%) of whom had CKD. Among these, enrollment for genotyping was high (n = 48; 84%). We extracted a median of 19.4 ng of DNA from each dried-blood spot sample, and we genotyped the two APOL1 G1 SNPs and the APOL1 G2 polymorphism. Genotyping quality was high, with all duplicated samples showing perfect concordance. The frequency of APOL1 risk variants ranged from 7.0% to 11.0%, which was similar to previously-reported frequencies from the general population of northern Tanzania (p>0.2). DISCUSSION: In individuals with CKD from northern Tanzania, we demonstrated feasibility of genotyping APOL1 risk alleles. We successfully genotyped three risk variants from DNA extracted from filter cards, and we demonstrated a high enrollment for participation. In this population, more extensive genetic studies of kidney disease may be well-received and will be feasible.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2017

Volume

12

Issue

7

Start / End Page

e0181811

Location

United States

Related Subject Headings

  • Young Adult
  • Tanzania
  • Risk Factors
  • Renal Insufficiency, Chronic
  • Polymorphism, Single Nucleotide
  • Pilot Projects
  • Middle Aged
  • Male
  • Lipoproteins, HDL
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Stanifer, J. W., Karia, F., Maro, V., Kilonzo, K., Qin, X., Patel, U. D., & Hauser, E. R. (2017). APOL1 risk alleles among individuals with CKD in Northern Tanzania: A pilot study. PLoS One, 12(7), e0181811. https://doi.org/10.1371/journal.pone.0181811
Stanifer, John W., Francis Karia, Venance Maro, Kajiru Kilonzo, Xuejun Qin, Uptal D. Patel, and Elizabeth R. Hauser. “APOL1 risk alleles among individuals with CKD in Northern Tanzania: A pilot study.PLoS One 12, no. 7 (2017): e0181811. https://doi.org/10.1371/journal.pone.0181811.
Stanifer JW, Karia F, Maro V, Kilonzo K, Qin X, Patel UD, et al. APOL1 risk alleles among individuals with CKD in Northern Tanzania: A pilot study. PLoS One. 2017;12(7):e0181811.
Stanifer, John W., et al. “APOL1 risk alleles among individuals with CKD in Northern Tanzania: A pilot study.PLoS One, vol. 12, no. 7, 2017, p. e0181811. Pubmed, doi:10.1371/journal.pone.0181811.
Stanifer JW, Karia F, Maro V, Kilonzo K, Qin X, Patel UD, Hauser ER. APOL1 risk alleles among individuals with CKD in Northern Tanzania: A pilot study. PLoS One. 2017;12(7):e0181811.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2017

Volume

12

Issue

7

Start / End Page

e0181811

Location

United States

Related Subject Headings

  • Young Adult
  • Tanzania
  • Risk Factors
  • Renal Insufficiency, Chronic
  • Polymorphism, Single Nucleotide
  • Pilot Projects
  • Middle Aged
  • Male
  • Lipoproteins, HDL
  • Humans