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Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration.

Publication ,  Journal Article
Banks, CJ; Rodriguez, NW; Gashler, KR; Pandya, RR; Mortenson, JB; Whited, MD; Soderblom, EJ; Thompson, JW; Moseley, MA; Reddi, AR; Tessem, JS ...
Published in: Mol Cell Biol
October 15, 2017

In this study, we employed proteomics to identify mechanisms of posttranslational regulation on cell survival signaling proteins. We focused on Cu-Zn superoxide dismutase (SOD1), which protects cells from oxidative stress. We found that acylation of K122 on SOD1, while not impacting SOD1 catalytic activity, suppressed the ability of SOD1 to inhibit mitochondrial metabolism at respiratory complex I. We found that deacylase depletion increased K122 acylation on SOD1, which blocked the suppression of respiration in a K122-dependent manner. In addition, we found that acyl-mimicking mutations at K122 decreased SOD1 accumulation in mitochondria, initially hinting that SOD1 may inhibit respiration directly within the intermembrane space (IMS). However, surprisingly, we found that forcing the K122 acyl mutants into the mitochondria with an IMS-targeting tag did not recover their ability to suppress respiration. Moreover, we found that suppressing or boosting respiration levels toggled SOD1 in or out of the mitochondria, respectively. These findings place SOD1-mediated inhibition of respiration upstream of its mitochondrial localization. Lastly, deletion-rescue experiments show that a respiration-defective mutant of SOD1 is also impaired in its ability to rescue cells from toxicity caused by SOD1 deletion. Together, these data suggest a previously unknown interplay between SOD1 acylation, metabolic regulation, and SOD1-mediated cell survival.

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Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

October 15, 2017

Volume

37

Issue

20

Location

United States

Related Subject Headings

  • Superoxide Dismutase-1
  • Superoxide Dismutase
  • Oxidative Stress
  • Mutation
  • Mitochondrial Membranes
  • Mitochondria
  • Mice
  • Humans
  • Developmental Biology
  • Animals
 

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Banks, C. J., Rodriguez, N. W., Gashler, K. R., Pandya, R. R., Mortenson, J. B., Whited, M. D., … Andersen, J. L. (2017). Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration. Mol Cell Biol, 37(20). https://doi.org/10.1128/MCB.00354-17
Banks, Courtney J., Nathan W. Rodriguez, Kyle R. Gashler, Rushika R. Pandya, Jeffrey B. Mortenson, Matthew D. Whited, Erik J. Soderblom, et al. “Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration.Mol Cell Biol 37, no. 20 (October 15, 2017). https://doi.org/10.1128/MCB.00354-17.
Banks CJ, Rodriguez NW, Gashler KR, Pandya RR, Mortenson JB, Whited MD, et al. Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration. Mol Cell Biol. 2017 Oct 15;37(20).
Banks, Courtney J., et al. “Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration.Mol Cell Biol, vol. 37, no. 20, Oct. 2017. Pubmed, doi:10.1128/MCB.00354-17.
Banks CJ, Rodriguez NW, Gashler KR, Pandya RR, Mortenson JB, Whited MD, Soderblom EJ, Thompson JW, Moseley MA, Reddi AR, Tessem JS, Torres MP, Bikman BT, Andersen JL. Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration. Mol Cell Biol. 2017 Oct 15;37(20).

Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

October 15, 2017

Volume

37

Issue

20

Location

United States

Related Subject Headings

  • Superoxide Dismutase-1
  • Superoxide Dismutase
  • Oxidative Stress
  • Mutation
  • Mitochondrial Membranes
  • Mitochondria
  • Mice
  • Humans
  • Developmental Biology
  • Animals