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Proteomics and immunohistochemistry identify the expression of α-cardiac myosin heavy chain in the jaw-closing muscles of sooty mangabeys (order Primates).

Publication ,  Journal Article
Wall, CE; Holmes, M; Soderblom, EJ; Taylor, AB
Published in: Arch Oral Biol
July 2018

OBJECTIVE: The jaw-closing muscles of humans and nonprimate mammals express alpha-cardiac fibers but MyHC α-cardiac has not been identified in the jaw adductors of nonhuman primates. We determined whether MyHC α-cardiac is expressed in the superficial masseter and temporalis muscles of the sooty mangabey (Cercocebus atys), an African Old World monkey that specializes on hard seeds. DESIGN: LC-MS/MS based proteomics was used to identify the presence of MyHC Iα. Immunohistochemistry was used to analyze the composition and distribution of fiber types in the superficial masseter and temporalis muscles of eight C. atys. Serial sections were stained against MyHC α-cardiac (MYH6), as well as MyHC-1 (NOQ7.5.4D), MyHC-2 (MY-32), and MyHC-M (2F4). RESULTS: Proteomics analysis identified the presence of Myosin-6 (MyHC α-cardiac) in both heart atrium and superficial masseter. MyHC α-cardiac was expressed in abundance in the superficial masseter and temporalis muscles of all eight individuals and hybrid fibers were common. CONCLUSIONS: The identification of MyHC α-cardiac in the jaw adductors of sooty mangabeys is a novel finding for nonhuman primates. The abundance of MyHC α-cardiac indicates a fatigue-resistant fiber population characterized by intermediate speed of contraction between pure MyHC-1 and MyHC-2 isoforms. We suggest that α-cardiac fibers may be advantageous to sooty mangabeys, whose feeding behavior includes frequent crushing of relatively large, hard seeds during the power stroke of ingestion. Additional studies comparing jaw-adductor fiber phenotype of hard-object feeding primates and other mammals are needed to explore this relationship further.

Duke Scholars

Published In

Arch Oral Biol

DOI

EISSN

1879-1506

Publication Date

July 2018

Volume

91

Start / End Page

103 / 108

Location

England

Related Subject Headings

  • Ventricular Myosins
  • Temporal Muscle
  • Proteomics
  • Protein Isoforms
  • Primates
  • Myosin Heavy Chains
  • Masseter Muscle
  • Male
  • Immunohistochemistry
  • Humans
 

Citation

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ICMJE
MLA
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Wall, C. E., Holmes, M., Soderblom, E. J., & Taylor, A. B. (2018). Proteomics and immunohistochemistry identify the expression of α-cardiac myosin heavy chain in the jaw-closing muscles of sooty mangabeys (order Primates). Arch Oral Biol, 91, 103–108. https://doi.org/10.1016/j.archoralbio.2018.01.019
Wall, Christine E., Megan Holmes, Erik J. Soderblom, and Andrea B. Taylor. “Proteomics and immunohistochemistry identify the expression of α-cardiac myosin heavy chain in the jaw-closing muscles of sooty mangabeys (order Primates).Arch Oral Biol 91 (July 2018): 103–8. https://doi.org/10.1016/j.archoralbio.2018.01.019.
Wall, Christine E., et al. “Proteomics and immunohistochemistry identify the expression of α-cardiac myosin heavy chain in the jaw-closing muscles of sooty mangabeys (order Primates).Arch Oral Biol, vol. 91, July 2018, pp. 103–08. Pubmed, doi:10.1016/j.archoralbio.2018.01.019.
Journal cover image

Published In

Arch Oral Biol

DOI

EISSN

1879-1506

Publication Date

July 2018

Volume

91

Start / End Page

103 / 108

Location

England

Related Subject Headings

  • Ventricular Myosins
  • Temporal Muscle
  • Proteomics
  • Protein Isoforms
  • Primates
  • Myosin Heavy Chains
  • Masseter Muscle
  • Male
  • Immunohistochemistry
  • Humans