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A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration.

Publication ,  Journal Article
Xiong, C; Zhang, Z; Baht, GS; Terrando, N
Published in: J Vis Exp
February 27, 2018

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and significantly diminish the outcome. Indeed, after routine orthopedic surgery to repair a fracture, as many as 50% of elderly patients suffer from neurologic complications like delirium. Also, the capacity to heal and regenerate tissue after surgery decreases with age, and can impact the quality of fracture repair and even osseous integration of implants. Thus, a better understanding of mechanisms that drive these age-dependent changes could provide strategic targets to minimize risk for such complications and optimize outcomes. Here, we introduce a clinically relevant mouse model of tibial fracture. The postoperative changes in these mice mimic some of the cognitive impairments commonly observed after routine orthopedic surgery in humans. Briefly, an incision is performed in the right hind limb under strictly aseptic conditions. Muscles are disassociated, and a 0.38-mm stainless steel pin is inserted into the upper crest of the tibia, inside the intramedullary canal. Osteotomy is then performed, and the wound is stapled. We have used this model to investigate the effects of surgical trauma on postoperative neuroinflammation and behavioral changes. By applying this fracture model in combination with parabiosis, a surgical model in which 2 mice are anastomosed, we have studied cells and secreted factors that systemically rejuvenate organ function and tissue regeneration after injury. By following our step-by-step protocol, these models can be reproduced with high fidelity, and can be adapted to interrogate many biologic pathways that are altered by surgical trauma.

Duke Scholars

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

February 27, 2018

Issue

132

Location

United States

Related Subject Headings

  • Postoperative Complications
  • Orthopedics
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Humans
  • Guided Tissue Regeneration
  • Female
  • Disease Models, Animal
  • Cognitive Dysfunction
 

Citation

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MLA
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Xiong, C., Zhang, Z., Baht, G. S., & Terrando, N. (2018). A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration. J Vis Exp, (132). https://doi.org/10.3791/56701
Xiong, Chao, Zhiquan Zhang, Gurpreet S. Baht, and Niccolo Terrando. “A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration.J Vis Exp, no. 132 (February 27, 2018). https://doi.org/10.3791/56701.
Xiong, Chao, et al. “A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration.J Vis Exp, no. 132, Feb. 2018. Pubmed, doi:10.3791/56701.

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

February 27, 2018

Issue

132

Location

United States

Related Subject Headings

  • Postoperative Complications
  • Orthopedics
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Humans
  • Guided Tissue Regeneration
  • Female
  • Disease Models, Animal
  • Cognitive Dysfunction