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Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques.

Publication ,  Journal Article
Ram, DR; Manickam, C; Hueber, B; Itell, HL; Permar, SR; Varner, V; Reeves, RK
Published in: PLoS Pathog
May 2018

Natural killer (NK) cells classically typify the nonspecific effector arm of the innate immune system, but have recently been shown to possess memory-like properties against multiple viral infections, most notably CMV. Expression of the activating receptor NKG2C is elevated on human NK cells in response to infection with CMV as well as HIV, and may delineate cells with memory and memory-like functions. A better understanding of how NKG2C+ NK cells specifically respond to these pathogens could be significantly advanced using nonhuman primate (NHP) models but, to date, it has not been possible to distinguish NKG2C from its inhibitory counterpart, NKG2A, in NHP because of unfaithful antibody cross-reactivity. Using novel RNA-based flow cytometry, we identify for the first time true memory NKG2C+ NK cells in NHP by gene expression (KLRC2), and show that these cells have elevated frequencies and diversify their functional repertoire specifically in response to rhCMV and SIV infections.

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Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

May 2018

Volume

14

Issue

5

Start / End Page

e1007104

Location

United States

Related Subject Headings

  • Virology
  • NK Cell Lectin-Like Receptor Subfamily C
  • Macaca mulatta
  • Killer Cells, Natural
  • Humans
  • Cytomegalovirus Infections
  • Cytomegalovirus
  • CD57 Antigens
  • Animals
  • 3207 Medical microbiology
 

Citation

APA
Chicago
ICMJE
MLA
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Ram, D. R., Manickam, C., Hueber, B., Itell, H. L., Permar, S. R., Varner, V., & Reeves, R. K. (2018). Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques. PLoS Pathog, 14(5), e1007104. https://doi.org/10.1371/journal.ppat.1007104
Ram, Daniel R., Cordelia Manickam, Brady Hueber, Hannah L. Itell, Sallie R. Permar, Valerie Varner, and R Keith Reeves. “Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques.PLoS Pathog 14, no. 5 (May 2018): e1007104. https://doi.org/10.1371/journal.ppat.1007104.
Ram DR, Manickam C, Hueber B, Itell HL, Permar SR, Varner V, et al. Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques. PLoS Pathog. 2018 May;14(5):e1007104.
Ram, Daniel R., et al. “Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques.PLoS Pathog, vol. 14, no. 5, May 2018, p. e1007104. Pubmed, doi:10.1371/journal.ppat.1007104.
Ram DR, Manickam C, Hueber B, Itell HL, Permar SR, Varner V, Reeves RK. Tracking KLRC2 (NKG2C)+ memory-like NK cells in SIV+ and rhCMV+ rhesus macaques. PLoS Pathog. 2018 May;14(5):e1007104.

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

May 2018

Volume

14

Issue

5

Start / End Page

e1007104

Location

United States

Related Subject Headings

  • Virology
  • NK Cell Lectin-Like Receptor Subfamily C
  • Macaca mulatta
  • Killer Cells, Natural
  • Humans
  • Cytomegalovirus Infections
  • Cytomegalovirus
  • CD57 Antigens
  • Animals
  • 3207 Medical microbiology