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Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays.

Publication ,  Journal Article
Fontes, CM; Achar, RK; Joh, DY; Ozer, I; Bhattacharjee, S; Hucknall, A; Chilkoti, A
Published in: Langmuir : the ACS journal of surfaces and colloids
February 2019

Motivated by the lack of adventitious protein adsorption on zwitterionic polymer brushes that promise low noise and hence high analytical sensitivity for surface-based immunoassays, we explored their use as a substrate for immunoassay fabrication by the inkjet printing of antibodies. We observed that a poly(sulfobetaine)methacrylate brush on glass is far too hydrophilic to enable the noncovalent immobilization of antibodies by inkjet printing. To circumvent this limitation, we developed a series of hybrid zwitterionic-cationic surface coatings with tunable surface wettability that are suitable for the inkjet printing of antibodies but also have low protein adsorption. We show that in a microarray format in which both the capture and detection antibodies are discretely printed as spots on these hybrid brushes, a point-of-care sandwich immunoassay can be carried out with an analytical sensitivity and dynamic range that is similar to or better than those of the same assay fabricated on a PEG-like brush. We also show that the hybrid polymer brushes do not bind anti-PEG antibodies that are ubiquitous in human blood, which can be a problem with immunoassays fabricated on PEG-like coatings.

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Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

February 2019

Volume

35

Issue

5

Start / End Page

1379 / 1390

Related Subject Headings

  • Wettability
  • Rabbits
  • Printing
  • Point-of-Care Testing
  • Methacrylates
  • Interleukin-6
  • Immunoassay
  • Humans
  • Chemical Physics
  • Cattle
 

Citation

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Fontes, C. M., Achar, R. K., Joh, D. Y., Ozer, I., Bhattacharjee, S., Hucknall, A., & Chilkoti, A. (2019). Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays. Langmuir : The ACS Journal of Surfaces and Colloids, 35(5), 1379–1390. https://doi.org/10.1021/acs.langmuir.8b01597
Fontes, Cassio M., Rohan K. Achar, Daniel Y. Joh, Imran Ozer, Somnath Bhattacharjee, Angus Hucknall, and Ashutosh Chilkoti. “Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays.Langmuir : The ACS Journal of Surfaces and Colloids 35, no. 5 (February 2019): 1379–90. https://doi.org/10.1021/acs.langmuir.8b01597.
Fontes CM, Achar RK, Joh DY, Ozer I, Bhattacharjee S, Hucknall A, et al. Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays. Langmuir : the ACS journal of surfaces and colloids. 2019 Feb;35(5):1379–90.
Fontes, Cassio M., et al. “Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays.Langmuir : The ACS Journal of Surfaces and Colloids, vol. 35, no. 5, Feb. 2019, pp. 1379–90. Epmc, doi:10.1021/acs.langmuir.8b01597.
Fontes CM, Achar RK, Joh DY, Ozer I, Bhattacharjee S, Hucknall A, Chilkoti A. Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays. Langmuir : the ACS journal of surfaces and colloids. 2019 Feb;35(5):1379–1390.
Journal cover image

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

February 2019

Volume

35

Issue

5

Start / End Page

1379 / 1390

Related Subject Headings

  • Wettability
  • Rabbits
  • Printing
  • Point-of-Care Testing
  • Methacrylates
  • Interleukin-6
  • Immunoassay
  • Humans
  • Chemical Physics
  • Cattle