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Characterization of the Transmitted Virus in an Ongoing HIV-1 Epidemic Driven by Injecting Drug Use.

Publication ,  Journal Article
Dukhovlinova, E; Masharsky, A; Vasileva, A; Porrello, A; Zhou, S; Toussova, O; Verevochkin, S; Akulova, E; Frishman, D; Montefiori, D ...
Published in: AIDS Res Hum Retroviruses
October 2018

Understanding features of the HIV-1 transmission process has the potential to inform biological interventions for prevention. We have examined the transmitted virus in a cohort of people who inject drugs and who are at risk of HIV-1 infection through blood contamination when injecting in a group. This study focused on seven newly infected participants in St. Petersburg, Russia, who were in acute or early infection. We used end-point dilution polymerase chain reaction to amplify single viral genomes to assess the complexity of the transmitted virus. We also used deep sequencing to further assess the complexity of the virus. We interpret the results as indicating that a single viral variant was transmitted in each case, consistent with a model where the exposure to virus during transmission was limited. We also looked at phenotypic properties of the viral Env protein in isolates from acute and chronic infection. Although differences were noted, there was no consistent pattern that distinguished the transmitted variants. Similarly, despite the reduced genetic heterogeneity of the more recent subtype A HIV-1 epidemic in St. Petersburg, we did not see reduced variance in the neutralization properties compared to isolates from the more mature subtype C HIV-1 epidemic. Finally, in looking at members of injecting groups related to the acute HIV-1 infection/early subjects, we found examples of sequence linkage consistent with ongoing and rapid spread of HIV-1 in these groups. These studies emphasize the dynamic nature of this epidemic and reinforce the idea that improved prevention methods are needed.

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Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

October 2018

Volume

34

Issue

10

Start / End Page

867 / 878

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Substance Abuse, Intravenous
  • Sequence Analysis, DNA
  • Russia
  • Polymerase Chain Reaction
  • Phylogeny
  • Neutralization Tests
  • Molecular Epidemiology
  • Humans
 

Citation

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Dukhovlinova, E., Masharsky, A., Vasileva, A., Porrello, A., Zhou, S., Toussova, O., … Swanstrom, R. (2018). Characterization of the Transmitted Virus in an Ongoing HIV-1 Epidemic Driven by Injecting Drug Use. AIDS Res Hum Retroviruses, 34(10), 867–878. https://doi.org/10.1089/AID.2017.0313
Dukhovlinova, Elena, Alexey Masharsky, Aleksandra Vasileva, Alessandro Porrello, Shuntai Zhou, Olga Toussova, Sergei Verevochkin, et al. “Characterization of the Transmitted Virus in an Ongoing HIV-1 Epidemic Driven by Injecting Drug Use.AIDS Res Hum Retroviruses 34, no. 10 (October 2018): 867–78. https://doi.org/10.1089/AID.2017.0313.
Dukhovlinova E, Masharsky A, Vasileva A, Porrello A, Zhou S, Toussova O, et al. Characterization of the Transmitted Virus in an Ongoing HIV-1 Epidemic Driven by Injecting Drug Use. AIDS Res Hum Retroviruses. 2018 Oct;34(10):867–78.
Dukhovlinova, Elena, et al. “Characterization of the Transmitted Virus in an Ongoing HIV-1 Epidemic Driven by Injecting Drug Use.AIDS Res Hum Retroviruses, vol. 34, no. 10, Oct. 2018, pp. 867–78. Pubmed, doi:10.1089/AID.2017.0313.
Dukhovlinova E, Masharsky A, Vasileva A, Porrello A, Zhou S, Toussova O, Verevochkin S, Akulova E, Frishman D, Montefiori D, Labranche C, Hoffman I, Miller W, Cohen MS, Kozlov AP, Swanstrom R. Characterization of the Transmitted Virus in an Ongoing HIV-1 Epidemic Driven by Injecting Drug Use. AIDS Res Hum Retroviruses. 2018 Oct;34(10):867–878.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

October 2018

Volume

34

Issue

10

Start / End Page

867 / 878

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Substance Abuse, Intravenous
  • Sequence Analysis, DNA
  • Russia
  • Polymerase Chain Reaction
  • Phylogeny
  • Neutralization Tests
  • Molecular Epidemiology
  • Humans