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In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model.

Publication ,  Journal Article
Li, G; Lee, C; Agrahari, V; Wang, K; Navarro, I; Sherwood, JM; Crews, K; Farsiu, S; Gonzalez, P; Lin, C-W; Mitra, AK; Ethier, CR; Stamer, WD
Published in: Proc Natl Acad Sci U S A
January 29, 2019

Ocular corticosteroids are commonly used clinically. Unfortunately, their administration frequently leads to ocular hypertension, i.e., elevated intraocular pressure (IOP), which, in turn, can progress to a form of glaucoma known as steroid-induced glaucoma. The pathophysiology of this condition is poorly understood yet shares similarities with the most common form of glaucoma. Using nanotechnology, we created a mouse model of corticosteroid-induced ocular hypertension. This model functionally and morphologically resembles human ocular hypertension, having titratable, robust, and sustained IOPs caused by increased resistance to aqueous humor outflow. Using this model, we then interrogated the biomechanical properties of the trabecular meshwork (TM), including the inner wall of Schlemm's canal (SC), tissues known to strongly influence IOP and to be altered in other forms of glaucoma. Specifically, using spectral domain optical coherence tomography, we observed that SC in corticosteroid-treated mice was more resistant to collapse at elevated IOPs, reflecting increased TM stiffness determined by inverse finite element modeling. Our noninvasive approach to monitoring TM stiffness in vivo is applicable to other forms of glaucoma and has significant potential to monitor TM function and thus positively affect the clinical care of glaucoma, the leading cause of irreversible blindness worldwide.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

January 29, 2019

Volume

116

Issue

5

Start / End Page

1714 / 1722

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Tomography, Optical Coherence
  • Mice, Inbred C57BL
  • Mice
  • Intraocular Pressure
  • Hypertension
  • Glaucoma
  • Disease Models, Animal
  • Blindness
  • Aqueous Humor
 

Citation

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Li, G., Lee, C., Agrahari, V., Wang, K., Navarro, I., Sherwood, J. M., … Stamer, W. D. (2019). In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model. Proc Natl Acad Sci U S A, 116(5), 1714–1722. https://doi.org/10.1073/pnas.1814889116
Li, Guorong, Chanyoung Lee, Vibhuti Agrahari, Ke Wang, Iris Navarro, Joseph M. Sherwood, Karen Crews, et al. “In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model.Proc Natl Acad Sci U S A 116, no. 5 (January 29, 2019): 1714–22. https://doi.org/10.1073/pnas.1814889116.
Li G, Lee C, Agrahari V, Wang K, Navarro I, Sherwood JM, et al. In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model. Proc Natl Acad Sci U S A. 2019 Jan 29;116(5):1714–22.
Li, Guorong, et al. “In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model.Proc Natl Acad Sci U S A, vol. 116, no. 5, Jan. 2019, pp. 1714–22. Pubmed, doi:10.1073/pnas.1814889116.
Li G, Lee C, Agrahari V, Wang K, Navarro I, Sherwood JM, Crews K, Farsiu S, Gonzalez P, Lin C-W, Mitra AK, Ethier CR, Stamer WD. In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model. Proc Natl Acad Sci U S A. 2019 Jan 29;116(5):1714–1722.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

January 29, 2019

Volume

116

Issue

5

Start / End Page

1714 / 1722

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Tomography, Optical Coherence
  • Mice, Inbred C57BL
  • Mice
  • Intraocular Pressure
  • Hypertension
  • Glaucoma
  • Disease Models, Animal
  • Blindness
  • Aqueous Humor