Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Thyroid receptor antagonism as a contributory mechanism for adipogenesis induced by environmental mixtures in 3T3-L1 cells.

Publication ,  Journal Article
Kassotis, CD; Kollitz, EM; Hoffman, K; Sosa, JA; Stapleton, HM
Published in: The Science of the total environment
May 2019

We previously demonstrated that indoor house dust extracts could induce adipogenesis in pre-adipocytes, suggesting a potential role for indoor contaminant mixtures in metabolic health. Herein, we investigated the potential role of thyroid receptor beta (TRβ) antagonism in adipogenic effects (dust-induced triglyceride accumulation and pre-adipocyte proliferation) following exposure to environmental mixtures (indoor house dust extracts). Concentrations of specific flame retardants were measured in extracts, and metabolic health information was collected from residents (n = 137). 90% of dust extracts exhibited significant adipogenic activity, >60% via triglyceride accumulation, and >70% via pre-adipocyte proliferation. Triglyceride accumulation was positively correlated with concentrations of each of twelve flame retardants, despite most being independently inactive; this suggests a putative role for co-exposures or mixtures. We further reported a positive correlation between dust-induced triglyceride accumulation and serum thyroid stimulating hormone concentrations, negative correlations with serum free triiodothyronine and thyroxine concentrations, and a positive and significant association between dust-induced triglyceride accumulation and residents' body mass index (BMI). We hypothesized that inhibition of TR antagonism might counteract these effects, and both addition of a TR agonist and siRNA knock-down of TR resulted in decreased dust-induced triglyceride accumulation in a subset of samples, bolstering this as a contributory mechanism. These results highlight a contributory role of environmental TR antagonism as a putative factor in metabolic health, suggesting that further research should evaluate this mechanism and determine whether in vitro adipogenic activity could have utility as a biomarker for metabolic health in residents.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

The Science of the total environment

DOI

EISSN

1879-1026

ISSN

0048-9697

Publication Date

May 2019

Volume

666

Start / End Page

431 / 444

Related Subject Headings

  • Triglycerides
  • Thyroid Hormone Receptors beta
  • Middle Aged
  • Mice
  • Male
  • Humans
  • Female
  • Environmental Sciences
  • Dust
  • Dose-Response Relationship, Drug
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kassotis, C. D., Kollitz, E. M., Hoffman, K., Sosa, J. A., & Stapleton, H. M. (2019). Thyroid receptor antagonism as a contributory mechanism for adipogenesis induced by environmental mixtures in 3T3-L1 cells. The Science of the Total Environment, 666, 431–444. https://doi.org/10.1016/j.scitotenv.2019.02.273
Kassotis, Christopher D., Erin M. Kollitz, Kate Hoffman, Julie Ann Sosa, and Heather M. Stapleton. “Thyroid receptor antagonism as a contributory mechanism for adipogenesis induced by environmental mixtures in 3T3-L1 cells.The Science of the Total Environment 666 (May 2019): 431–44. https://doi.org/10.1016/j.scitotenv.2019.02.273.
Kassotis CD, Kollitz EM, Hoffman K, Sosa JA, Stapleton HM. Thyroid receptor antagonism as a contributory mechanism for adipogenesis induced by environmental mixtures in 3T3-L1 cells. The Science of the total environment. 2019 May;666:431–44.
Kassotis, Christopher D., et al. “Thyroid receptor antagonism as a contributory mechanism for adipogenesis induced by environmental mixtures in 3T3-L1 cells.The Science of the Total Environment, vol. 666, May 2019, pp. 431–44. Epmc, doi:10.1016/j.scitotenv.2019.02.273.
Kassotis CD, Kollitz EM, Hoffman K, Sosa JA, Stapleton HM. Thyroid receptor antagonism as a contributory mechanism for adipogenesis induced by environmental mixtures in 3T3-L1 cells. The Science of the total environment. 2019 May;666:431–444.
Journal cover image

Published In

The Science of the total environment

DOI

EISSN

1879-1026

ISSN

0048-9697

Publication Date

May 2019

Volume

666

Start / End Page

431 / 444

Related Subject Headings

  • Triglycerides
  • Thyroid Hormone Receptors beta
  • Middle Aged
  • Mice
  • Male
  • Humans
  • Female
  • Environmental Sciences
  • Dust
  • Dose-Response Relationship, Drug