Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells.

Publication ,  Journal Article
Simon, JR; Eghtesadi, SA; Dzuricky, M; You, L; Chilkoti, A
Published in: Molecular cell
July 2019

Liquid granules rich in intrinsically disordered proteins and RNA play key roles in critical cellular functions such as RNA processing and translation. Many details of the mechanism via which this occurs remain to be elucidated. Motivated by the lacuna in the field and by the prospects of developing de novo artificial granules that provide extrinsic control of translation, we report a bottom-up approach to engineer ribonucleoprotein granules composed of a recombinant RNA-binding IDP that exhibits phase behavior in water. We developed a kinetic model to illustrate that these granules inhibit translation through reversible or irreversible sequestration of mRNA. Within monodisperse droplets capable of transcription and translation, we experimentally demonstrate temporal inhibition of translation by using designer IDPs that exhibit tunable phase behavior. This work lays the foundation for developing artificial granules that promise to further our mechanistic understanding of their naturally occurring counterparts.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Molecular cell

DOI

EISSN

1097-4164

ISSN

1097-2765

Publication Date

July 2019

Volume

75

Issue

1

Start / End Page

66 / 75.e5

Related Subject Headings

  • Ribonucleoproteins
  • Recombinant Proteins
  • RNA, Messenger
  • RNA
  • Protein Engineering
  • Protein Biosynthesis
  • Plasmids
  • Phase Transition
  • Peptidomimetics
  • Models, Biological
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Simon, J. R., Eghtesadi, S. A., Dzuricky, M., You, L., & Chilkoti, A. (2019). Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells. Molecular Cell, 75(1), 66-75.e5. https://doi.org/10.1016/j.molcel.2019.05.010
Simon, Joseph R., Seyed Ali Eghtesadi, Michael Dzuricky, Lingchong You, and Ashutosh Chilkoti. “Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells.Molecular Cell 75, no. 1 (July 2019): 66-75.e5. https://doi.org/10.1016/j.molcel.2019.05.010.
Simon JR, Eghtesadi SA, Dzuricky M, You L, Chilkoti A. Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells. Molecular cell. 2019 Jul;75(1):66-75.e5.
Simon, Joseph R., et al. “Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells.Molecular Cell, vol. 75, no. 1, July 2019, pp. 66-75.e5. Epmc, doi:10.1016/j.molcel.2019.05.010.
Simon JR, Eghtesadi SA, Dzuricky M, You L, Chilkoti A. Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells. Molecular cell. 2019 Jul;75(1):66-75.e5.
Journal cover image

Published In

Molecular cell

DOI

EISSN

1097-4164

ISSN

1097-2765

Publication Date

July 2019

Volume

75

Issue

1

Start / End Page

66 / 75.e5

Related Subject Headings

  • Ribonucleoproteins
  • Recombinant Proteins
  • RNA, Messenger
  • RNA
  • Protein Engineering
  • Protein Biosynthesis
  • Plasmids
  • Phase Transition
  • Peptidomimetics
  • Models, Biological