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Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.

Publication ,  Journal Article
Zingl, FG; Kohl, P; Cakar, F; Leitner, DR; Mitterer, F; Bonnington, KE; Rechberger, GN; Kuehn, MJ; Guan, Z; Reidl, J; Schild, S
Published in: Cell Host Microbe
February 12, 2020

Gram-negative bacteria release outer membrane vesicles into the external milieu to deliver effector molecules that alter the host and facilitate virulence. Vesicle formation is driven by phospholipid accumulation in the outer membrane and regulated by the phospholipid transporter VacJ/Yrb. We use the facultative human pathogen Vibrio cholerae to show that VacJ/Yrb is silenced early during mammalian infection, which stimulates vesiculation that expedites bacterial surface exchange and adaptation to the host environment. Hypervesiculating strains rapidly alter their bacterial membrane composition and exhibit enhanced intestinal colonization fitness. This adaptation is exemplified by faster accumulation of glycine-modified lipopolysaccharide (LPS) and depletion of outer membrane porin OmpT, which confers resistance to host-derived antimicrobial peptides and bile, respectively. The competitive advantage of hypervesiculation is lost upon pre-adaptation to bile and antimicrobial peptides, indicating the importance of these adaptive processes. Thus, bacteria use outer membrane vesiculation to exchange cell surface components, thereby increasing survival during mammalian infection.

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Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

February 12, 2020

Volume

27

Issue

2

Start / End Page

225 / 237.e8

Location

United States

Related Subject Headings

  • Vibrio cholerae
  • Transport Vesicles
  • Porins
  • Mice
  • Immunology
  • Host Microbial Interactions
  • Bile
  • Bacterial Proteins
  • Bacterial Outer Membrane Proteins
  • Bacterial Outer Membrane
 

Citation

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Zingl, F. G., Kohl, P., Cakar, F., Leitner, D. R., Mitterer, F., Bonnington, K. E., … Schild, S. (2020). Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae. Cell Host Microbe, 27(2), 225-237.e8. https://doi.org/10.1016/j.chom.2019.12.002
Zingl, Franz G., Paul Kohl, Fatih Cakar, Deborah R. Leitner, Fabian Mitterer, Katherine E. Bonnington, Gerald N. Rechberger, et al. “Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.Cell Host Microbe 27, no. 2 (February 12, 2020): 225-237.e8. https://doi.org/10.1016/j.chom.2019.12.002.
Zingl FG, Kohl P, Cakar F, Leitner DR, Mitterer F, Bonnington KE, et al. Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae. Cell Host Microbe. 2020 Feb 12;27(2):225-237.e8.
Zingl, Franz G., et al. “Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.Cell Host Microbe, vol. 27, no. 2, Feb. 2020, pp. 225-237.e8. Pubmed, doi:10.1016/j.chom.2019.12.002.
Zingl FG, Kohl P, Cakar F, Leitner DR, Mitterer F, Bonnington KE, Rechberger GN, Kuehn MJ, Guan Z, Reidl J, Schild S. Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae. Cell Host Microbe. 2020 Feb 12;27(2):225-237.e8.
Journal cover image

Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

February 12, 2020

Volume

27

Issue

2

Start / End Page

225 / 237.e8

Location

United States

Related Subject Headings

  • Vibrio cholerae
  • Transport Vesicles
  • Porins
  • Mice
  • Immunology
  • Host Microbial Interactions
  • Bile
  • Bacterial Proteins
  • Bacterial Outer Membrane Proteins
  • Bacterial Outer Membrane