Cold sensitivity of the SARS-CoV-2 spike ectodomain.
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, Journal Article
Edwards, RJ; Mansouri, K; Stalls, V; Manne, K; Watts, B; Parks, R; Janowska, K; Gobeil, SMC; Kopp, M; Li, D; Lu, X; Mu, Z; Deyton, M ...
Published in: bioRxiv
October 13, 2020
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible "up" or receptor-inaccessible "down" conformations. Here, we report that the commonly used stabilized S ectodomain construct "2P" is sensitive to cold temperature, and that this cold sensitivity is resolved in a "down" state stabilized spike. Our results will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
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bioRxiv
DOI
EISSN
2692-8205
Publication Date
October 13, 2020
Location
United States
Related Subject Headings
- Developmental Biology
- Biophysics
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 11 Medical and Health Sciences
- 06 Biological Sciences
- 03 Chemical Sciences
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Edwards, R. J., Mansouri, K., Stalls, V., Manne, K., Watts, B., Parks, R., … Acharya, P. (2020). Cold sensitivity of the SARS-CoV-2 spike ectodomain. BioRxiv. https://doi.org/10.1101/2020.07.12.199588
Edwards, Robert J., Katayoun Mansouri, Victoria Stalls, Kartik Manne, Brian Watts, Rob Parks, Katarzyna Janowska, et al. “Cold sensitivity of the SARS-CoV-2 spike ectodomain.” BioRxiv, October 13, 2020. https://doi.org/10.1101/2020.07.12.199588.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, et al. Cold sensitivity of the SARS-CoV-2 spike ectodomain. bioRxiv. 2020 Oct 13;
Edwards, Robert J., et al. “Cold sensitivity of the SARS-CoV-2 spike ectodomain.” BioRxiv, Oct. 2020. Pubmed, doi:10.1101/2020.07.12.199588.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, Janowska K, Gobeil SMC, Kopp M, Li D, Lu X, Mu Z, Deyton M, Oguin TH, Sprenz J, Williams W, Saunders K, Montefiori D, Sempowski GD, Henderson R, Alam M, Haynes BF, Acharya P. Cold sensitivity of the SARS-CoV-2 spike ectodomain. bioRxiv. 2020 Oct 13;
Published In
bioRxiv
DOI
EISSN
2692-8205
Publication Date
October 13, 2020
Location
United States
Related Subject Headings
- Developmental Biology
- Biophysics
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 11 Medical and Health Sciences
- 06 Biological Sciences
- 03 Chemical Sciences