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DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona.

Publication ,  Journal Article
Griffith, DM; Jayaram, DT; Spencer, DM; Pisetsky, DS; Payne, CK
Published in: Biointerphases
October 1, 2020

There has been much recent interest in the protein "corona," the nonspecific adsorption of proteins on the surface of nanoparticles used in biological applications. This research investigates an analogous DNA corona. We find that particles (200 nm and 1 μm) incubated with DNA form a DNA corona, with a higher concentration of DNA adsorbed on the surface of cationic nanoparticles. With protein present, a combined DNA and protein corona is formed although DNA in solution displaces protein from the nanoparticle surface. Displacement of protein from the nanoparticle surface is dependent on the concentration of DNA in solution and was also observed for planar surfaces. Overall, we expect this investigation of the DNA corona to be important for nanomedicine applications, as well as disease states, especially systemic lupus erythematosus, in which biological particles with bound DNA are important mediators of inflammation and thrombosis.

Duke Scholars

Published In

Biointerphases

DOI

EISSN

1559-4106

Publication Date

October 1, 2020

Volume

15

Issue

5

Start / End Page

051006

Location

United States

Related Subject Headings

  • Surface Properties
  • Serum Albumin, Bovine
  • Protein Corona
  • Polystyrenes
  • Nanoparticles
  • DNA
  • Cattle
  • Cations
  • Animals
  • Adsorption
 

Citation

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Griffith, D. M., Jayaram, D. T., Spencer, D. M., Pisetsky, D. S., & Payne, C. K. (2020). DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona. Biointerphases, 15(5), 051006. https://doi.org/10.1116/6.0000439
Griffith, Darbi M., Dhanya T. Jayaram, Diane M. Spencer, David S. Pisetsky, and Christine K. Payne. “DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona.Biointerphases 15, no. 5 (October 1, 2020): 051006. https://doi.org/10.1116/6.0000439.
Griffith DM, Jayaram DT, Spencer DM, Pisetsky DS, Payne CK. DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona. Biointerphases. 2020 Oct 1;15(5):051006.
Griffith, Darbi M., et al. “DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona.Biointerphases, vol. 15, no. 5, Oct. 2020, p. 051006. Pubmed, doi:10.1116/6.0000439.
Griffith DM, Jayaram DT, Spencer DM, Pisetsky DS, Payne CK. DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona. Biointerphases. 2020 Oct 1;15(5):051006.

Published In

Biointerphases

DOI

EISSN

1559-4106

Publication Date

October 1, 2020

Volume

15

Issue

5

Start / End Page

051006

Location

United States

Related Subject Headings

  • Surface Properties
  • Serum Albumin, Bovine
  • Protein Corona
  • Polystyrenes
  • Nanoparticles
  • DNA
  • Cattle
  • Cations
  • Animals
  • Adsorption