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Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells.

Publication ,  Journal Article
Rickard, AG; Zhuang, M; DeRosa, CA; Zhang, X; Dewhirst, MW; Fraser, CL; Palmer, GM
Published in: J Biomed Opt
November 2020

SIGNIFICANCE: Decreasing the oxygen consumption rate (OCR) of tumor cells is a powerful method for ameliorating tumor hypoxia. However, quantifying the change in OCR is challenging in complex experimental systems. AIM: We present a method for quantifying the OCR of two tumor cell lines using oxygen-sensitive dual-emissive boron nanoparticles (BNPs). We hypothesize that our BNP results are equivalent to the standard Seahorse assay. APPROACH: We quantified the spectral emissions of the BNP and accounted for external oxygen diffusion to quantify OCR over 24 h. The BNP-computed OCR of two breast cancer cell lines, E0771 and 4T07, were compared with their respective Seahorse assays. Both cell lines were also irradiated to quantify radiation-induced changes in the OCR. RESULTS: Using a Bland-Altman analysis, our BNPs OCR was equivalent to the standard Seahorse assay. Moreover, in an additional experiment in which we irradiated the cells at their 50% survival fraction, the BNPs were sensitive enough to quantify 24% reduction in OCR after irradiation. CONCLUSIONS: Our results conclude that the BNPs are a viable alternative to the Seahorse assay for quantifying the OCR in cells. The Bland-Altman analysis showed that these two methods result in equivalent OCR measurements. Future studies will extend the OCR measurements to complex systems including 3D cultures and in vivo models, in which OCR measurements cannot currently be made.

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Published In

J Biomed Opt

DOI

EISSN

1560-2281

Publication Date

November 2020

Volume

25

Issue

11

Location

United States

Related Subject Headings

  • Oxygen Consumption
  • Oxygen
  • Optics
  • Nanoparticles
  • Humans
  • Female
  • Breast Neoplasms
  • Boron
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
 

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Rickard, A. G., Zhuang, M., DeRosa, C. A., Zhang, X., Dewhirst, M. W., Fraser, C. L., & Palmer, G. M. (2020). Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells. J Biomed Opt, 25(11). https://doi.org/10.1117/1.JBO.25.11.116504
Rickard, Ashlyn G., Meng Zhuang, Christopher A. DeRosa, Xiaojie Zhang, Mark W. Dewhirst, Cassandra L. Fraser, and Gregory M. Palmer. “Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells.J Biomed Opt 25, no. 11 (November 2020). https://doi.org/10.1117/1.JBO.25.11.116504.
Rickard AG, Zhuang M, DeRosa CA, Zhang X, Dewhirst MW, Fraser CL, et al. Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells. J Biomed Opt. 2020 Nov;25(11).
Rickard, Ashlyn G., et al. “Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells.J Biomed Opt, vol. 25, no. 11, Nov. 2020. Pubmed, doi:10.1117/1.JBO.25.11.116504.
Rickard AG, Zhuang M, DeRosa CA, Zhang X, Dewhirst MW, Fraser CL, Palmer GM. Dual-emissive, oxygen-sensing boron nanoparticles quantify oxygen consumption rate in breast cancer cells. J Biomed Opt. 2020 Nov;25(11).

Published In

J Biomed Opt

DOI

EISSN

1560-2281

Publication Date

November 2020

Volume

25

Issue

11

Location

United States

Related Subject Headings

  • Oxygen Consumption
  • Oxygen
  • Optics
  • Nanoparticles
  • Humans
  • Female
  • Breast Neoplasms
  • Boron
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering