A Novel Cell-Based Multiplex Immunoassay Platform
Publication
, Journal Article
Kelsoe, G; Song, S; Manook, M; Kwun, J; Jackson, A; Knechtle, S
May 21, 2021
We describe generation of stable, fluorescence-barcoded cell lines suitable for multiplex screening of antibody to membrane proteins. The utility of this cell-based system, capable of a 256-plex cell panel, is demonstrated by flow cytometry deconvolution of barcoded cell panels expressing influenza A hemagglutinin trimers, or native human CCR2 or CCR5 multi-spanning proteins and their epitope-defining mutants. This platform may prove useful for characterizing immunity and discovering antibodies to membrane-associated proteins.
Duke Scholars
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APA
Chicago
ICMJE
MLA
NLM
Kelsoe, G., Song, S., Manook, M., Kwun, J., Jackson, A., & Knechtle, S. (2021). A Novel Cell-Based Multiplex Immunoassay Platform. https://doi.org/10.21203/rs.3.rs-464450/v1
Kelsoe, Garnett, Shengli Song, Miriam Manook, Jean Kwun, Annette Jackson, and Stuart Knechtle. “A Novel Cell-Based Multiplex Immunoassay Platform,” May 21, 2021. https://doi.org/10.21203/rs.3.rs-464450/v1.
Kelsoe G, Song S, Manook M, Kwun J, Jackson A, Knechtle S. A Novel Cell-Based Multiplex Immunoassay Platform. 2021 May 21;
Kelsoe, Garnett, et al. A Novel Cell-Based Multiplex Immunoassay Platform. May 2021. Crossref, doi:10.21203/rs.3.rs-464450/v1.
Kelsoe G, Song S, Manook M, Kwun J, Jackson A, Knechtle S. A Novel Cell-Based Multiplex Immunoassay Platform. 2021 May 21;