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Rapid test to assess the escape of SARS-CoV-2 variants of concern.

Publication ,  Journal Article
Heggestad, JT; Britton, RJ; Kinnamon, DS; Wall, SA; Joh, DY; Hucknall, AM; Olson, LB; Anderson, JG; Mazur, A; Wolfe, CR; Oguin, TH; Burke, TW ...
Published in: Sci Adv
December 3, 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are concerning in the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a rapid test, termed CoVariant-SCAN, that detects neutralizing antibodies (nAbs) capable of blocking interactions between the angiotensin-converting enzyme 2 receptor and the spike protein of wild-type (WT) SARS-CoV-2 and three other variants: B.1.1.7, B.1.351, and P.1. Using CoVariant-SCAN, we assessed neutralization/blocking of monoclonal antibodies and plasma from COVID-19–positive and vaccinated individuals. For several monoclonal antibodies and most plasma samples, neutralization against B.1.351 and P.1 variants is diminished relative to WT, while B.1.1.7 is largely cross-neutralized. We also showed that we can rapidly adapt the platform to detect nAbs against an additional variant—B.1.617.2 (Delta)—without reengineering or reoptimizing the assay. Results using CoVariant-SCAN are consistent with live virus neutralization assays and demonstrate that this easy-to-deploy test could be used to rapidly assess nAb response against multiple SARS-CoV-2 variants.

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Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

December 3, 2021

Volume

7

Issue

49

Start / End Page

eabl7682

Location

United States
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Heggestad, J. T., Britton, R. J., Kinnamon, D. S., Wall, S. A., Joh, D. Y., Hucknall, A. M., … Chilkoti, A. (2021). Rapid test to assess the escape of SARS-CoV-2 variants of concern. Sci Adv, 7(49), eabl7682. https://doi.org/10.1126/sciadv.abl7682
Heggestad, Jacob T., Rhett J. Britton, David S. Kinnamon, Simone A. Wall, Daniel Y. Joh, Angus M. Hucknall, Lyra B. Olson, et al. “Rapid test to assess the escape of SARS-CoV-2 variants of concern.Sci Adv 7, no. 49 (December 3, 2021): eabl7682. https://doi.org/10.1126/sciadv.abl7682.
Heggestad JT, Britton RJ, Kinnamon DS, Wall SA, Joh DY, Hucknall AM, et al. Rapid test to assess the escape of SARS-CoV-2 variants of concern. Sci Adv. 2021 Dec 3;7(49):eabl7682.
Heggestad, Jacob T., et al. “Rapid test to assess the escape of SARS-CoV-2 variants of concern.Sci Adv, vol. 7, no. 49, Dec. 2021, p. eabl7682. Pubmed, doi:10.1126/sciadv.abl7682.
Heggestad JT, Britton RJ, Kinnamon DS, Wall SA, Joh DY, Hucknall AM, Olson LB, Anderson JG, Mazur A, Wolfe CR, Oguin TH, Sullenger BA, Burke TW, Kraft BD, Sempowski GD, Woods CW, Chilkoti A. Rapid test to assess the escape of SARS-CoV-2 variants of concern. Sci Adv. 2021 Dec 3;7(49):eabl7682.

Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

December 3, 2021

Volume

7

Issue

49

Start / End Page

eabl7682

Location

United States