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Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells.

Publication ,  Journal Article
Yeh, C-H; Finney, J; Okada, T; Kurosaki, T; Kelsoe, G
Published in: Immunity
February 8, 2022

T follicular helper (Tfh) cells are defined by a Bcl6+CXCR5hiPD-1hi phenotype, but only a minor fraction of these reside in germinal centers (GCs). Here, we examined whether GC-resident and -nonresident Tfh cells share a common physiology and function. Fluorescently labeled, GC-resident Tfh cells in different mouse models were distinguished by low expression of CD90. CD90neg/lo GCTfh cells required antigen-specific, MHCII+ B cells to develop and stopped proliferating soon after differentiation. In contrast, nonresident, CD90hi Tfh (GCTfh-like) cells developed normally in the absence of MHCII+ B cells and proliferated continuously during primary responses. The TCR repertoires of both Tfh subsets overlapped initially but later diverged in association with dendritic cell-dependent proliferation of CD90hi GCTfh-like cells, suggestive of TCR-dependency seen also in TCR-transgenic adoptive transfer experiments. Furthermore, the transcriptomes of CD90neg/lo and CD90hi GCTfh-like cells were enriched in different functional pathways. Thus, GC-resident and nonresident Tfh cells have distinct developmental requirements and activities, implying distinct functions.

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Published In

Immunity

DOI

EISSN

1097-4180

Publication Date

February 8, 2022

Volume

55

Issue

2

Start / End Page

272 / 289.e7

Location

United States

Related Subject Headings

  • Thy-1 Antigens
  • T-Lymphocyte Subsets
  • T Follicular Helper Cells
  • Sphingosine-1-Phosphate Receptors
  • Receptors, CXCR5
  • Receptors, Antigen, T-Cell
  • Programmed Cell Death 1 Receptor
  • Mice
  • Immunology
  • Histocompatibility Antigens Class II
 

Citation

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Yeh, C.-H., Finney, J., Okada, T., Kurosaki, T., & Kelsoe, G. (2022). Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells. Immunity, 55(2), 272-289.e7. https://doi.org/10.1016/j.immuni.2021.12.015
Yeh, Chen-Hao, Joel Finney, Takaharu Okada, Tomohiro Kurosaki, and Garnett Kelsoe. “Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells.Immunity 55, no. 2 (February 8, 2022): 272-289.e7. https://doi.org/10.1016/j.immuni.2021.12.015.
Yeh, Chen-Hao, et al. “Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells.Immunity, vol. 55, no. 2, Feb. 2022, pp. 272-289.e7. Pubmed, doi:10.1016/j.immuni.2021.12.015.
Journal cover image

Published In

Immunity

DOI

EISSN

1097-4180

Publication Date

February 8, 2022

Volume

55

Issue

2

Start / End Page

272 / 289.e7

Location

United States

Related Subject Headings

  • Thy-1 Antigens
  • T-Lymphocyte Subsets
  • T Follicular Helper Cells
  • Sphingosine-1-Phosphate Receptors
  • Receptors, CXCR5
  • Receptors, Antigen, T-Cell
  • Programmed Cell Death 1 Receptor
  • Mice
  • Immunology
  • Histocompatibility Antigens Class II