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Broadly binding and functional antibodies and persisting memory B cells elicited by HIV vaccine PDPHV.

Publication ,  Journal Article
Wang, S; Yates, NL; Pollara, J; Voronin, Y; Stanfield-Oakley, S; Han, D; Hu, G; Li, W; Ferrari, G; Tomaras, GD; Lu, S
Published in: NPJ Vaccines
February 9, 2022

Since publishing our original reports on the safety and immunogenicity of a polyvalent DNA prime-protein boost HIV vaccine (PDPHV) which elicited high titer antibody responses with broad specificity, neutralizing activities to multiple HIV-1 subtypes, as well as poly-functional T cell responses, accumulated findings from other HIV vaccine studies indicated the important roles of Ig isotype distribution, Fc medicated functions and the persistence of memory immune responses which were not studied in previous PDPHV related reports. The current report provides further detailed characterization of these parameters in human volunteers receiving the PDPHV regimen. Antibody responses were assessed using IgG isotype and gp70-V1V2-binding ELISAs, peptide arrays, and antibody-dependent cellular cytotoxicity (ADCC) assays. B cell ELISPOT was used to detect gp120-specific memory B cells. Our results showed that the gp120-specific antibodies were primarily of the IgG1 isotype. HIV-1 envelope protein variable regions V1 and V2 were actively targeted by the antibodies as determined by specific binding to both peptide and V1V2-carrying scaffolds. The antibodies showed potent and broad ADCC responses. Finally, the B cell ELISPOT analysis demonstrated persistence of gp120-specific memory B cells for at least 6 months after the last dose. These data indicate that broadly reactive binding Abs and ADCC responses as well as durable gp120-specific memory B cells were elicited by the polyvalent heterologous prime-boost vaccination regimens and showed great promise as a candidate HIV vaccine.

Duke Scholars

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Published In

NPJ Vaccines

DOI

EISSN

2059-0105

Publication Date

February 9, 2022

Volume

7

Issue

1

Start / End Page

18

Location

England

Related Subject Headings

  • 3207 Medical microbiology
  • 3204 Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, S., Yates, N. L., Pollara, J., Voronin, Y., Stanfield-Oakley, S., Han, D., … Lu, S. (2022). Broadly binding and functional antibodies and persisting memory B cells elicited by HIV vaccine PDPHV. NPJ Vaccines, 7(1), 18. https://doi.org/10.1038/s41541-022-00441-9
Wang, Shixia, Nicole L. Yates, Justin Pollara, Yegor Voronin, Sherry Stanfield-Oakley, Dong Han, Guangnan Hu, et al. “Broadly binding and functional antibodies and persisting memory B cells elicited by HIV vaccine PDPHV.NPJ Vaccines 7, no. 1 (February 9, 2022): 18. https://doi.org/10.1038/s41541-022-00441-9.
Wang S, Yates NL, Pollara J, Voronin Y, Stanfield-Oakley S, Han D, et al. Broadly binding and functional antibodies and persisting memory B cells elicited by HIV vaccine PDPHV. NPJ Vaccines. 2022 Feb 9;7(1):18.
Wang, Shixia, et al. “Broadly binding and functional antibodies and persisting memory B cells elicited by HIV vaccine PDPHV.NPJ Vaccines, vol. 7, no. 1, Feb. 2022, p. 18. Pubmed, doi:10.1038/s41541-022-00441-9.
Wang S, Yates NL, Pollara J, Voronin Y, Stanfield-Oakley S, Han D, Hu G, Li W, Ferrari G, Tomaras GD, Lu S. Broadly binding and functional antibodies and persisting memory B cells elicited by HIV vaccine PDPHV. NPJ Vaccines. 2022 Feb 9;7(1):18.

Published In

NPJ Vaccines

DOI

EISSN

2059-0105

Publication Date

February 9, 2022

Volume

7

Issue

1

Start / End Page

18

Location

England

Related Subject Headings

  • 3207 Medical microbiology
  • 3204 Immunology