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The role of Meteorin-like in skeletal development and bone fracture healing.

Publication ,  Journal Article
Huang, R; Balu, AR; Molitoris, KH; White, JP; Robling, AG; Ayturk, UM; Baht, GS
Published in: J Orthop Res
November 2022

Meteorin-like protein (Metrnl), homologous to the initially identified neurotrophic factor Meteorin, is a secreted, multifunctional protein. Here we used mouse models to investigate Metrnl's role in skeletal development and bone fracture healing. During development Metrnl was expressed in the perichondrium and primary ossification center. In neonates, single cell RNA-seq of diaphyseal bone demonstrated strongest expression of Metrnl transcript by osteoblasts. In vitro, Metrnl was osteoinductive, increasing osteoblast differentiation and mineralization in tissue culture models. In vivo, loss of Metrnl expression resulted in no change in skeletal metrics in utero, at birth, or during postnatal growth. Six-week-old Metrnl-null mice displayed similar body length, body weight, tibial length, femoral length, BV/TV, trabecular number, trabecular thickness, and cortical thickness as littermate controls. In 4-month-old mice, lack of Metrnl expression did not change structural stiffness, ultimate force, or energy to fracture of femora under 3-point-bending. Last, we investigated the role of Metrnl in bone fracture healing. Metrnl expression increased in response to tibial injury, however, loss of Metrnl expression did not affect the amount of bone deposited within the healing tissue nor did it change the structural parameters of healing tissue. This work identifies Metrnl as a dispensable molecule for skeletal development. However, the osteoinductive capabilities of Metrnl may be utilized to modulate osteoblast differentiation in cell-based orthopedic therapies.

Duke Scholars

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

November 2022

Volume

40

Issue

11

Start / End Page

2510 / 2521

Location

United States

Related Subject Headings

  • Osteoblasts
  • Orthopedics
  • Nerve Growth Factors
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Fracture Healing
  • Animals
  • 4207 Sports science and exercise
  • 4003 Biomedical engineering
 

Citation

APA
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ICMJE
MLA
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Huang, R., Balu, A. R., Molitoris, K. H., White, J. P., Robling, A. G., Ayturk, U. M., & Baht, G. S. (2022). The role of Meteorin-like in skeletal development and bone fracture healing. J Orthop Res, 40(11), 2510–2521. https://doi.org/10.1002/jor.25286
Huang, Rong, Abhinav R. Balu, Kristin H. Molitoris, James P. White, Alexander G. Robling, Ugur M. Ayturk, and Gurpreet S. Baht. “The role of Meteorin-like in skeletal development and bone fracture healing.J Orthop Res 40, no. 11 (November 2022): 2510–21. https://doi.org/10.1002/jor.25286.
Huang R, Balu AR, Molitoris KH, White JP, Robling AG, Ayturk UM, et al. The role of Meteorin-like in skeletal development and bone fracture healing. J Orthop Res. 2022 Nov;40(11):2510–21.
Huang, Rong, et al. “The role of Meteorin-like in skeletal development and bone fracture healing.J Orthop Res, vol. 40, no. 11, Nov. 2022, pp. 2510–21. Pubmed, doi:10.1002/jor.25286.
Huang R, Balu AR, Molitoris KH, White JP, Robling AG, Ayturk UM, Baht GS. The role of Meteorin-like in skeletal development and bone fracture healing. J Orthop Res. 2022 Nov;40(11):2510–2521.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

November 2022

Volume

40

Issue

11

Start / End Page

2510 / 2521

Location

United States

Related Subject Headings

  • Osteoblasts
  • Orthopedics
  • Nerve Growth Factors
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Fracture Healing
  • Animals
  • 4207 Sports science and exercise
  • 4003 Biomedical engineering