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Recall of B cell memory depends on relative locations of prime and boost immunization.

Publication ,  Journal Article
Kuraoka, M; Yeh, C-H; Bajic, G; Kotaki, R; Song, S; Windsor, I; Harrison, SC; Kelsoe, G
Published in: Sci Immunol
May 6, 2022

Immunization or microbial infection can establish long-term B cell memory not only systemically but also locally. Evidence has suggested that local B cell memory contributes to early local plasmacytic responses after secondary challenge. However, it is unclear whether locality of immunization plays any role in memory B cell participation in recall germinal centers (GCs), which is essential for updating their B cell antigen receptors (BCRs). Using single B cell culture and fate mapping, we have characterized BCR repertoires in recall GCs after boost immunizations at sites local or distal to the priming. Local boosts with homologous antigen recruit the progeny of primary GC B cells to recall GCs more efficiently than do distal boosts. Recall GCs elicited by local boosts contain significantly more B cells with elevated levels of immunoglobulin (Ig) mutation and higher avidity BCRs. This local preference is unaffected by blocking CD40:CD154 interaction to terminate active, GC responses. Local boosts with heterologous antigens elicit secondary GCs with B cell populations enriched for cross-reactivity to the prime and boost antigens; in contrast, cross-reactive GC B cells are rare after distal boosts. Our results suggest that local B cell memory is retained in the form of memory B cells, GC B cells, and GC phenotype B cells that are independent of organized GC structures and that these persistent "primed B cells" contribute to recall GC responses at local sites. Our findings indicate the importance of locality in humoral immunity and inform serial vaccination strategies for evolving viruses.

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Published In

Sci Immunol

DOI

EISSN

2470-9468

Publication Date

May 6, 2022

Volume

7

Issue

71

Start / End Page

eabn5311

Location

United States

Related Subject Headings

  • Vaccination
  • Immunization
  • Immunity, Humoral
  • Germinal Center
  • B-Lymphocytes
  • Antigens
  • 3204 Immunology
  • 3202 Clinical sciences
 

Citation

APA
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MLA
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Kuraoka, M., Yeh, C.-H., Bajic, G., Kotaki, R., Song, S., Windsor, I., … Kelsoe, G. (2022). Recall of B cell memory depends on relative locations of prime and boost immunization. Sci Immunol, 7(71), eabn5311. https://doi.org/10.1126/sciimmunol.abn5311
Kuraoka, Masayuki, Chen-Hao Yeh, Goran Bajic, Ryutaro Kotaki, Shengli Song, Ian Windsor, Stephen C. Harrison, and Garnett Kelsoe. “Recall of B cell memory depends on relative locations of prime and boost immunization.Sci Immunol 7, no. 71 (May 6, 2022): eabn5311. https://doi.org/10.1126/sciimmunol.abn5311.
Kuraoka M, Yeh C-H, Bajic G, Kotaki R, Song S, Windsor I, et al. Recall of B cell memory depends on relative locations of prime and boost immunization. Sci Immunol. 2022 May 6;7(71):eabn5311.
Kuraoka, Masayuki, et al. “Recall of B cell memory depends on relative locations of prime and boost immunization.Sci Immunol, vol. 7, no. 71, May 2022, p. eabn5311. Pubmed, doi:10.1126/sciimmunol.abn5311.
Kuraoka M, Yeh C-H, Bajic G, Kotaki R, Song S, Windsor I, Harrison SC, Kelsoe G. Recall of B cell memory depends on relative locations of prime and boost immunization. Sci Immunol. 2022 May 6;7(71):eabn5311.

Published In

Sci Immunol

DOI

EISSN

2470-9468

Publication Date

May 6, 2022

Volume

7

Issue

71

Start / End Page

eabn5311

Location

United States

Related Subject Headings

  • Vaccination
  • Immunization
  • Immunity, Humoral
  • Germinal Center
  • B-Lymphocytes
  • Antigens
  • 3204 Immunology
  • 3202 Clinical sciences