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High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer.

Publication ,  Journal Article
Chen, M; Jiang, L; Cook, C; Zeng, Y; Vu, T; Chen, R; Lu, G; Yang, W; Hoffmann, U; Zhou, Q; Yao, J
Published in: Photoacoustics
December 2022

Combining focused optical excitation and high-frequency ultrasound detection, optical-resolution photoacoustic microscopy (OR-PAM) can provide micrometer-level spatial resolution with millimeter-level penetration depth and has been employed in a variety of biomedical applications. However, it remains a challenge for OR-PAM to achieve a high imaging speed and a large field of view at the same time. In this work, we report a new approach to implement high-speed wide-field OR-PAM, using a cylindrically-focused transparent ultrasound transducer (CFT-UT). The CFT-UT is made of transparent lithium niobate coated with indium-tin-oxide as electrodes. A transparent cylindrical lens is attached to the transducer surface to provide an acoustic focal line with a length of 9 mm. The excitation light can pass directly through the CFT-UT from the above and thus enables a reflection imaging mode. High-speed imaging is achieved by fast optical scanning of the focused excitation light along the CFT-UT focal line. With the confocal alignment of the optical excitation and acoustic detection, a relatively high detection sensitivity is maintained over the entire scanning range. The CFT-UT-based OR-PAM system has achieved a cross-sectional frame rate of 500 Hz over the scanning range of 9 mm. We have characterized the system's performance on phantoms and demonstrated its application on small animal models in vivo. We expect the new CFT-UT-based OR-PAM will find matched biomedical applications that need high imaging speed over a large field of view.

Duke Scholars

Published In

Photoacoustics

DOI

ISSN

2213-5979

Publication Date

December 2022

Volume

28

Start / End Page

100417

Location

Germany
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, M., Jiang, L., Cook, C., Zeng, Y., Vu, T., Chen, R., … Yao, J. (2022). High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer. Photoacoustics, 28, 100417. https://doi.org/10.1016/j.pacs.2022.100417
Chen, Maomao, Laiming Jiang, Clare Cook, Yushun Zeng, Tri Vu, Ruimin Chen, Gengxi Lu, et al. “High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer.Photoacoustics 28 (December 2022): 100417. https://doi.org/10.1016/j.pacs.2022.100417.
Chen M, Jiang L, Cook C, Zeng Y, Vu T, Chen R, et al. High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer. Photoacoustics. 2022 Dec;28:100417.
Chen, Maomao, et al. “High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer.Photoacoustics, vol. 28, Dec. 2022, p. 100417. Pubmed, doi:10.1016/j.pacs.2022.100417.
Chen M, Jiang L, Cook C, Zeng Y, Vu T, Chen R, Lu G, Yang W, Hoffmann U, Zhou Q, Yao J. High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer. Photoacoustics. 2022 Dec;28:100417.
Journal cover image

Published In

Photoacoustics

DOI

ISSN

2213-5979

Publication Date

December 2022

Volume

28

Start / End Page

100417

Location

Germany