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A Clinical Study to Assess Diffuse Reflectance Spectroscopy with an Auto-Calibrated, Pressure-Sensing Optical Probe in Head and Neck Cancer.

Publication ,  Journal Article
Rickard, AG; Mikati, H; Mansourati, A; Stevenson, D; Krieger, M; Rocke, D; Esclamado, R; Dewhirst, MW; Ramanujam, N; Lee, WT; Palmer, GM
Published in: Curr Oncol
February 24, 2023

Diffuse reflectance spectroscopy (DRS) is a powerful tool for quantifying optical and physiological tissue properties such as hemoglobin oxygen saturation and vascularity. DRS is increasingly used clinically for distinguishing cancerous lesions from normal tissue. However, its widespread clinical acceptance is still limited due to uncontrolled probe-tissue interface pressure that influences reproducibility and introduces operator-dependent results. In this clinical study, we assessed and validated a pressure-sensing and automatic self-calibration DRS in patients with suspected head and neck squamous cell carcinoma (HNSCC). The clinical study enrolled nineteen patients undergoing HNSCC surgical biopsy procedures. Patients consented to evaluation of this improved DRS system during surgery. For each patient, we obtained 10 repeated measurements on one tumor site and one distant normal location. Using a Monte Carlo-based model, we extracted the hemoglobin saturation data along with total hemoglobin content and scattering properties. A total of twelve cancer tissue samples from HNSCC patients and fourteen normal tissues were analyzed. A linear mixed effects model tested for significance between repeated measurements and compared tumor versus normal tissue. These results demonstrate that cancerous tissues have a significantly lower hemoglobin saturation compared to normal controls (p < 0.001), which may be reflective of tumor hypoxia. In addition, there were minimal changes over time upon probe placement and repeated measurement, indicating that the pressure-induced changes were minimal and repeated measurements did not differ significantly from the initial value. This study demonstrates the feasibility of conducting optical spectroscopy measurements on intact lesions prior to removal during HNSCC procedures, and established that this probe provides diagnostically-relevant physiologic information that may impact further treatment.

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Published In

Curr Oncol

DOI

EISSN

1718-7729

Publication Date

February 24, 2023

Volume

30

Issue

3

Start / End Page

2751 / 2760

Location

Switzerland

Related Subject Headings

  • Squamous Cell Carcinoma of Head and Neck
  • Spectrum Analysis
  • Reproducibility of Results
  • Oncology & Carcinogenesis
  • Humans
  • Hemoglobins
  • Head and Neck Neoplasms
  • 3211 Oncology and carcinogenesis
  • 1112 Oncology and Carcinogenesis
 

Citation

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Rickard, A. G., Mikati, H., Mansourati, A., Stevenson, D., Krieger, M., Rocke, D., … Palmer, G. M. (2023). A Clinical Study to Assess Diffuse Reflectance Spectroscopy with an Auto-Calibrated, Pressure-Sensing Optical Probe in Head and Neck Cancer. Curr Oncol, 30(3), 2751–2760. https://doi.org/10.3390/curroncol30030208
Rickard, Ashlyn G., Husam Mikati, Antoine Mansourati, Daniel Stevenson, Marlee Krieger, Daniel Rocke, Ramon Esclamado, et al. “A Clinical Study to Assess Diffuse Reflectance Spectroscopy with an Auto-Calibrated, Pressure-Sensing Optical Probe in Head and Neck Cancer.Curr Oncol 30, no. 3 (February 24, 2023): 2751–60. https://doi.org/10.3390/curroncol30030208.
Rickard AG, Mikati H, Mansourati A, Stevenson D, Krieger M, Rocke D, et al. A Clinical Study to Assess Diffuse Reflectance Spectroscopy with an Auto-Calibrated, Pressure-Sensing Optical Probe in Head and Neck Cancer. Curr Oncol. 2023 Feb 24;30(3):2751–60.
Rickard, Ashlyn G., et al. “A Clinical Study to Assess Diffuse Reflectance Spectroscopy with an Auto-Calibrated, Pressure-Sensing Optical Probe in Head and Neck Cancer.Curr Oncol, vol. 30, no. 3, Feb. 2023, pp. 2751–60. Pubmed, doi:10.3390/curroncol30030208.
Rickard AG, Mikati H, Mansourati A, Stevenson D, Krieger M, Rocke D, Esclamado R, Dewhirst MW, Ramanujam N, Lee WT, Palmer GM. A Clinical Study to Assess Diffuse Reflectance Spectroscopy with an Auto-Calibrated, Pressure-Sensing Optical Probe in Head and Neck Cancer. Curr Oncol. 2023 Feb 24;30(3):2751–2760.

Published In

Curr Oncol

DOI

EISSN

1718-7729

Publication Date

February 24, 2023

Volume

30

Issue

3

Start / End Page

2751 / 2760

Location

Switzerland

Related Subject Headings

  • Squamous Cell Carcinoma of Head and Neck
  • Spectrum Analysis
  • Reproducibility of Results
  • Oncology & Carcinogenesis
  • Humans
  • Hemoglobins
  • Head and Neck Neoplasms
  • 3211 Oncology and carcinogenesis
  • 1112 Oncology and Carcinogenesis