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Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition.

Publication ,  Journal Article
Huang, G; Cheng, Z; Hildebrand, A; Wang, C; Cimini, M; Roy, R; Lucchese, AM; Benedict, C; Mallaredy, V; Magadum, A; Joladarashi, D; Thej, C ...
Published in: Theranostics
2022

Background and Purpose: Myocardial infarction (MI) in diabetic patients results in higher mortality and morbidity. We and others have previously shown that bone marrow-endothelial progenitor cells (EPCs) promote cardiac neovascularization and attenuate ischemic injury. Lately, small extracellular vesicles (EVs) have emerged as major paracrine effectors mediating the benefits of stem cell therapy. Modest clinical outcomes of autologous cell-based therapies suggest diabetes-induced EPC dysfunction and may also reflect their EV derivatives. Moreover, studies suggest that post-translational histone modifications promote diabetes-induced vascular dysfunctions. Therefore, we tested the hypothesis that diabetic EPC-EVs may lose their post-injury cardiac reparative function by modulating histone modification in endothelial cells (ECs). Methods: We collected EVs from the culture medium of EPCs isolated from non-diabetic (db/+) and diabetic (db/db) mice and examined their effects on recipient ECs and cardiomyocytes in vitro, and their reparative function in permanent ligation of left anterior descending (LAD) coronary artery and ischemia/reperfusion (I/R) myocardial ischemic injuries in vivo. Results: Compared to db/+ EPC-EVs, db/db EPC-EVs promoted EC and cardiomyocyte apoptosis and repressed tube-forming capacity of ECs. In vivo, db/db EPC-EVs depressed cardiac function, reduced capillary density, and increased fibrosis compared to db/+ EPC-EV treatments after MI. Moreover, in the I/R MI model, db/+ EPC-EV-mediated acute cardio-protection was lost with db/db EPC-EVs, and db/db EPC-EVs increased immune cell infiltration, infarct area, and plasma cardiac troponin-I. Mechanistically, histone 3 lysine 9 acetylation (H3K9Ac) was significantly decreased in cardiac ECs treated with db/db EPC-EVs compared to db/+ EPC-EVs. The H3K9Ac chromatin immunoprecipitation sequencing (ChIP-Seq) results further revealed that db/db EPC-EVs reduced H3K9Ac level on angiogenic, cell survival, and proliferative genes in cardiac ECs. We found that the histone deacetylase (HDAC) inhibitor, valproic acid (VPA), partly restored diabetic EPC-EV-impaired H3K9Ac levels, tube formation and viability of ECs, and enhanced cell survival and proliferative genes, Pdgfd and Sox12, expression. Moreover, we observed that VPA treatment improved db/db EPC-mediated post-MI cardiac repair and functions. Conclusions: Our findings unravel that diabetes impairs EPC-EV reparative function in the ischemic heart, at least partially, through HDACs-mediated H3K9Ac downregulation leading to transcriptional suppression of angiogenic, proliferative and cell survival genes in recipient cardiac ECs. Thus, HDAC inhibitors may potentially be used to restore the function of diabetic EPC and other stem cells for autologous cell therapy applications.

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Published In

Theranostics

DOI

EISSN

1838-7640

Publication Date

2022

Volume

12

Issue

9

Start / End Page

4415 / 4430

Location

Australia

Related Subject Headings

  • SOXC Transcription Factors
  • Myocytes, Cardiac
  • Myocardial Infarction
  • Mice
  • Humans
  • Histones
  • Extracellular Vesicles
  • Endothelial Progenitor Cells
  • Diabetes Mellitus
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huang, G., Cheng, Z., Hildebrand, A., Wang, C., Cimini, M., Roy, R., … Kishore, R. (2022). Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition. Theranostics, 12(9), 4415–4430. https://doi.org/10.7150/thno.70821
Huang, Grace, Zhongjian Cheng, Alycia Hildebrand, Chunlin Wang, Maria Cimini, Rakija Roy, Anna Maria Lucchese, et al. “Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition.Theranostics 12, no. 9 (2022): 4415–30. https://doi.org/10.7150/thno.70821.
Huang G, Cheng Z, Hildebrand A, Wang C, Cimini M, Roy R, et al. Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition. Theranostics. 2022;12(9):4415–30.
Huang, Grace, et al. “Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition.Theranostics, vol. 12, no. 9, 2022, pp. 4415–30. Pubmed, doi:10.7150/thno.70821.
Huang G, Cheng Z, Hildebrand A, Wang C, Cimini M, Roy R, Lucchese AM, Benedict C, Mallaredy V, Magadum A, Joladarashi D, Thej C, Gonzalez C, Trungcao M, Garikipati VNS, Elrod JW, Koch WJ, Kishore R. Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition. Theranostics. 2022;12(9):4415–4430.

Published In

Theranostics

DOI

EISSN

1838-7640

Publication Date

2022

Volume

12

Issue

9

Start / End Page

4415 / 4430

Location

Australia

Related Subject Headings

  • SOXC Transcription Factors
  • Myocytes, Cardiac
  • Myocardial Infarction
  • Mice
  • Humans
  • Histones
  • Extracellular Vesicles
  • Endothelial Progenitor Cells
  • Diabetes Mellitus
  • Animals