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Trophoblast organoids with physiological polarity model placental structure and function.

Publication ,  Journal Article
Yang, L; Liang, P; Yang, H; Coyne, CB
Published in: J Cell Sci
March 1, 2024

Human trophoblast organoids (TOs) are a three-dimensional ex vivo culture model that can be used to study various aspects of placental development, physiology and pathology. However, standard culturing of TOs does not recapitulate the cellular orientation of chorionic villi in vivo given that the multi-nucleated syncytiotrophoblast (STB) develops largely within the inner facing surfaces of these organoids (STBin). Here, we developed a method to culture TOs under conditions that recapitulate the cellular orientation of chorionic villi in vivo. We show that culturing STBin TOs in suspension with gentle agitation leads to the development of TOs containing the STB on the outer surface (STBout). Using membrane capacitance measurements, we determined that the outermost surface of STBout organoids contain large syncytia comprising >50 nuclei, whereas STBin organoids contain small syncytia (<10 nuclei) and mononuclear cells. The growth of TOs under conditions that mimic the cellular orientation of chorionic villi in vivo thus allows for the study of a variety of aspects of placental biology under physiological conditions.

Duke Scholars

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Published In

J Cell Sci

DOI

EISSN

1477-9137

Publication Date

March 1, 2024

Volume

137

Issue

5

Location

England

Related Subject Headings

  • Trophoblasts
  • Pregnancy
  • Placenta
  • Organoids
  • Humans
  • Giant Cells
  • Female
  • Developmental Biology
  • Cell Nucleus
  • 3101 Biochemistry and cell biology
 

Citation

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Yang, L., Liang, P., Yang, H., & Coyne, C. B. (2024). Trophoblast organoids with physiological polarity model placental structure and function. J Cell Sci, 137(5). https://doi.org/10.1242/jcs.261528
Yang, Liheng, Pengfei Liang, Huanghe Yang, and Carolyn B. Coyne. “Trophoblast organoids with physiological polarity model placental structure and function.J Cell Sci 137, no. 5 (March 1, 2024). https://doi.org/10.1242/jcs.261528.
Yang L, Liang P, Yang H, Coyne CB. Trophoblast organoids with physiological polarity model placental structure and function. J Cell Sci. 2024 Mar 1;137(5).
Yang, Liheng, et al. “Trophoblast organoids with physiological polarity model placental structure and function.J Cell Sci, vol. 137, no. 5, Mar. 2024. Pubmed, doi:10.1242/jcs.261528.
Yang L, Liang P, Yang H, Coyne CB. Trophoblast organoids with physiological polarity model placental structure and function. J Cell Sci. 2024 Mar 1;137(5).
Journal cover image

Published In

J Cell Sci

DOI

EISSN

1477-9137

Publication Date

March 1, 2024

Volume

137

Issue

5

Location

England

Related Subject Headings

  • Trophoblasts
  • Pregnancy
  • Placenta
  • Organoids
  • Humans
  • Giant Cells
  • Female
  • Developmental Biology
  • Cell Nucleus
  • 3101 Biochemistry and cell biology