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Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio.

Publication ,  Journal Article
Kozal, JS; Jayasundara, N; Massarsky, A; Lindberg, CD; Oliveri, AN; Cooper, EM; Levin, ED; Meyer, JN; Giulio, RTD
Published in: Aquat Toxicol
October 2023

The potential for polycyclic aromatic hydrocarbons (PAHs) to have adverse effects that persist across generations is an emerging concern for human and wildlife health. This study evaluated the role of mitochondria, which are maternally inherited, in the cross-generational toxicity of benzo(a)pyrene (BaP), a model PAH and known mitochondrial toxicant. Mature female zebrafish (F0) were fed diets containing 0, 12.5, 125, or 1250 μg BaP/g at a feed rate of 1% body weight twice/day for 21 days. These females were bred with unexposed males, and the embryos (F1) were collected for subsequent analyses. Maternally-exposed embryos exhibited altered mitochondrial function and metabolic partitioning (i.e. the portion of respiration attributable to different cellular processes), as evidenced by in vivo oxygen consumption rates (OCRs). F1 embryos had lower basal and mitochondrial respiration and ATP turnover-mediated OCR, and increased proton leak and reserve capacity. Reductions in mitochondrial DNA (mtDNA) copy number, increases in mtDNA damage, and alterations in biomarkers of oxidative stress were also found in maternally-exposed embryos. Notably, the mitochondrial effects in offspring occurred largely in the absence of effects in maternal ovaries, suggesting that PAH-induced mitochondrial dysfunction may manifest in subsequent generations. Maternally-exposed larvae also displayed swimming hypoactivity. The lowest observed effect level (LOEL) for maternal BaP exposure causing mitochondrial effects in offspring was 12.5 µg BaP/g diet (nominally equivalent to 250 ng BaP/g fish). It was concluded that maternal BaP exposure can cause significant mitochondrial impairments in offspring.

Duke Scholars

Published In

Aquat Toxicol

DOI

EISSN

1879-1514

Publication Date

October 2023

Volume

263

Start / End Page

106658

Location

Netherlands

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Toxicology
  • Oxygen Consumption
  • Oxidative Stress
  • Mitochondria
  • Maternal Exposure
  • Male
  • Larva
  • Female
 

Citation

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Kozal, J. S., Jayasundara, N., Massarsky, A., Lindberg, C. D., Oliveri, A. N., Cooper, E. M., … Giulio, R. T. D. (2023). Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio. Aquat Toxicol, 263, 106658. https://doi.org/10.1016/j.aquatox.2023.106658
Kozal, Jordan S., Nishad Jayasundara, Andrey Massarsky, Casey D. Lindberg, Anthony N. Oliveri, Ellen M. Cooper, Edward D. Levin, Joel N. Meyer, and Richard T Di Giulio. “Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio.Aquat Toxicol 263 (October 2023): 106658. https://doi.org/10.1016/j.aquatox.2023.106658.
Kozal JS, Jayasundara N, Massarsky A, Lindberg CD, Oliveri AN, Cooper EM, et al. Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio. Aquat Toxicol. 2023 Oct;263:106658.
Kozal, Jordan S., et al. “Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio.Aquat Toxicol, vol. 263, Oct. 2023, p. 106658. Pubmed, doi:10.1016/j.aquatox.2023.106658.
Kozal JS, Jayasundara N, Massarsky A, Lindberg CD, Oliveri AN, Cooper EM, Levin ED, Meyer JN, Giulio RTD. Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio. Aquat Toxicol. 2023 Oct;263:106658.
Journal cover image

Published In

Aquat Toxicol

DOI

EISSN

1879-1514

Publication Date

October 2023

Volume

263

Start / End Page

106658

Location

Netherlands

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Toxicology
  • Oxygen Consumption
  • Oxidative Stress
  • Mitochondria
  • Maternal Exposure
  • Male
  • Larva
  • Female