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Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020.

Publication ,  Journal Article
Gin, TE; Petzold, EA; Uthappa, DM; Neighbors, CE; Borough, AR; Gin, C; Lashnits, E; Sempowski, GD; Denny, T; Bienzle, D; Weese, JS; Woods, CW ...
Published in: PeerJ
2023

We collected oral and/or rectal swabs and serum from dogs and cats living in homes with SARS-CoV-2-PCR-positive persons for SARS-CoV-2 PCR and serology testing. Pre-COVID-19 serum samples from dogs and cats were used as negative controls, and samples were tested in duplicate at different timepoints. Raw ELISA results scrutinized relative to known negative samples suggested that cut-offs for IgG seropositivity may require adjustment relative to previously proposed values, while proposed cut-offs for IgM require more extensive validation. A small number of pet dogs (2/43, 4.7%) and one cat (1/21, 4.8%) were positive for SARS-CoV-2 RNA, and 28.6 and 37.5% of cats and dogs were positive for anti-SARS-CoV-2 IgG, respectively.

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Published In

PeerJ

DOI

EISSN

2167-8359

Publication Date

2023

Volume

11

Start / End Page

e16310

Location

United States

Related Subject Headings

  • SARS-CoV-2
  • RNA, Viral
  • Pets
  • North Carolina
  • Immunoglobulin G
  • Dogs
  • Dog Diseases
  • Cats
  • Cat Diseases
  • COVID-19
 

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Gin, T. E., Petzold, E. A., Uthappa, D. M., Neighbors, C. E., Borough, A. R., Gin, C., … Woods, C. W. (2023). Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020. PeerJ, 11, e16310. https://doi.org/10.7717/peerj.16310
Gin, Taylor E., Elizabeth A. Petzold, Diya M. Uthappa, Coralei E. Neighbors, Anna R. Borough, Craig Gin, Erin Lashnits, et al. “Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020.PeerJ 11 (2023): e16310. https://doi.org/10.7717/peerj.16310.
Gin TE, Petzold EA, Uthappa DM, Neighbors CE, Borough AR, Gin C, Lashnits E, Sempowski GD, Denny T, Bienzle D, Weese JS, Callahan BJ, Woods CW. Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020. PeerJ. 2023;11:e16310.

Published In

PeerJ

DOI

EISSN

2167-8359

Publication Date

2023

Volume

11

Start / End Page

e16310

Location

United States

Related Subject Headings

  • SARS-CoV-2
  • RNA, Viral
  • Pets
  • North Carolina
  • Immunoglobulin G
  • Dogs
  • Dog Diseases
  • Cats
  • Cat Diseases
  • COVID-19