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Recognition of U1 and U2 small nuclear RNAs can be altered by a 5-amino-acid segment in the U2 small nuclear ribonucleoprotein particle (snRNP) B" protein and through interactions with U2 snRNP-A' protein.

Publication ,  Journal Article
Bentley, RC; Keene, JD
Published in: Mol Cell Biol
April 1991

We have investigated the sequence elements influencing RNA recognition in two closely related small nuclear ribonucleoprotein particle (snRNP) proteins, U1 snRNP-A and U2 snRNP-B". A 5-amino-acid segment in the RNA-binding domain of the U2 snRNP-B" protein was found to confer U2 RNA recognition when substituted into the corresponding position in the U1 snRNP-A protein. In addition, B", but not A, was found to require the U2 snRNP-A' protein as an accessory factor for high-affinity binding to U2 RNA. The pentamer segment in B" that conferred U2 RNA recognition was not sufficient to allow the A' enhancement of U2 RNA binding by B", thus implicating other sequences in this protein-protein interaction. Sequence elements involved in these interactions have been localized to variable loops of the RNA-binding domain as determined by nuclear magnetic resonance spectroscopy (D. Hoffman, C.C. Query, B. Golden, S.W. White, and J.D. Keene, Proc. Natl. Acad. Sci. USA, in press). These findings suggest a role for accessory proteins in the formation of RNP complexes and pinpoint amino acid sequences that affect the specificity of RNA recognition in two members of a large family of proteins involved in RNA processing.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

April 1991

Volume

11

Issue

4

Start / End Page

1829 / 1839

Location

United States

Related Subject Headings

  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins
  • RNA, Small Nuclear
  • Nucleic Acid Conformation
  • Mutation
  • Molecular Sequence Data
  • Humans
  • Hela Cells
  • HeLa Cells
  • Developmental Biology
 

Citation

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Bentley, R. C., and J. D. Keene. “Recognition of U1 and U2 small nuclear RNAs can be altered by a 5-amino-acid segment in the U2 small nuclear ribonucleoprotein particle (snRNP) B" protein and through interactions with U2 snRNP-A' protein.Mol Cell Biol 11, no. 4 (April 1991): 1829–39. https://doi.org/10.1128/mcb.11.4.1829-1839.1991.
Bentley, R. C., and J. D. Keene. “Recognition of U1 and U2 small nuclear RNAs can be altered by a 5-amino-acid segment in the U2 small nuclear ribonucleoprotein particle (snRNP) B" protein and through interactions with U2 snRNP-A' protein.Mol Cell Biol, vol. 11, no. 4, Apr. 1991, pp. 1829–39. Pubmed, doi:10.1128/mcb.11.4.1829-1839.1991.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

April 1991

Volume

11

Issue

4

Start / End Page

1829 / 1839

Location

United States

Related Subject Headings

  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins
  • RNA, Small Nuclear
  • Nucleic Acid Conformation
  • Mutation
  • Molecular Sequence Data
  • Humans
  • Hela Cells
  • HeLa Cells
  • Developmental Biology