Skip to main content
Journal cover image

RNA interference using boranophosphate siRNAs: structure-activity relationships.

Publication ,  Journal Article
Hall, AHS; Wan, J; Shaughnessy, EE; Ramsay Shaw, B; Alexander, KA
Published in: Nucleic Acids Res
2004

In RNA interference (RNAi), short double-stranded RNA (known as siRNA) inhibits expression from homologous genes. Clinical or pre-clinical use of siRNAs is likely to require stabilizing modifications because of the prevalence of intracellular and extracellular nucleases. In order to examine the effect of modification on siRNA efficacy and stability, we developed a new method for synthesizing stereoregular boranophosphate siRNAs. This work demonstrates that boranophosphate siRNAs are consistently more effective than siRNAs with the widely used phosphorothioate modification. Furthermore, boranophosphate siRNAs are frequently more active than native siRNA if the center of the antisense strand is not modified. Boranophosphate modification also increases siRNA potency. The finding that boranophosphate siRNAs are at least ten times more nuclease resistant than unmodified siRNAs may explain some of the positive effects of boranophosphate modification. The biochemical properties of boranophosphate siRNAs make them promising candidates for an RNAi-based therapeutic.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

2004

Volume

32

Issue

20

Start / End Page

5991 / 6000

Location

England

Related Subject Headings

  • Thionucleotides
  • Structure-Activity Relationship
  • Ribonucleases
  • RNA, Small Interfering
  • RNA, Messenger
  • RNA Interference
  • Phosphates
  • Humans
  • Hela Cells
  • HeLa Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hall, A. H. S., Wan, J., Shaughnessy, E. E., Ramsay Shaw, B., & Alexander, K. A. (2004). RNA interference using boranophosphate siRNAs: structure-activity relationships. Nucleic Acids Res, 32(20), 5991–6000. https://doi.org/10.1093/nar/gkh936
Hall, Allison H. S., Jing Wan, Erin E. Shaughnessy, Barbara Ramsay Shaw, and Kenneth A. Alexander. “RNA interference using boranophosphate siRNAs: structure-activity relationships.Nucleic Acids Res 32, no. 20 (2004): 5991–6000. https://doi.org/10.1093/nar/gkh936.
Hall AHS, Wan J, Shaughnessy EE, Ramsay Shaw B, Alexander KA. RNA interference using boranophosphate siRNAs: structure-activity relationships. Nucleic Acids Res. 2004;32(20):5991–6000.
Hall, Allison H. S., et al. “RNA interference using boranophosphate siRNAs: structure-activity relationships.Nucleic Acids Res, vol. 32, no. 20, 2004, pp. 5991–6000. Pubmed, doi:10.1093/nar/gkh936.
Hall AHS, Wan J, Shaughnessy EE, Ramsay Shaw B, Alexander KA. RNA interference using boranophosphate siRNAs: structure-activity relationships. Nucleic Acids Res. 2004;32(20):5991–6000.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

2004

Volume

32

Issue

20

Start / End Page

5991 / 6000

Location

England

Related Subject Headings

  • Thionucleotides
  • Structure-Activity Relationship
  • Ribonucleases
  • RNA, Small Interfering
  • RNA, Messenger
  • RNA Interference
  • Phosphates
  • Humans
  • Hela Cells
  • HeLa Cells