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Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation.

Publication ,  Journal Article
Billon, N; Carlisi, D; Datto, MB; van Grunsven, LA; Watt, A; Wang, XF; Rudkin, BB
Published in: Oncogene
May 6, 1999

Addition of nerve growth factor (NGF) to PC12 cells promotes neuronal differentiation while inhibiting cell proliferation. In order to understand how NGF exerts its antimitogenic effect during differentiation, we have studied the mechanism by which this factor activates the promoter of the CDK inhibitor p21W4F1/CIP1. The minimal region of the p21 promoter required for the NGF-induction was mapped to a contiguous stretch of 10 bp located 83 bases upstream of the transcription initiation site. This GC-rich region was shown to interact specifically with the transcription factor Sp1 and the related protein Sp3, in either exponentially-growing or NGF-treated PC12 cells. The addition of NGF resulted in an accumulation of the transcriptional co-activator p300 in complexes associated with the NGF-responsive region. Transcriptional activity of Sp1, Sp3 and p300 was specifically induced by NGF in a Gal4-fusion assay, indicating that induction of p21 during neuronal differentiation may involve regulation of the activity of these factors by NGF. Furthermore, p300 was able to act as a co-activator for Sp1-mediated transcriptional activation in PC12 cells, suggesting that p300 and Sp1 may cooperate in activating p21 transcription during the withdrawal of neuronal precursors from the cell cycle. This hypothesis is supported by experiments showing that p300 and Sp1 form complexes in PC12 cells.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

May 6, 1999

Volume

18

Issue

18

Start / End Page

2872 / 2882

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Sp3 Transcription Factor
  • Sp1 Transcription Factor
  • Recombinant Fusion Proteins
  • Rats
  • Promoter Regions, Genetic
  • PC12 Cells
  • Oncology & Carcinogenesis
 

Citation

APA
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MLA
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Billon, N., Carlisi, D., Datto, M. B., van Grunsven, L. A., Watt, A., Wang, X. F., & Rudkin, B. B. (1999). Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation. Oncogene, 18(18), 2872–2882. https://doi.org/10.1038/sj.onc.1202712
Billon, N., D. Carlisi, M. B. Datto, L. A. van Grunsven, A. Watt, X. F. Wang, and B. B. Rudkin. “Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation.Oncogene 18, no. 18 (May 6, 1999): 2872–82. https://doi.org/10.1038/sj.onc.1202712.
Billon N, Carlisi D, Datto MB, van Grunsven LA, Watt A, Wang XF, et al. Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation. Oncogene. 1999 May 6;18(18):2872–82.
Billon, N., et al. “Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation.Oncogene, vol. 18, no. 18, May 1999, pp. 2872–82. Pubmed, doi:10.1038/sj.onc.1202712.
Billon N, Carlisi D, Datto MB, van Grunsven LA, Watt A, Wang XF, Rudkin BB. Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation. Oncogene. 1999 May 6;18(18):2872–2882.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

May 6, 1999

Volume

18

Issue

18

Start / End Page

2872 / 2882

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Sp3 Transcription Factor
  • Sp1 Transcription Factor
  • Recombinant Fusion Proteins
  • Rats
  • Promoter Regions, Genetic
  • PC12 Cells
  • Oncology & Carcinogenesis