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Immunoglobulin prevents complement-mediated hyperacute rejection in swine-to-primate xenotransplantation.

Publication ,  Journal Article
Magee, JC; Collins, BH; Harland, RC; Lindman, BJ; Bollinger, RR; Frank, MM; Platt, JL
Published in: J Clin Invest
November 1995

Immunoglobulins regulate the complement system by activating complement on foreign surfaces and diverting reactive complement proteins away from autologous cell surfaces. Based on this model, we explored the ability of Ig to balance complement activation versus control in a pig-to-primate cardiac xenotransplantation model in which the binding of xenoreactive antibodies of the recipient to graft blood vessels and the activation of complement cause hyperacute rejection. Human IgG added to human serum caused a dose-dependent decrease in deposition of iC3b, cytotoxicity, and heparan sulfate release when the serum was incubated with porcine endothelial cells. This decrease was not caused by alteration in antibody binding or consumption of complement but presumably reflected decreased formation of C3 convertase on the endothelial cells. Infusion of purified human IgG into nonhuman primates prevented hyperacute rejection of porcine hearts transplanted into the primates. As expected, the transplants contained deposits of recipient Ig and C1q but not other complement components. The inhibition of complement on endothelial cell surfaces and in the xenotransplantation model supports the idea that IgG regulates the classical complement pathway and supports therapeutic use of that agent in humoral-mediated disease.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

November 1995

Volume

96

Issue

5

Start / End Page

2404 / 2412

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Swine
  • Primates
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
  • Humans
  • Heart Transplantation
  • Graft Rejection
  • Endothelium, Vascular
 

Citation

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Chicago
ICMJE
MLA
NLM
Magee, J. C., Collins, B. H., Harland, R. C., Lindman, B. J., Bollinger, R. R., Frank, M. M., & Platt, J. L. (1995). Immunoglobulin prevents complement-mediated hyperacute rejection in swine-to-primate xenotransplantation. J Clin Invest, 96(5), 2404–2412. https://doi.org/10.1172/JCI118297
Magee, J. C., B. H. Collins, R. C. Harland, B. J. Lindman, R. R. Bollinger, M. M. Frank, and J. L. Platt. “Immunoglobulin prevents complement-mediated hyperacute rejection in swine-to-primate xenotransplantation.J Clin Invest 96, no. 5 (November 1995): 2404–12. https://doi.org/10.1172/JCI118297.
Magee JC, Collins BH, Harland RC, Lindman BJ, Bollinger RR, Frank MM, et al. Immunoglobulin prevents complement-mediated hyperacute rejection in swine-to-primate xenotransplantation. J Clin Invest. 1995 Nov;96(5):2404–12.
Magee, J. C., et al. “Immunoglobulin prevents complement-mediated hyperacute rejection in swine-to-primate xenotransplantation.J Clin Invest, vol. 96, no. 5, Nov. 1995, pp. 2404–12. Pubmed, doi:10.1172/JCI118297.
Magee JC, Collins BH, Harland RC, Lindman BJ, Bollinger RR, Frank MM, Platt JL. Immunoglobulin prevents complement-mediated hyperacute rejection in swine-to-primate xenotransplantation. J Clin Invest. 1995 Nov;96(5):2404–2412.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

November 1995

Volume

96

Issue

5

Start / End Page

2404 / 2412

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Swine
  • Primates
  • Immunology
  • Immunoglobulin M
  • Immunoglobulin G
  • Humans
  • Heart Transplantation
  • Graft Rejection
  • Endothelium, Vascular