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Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc.

Publication ,  Journal Article
Friedman, HS; Burger, PC; Bigner, SH; Trojanowski, JQ; Brodeur, GM; He, XM; Wikstrand, CJ; Kurtzberg, J; Berens, ME; Halperin, EC
Published in: Am J Pathol
March 1988

D341 Med is a new continuous cell line and transplantable xenograft derived from a cerebellar medulloblastoma. This line grew in vitro in suspension culture with spontaneous macroscopic spheroid formation and demonstrated 20-fold amplification of c-myc. Cultured D341 Med cells injected subcutaneously into athymic mice grew as markedly cellular, highly invasive undifferentiated neoplasms. Intracranial tumors grew as markedly cellular mitotically active neoplasms largely located within the subarachnoid space or lining the ventricular system. Immunocytochemical analysis of the cell line and SQ tumors revealed the high (NFP-H) and middle (NFP-M) molecular weight (Mr) neurofilament proteins (NFPs). Immunoblots demonstrated the presence of molecular species that co-migrated with authentic human NFP-H and NFP-M. This cell line and transplantable xenograft may allow, in conjunction with the authors' other models of human medulloblastoma, analysis of the heterogeneous biologic properties and therapeutic sensitivity of this tumor.

Duke Scholars

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

March 1988

Volume

130

Issue

3

Start / End Page

472 / 484

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Radioimmunoassay
  • Phenotype
  • Pathology
  • Oncogenes
  • Neoplasm Transplantation
  • Microscopy, Electron
  • Mice, Nude
  • Mice
  • Medulloblastoma
 

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Friedman, H. S., Burger, P. C., Bigner, S. H., Trojanowski, J. Q., Brodeur, G. M., He, X. M., … Halperin, E. C. (1988). Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am J Pathol, 130(3), 472–484.
Friedman, H. S., P. C. Burger, S. H. Bigner, J. Q. Trojanowski, G. M. Brodeur, X. M. He, C. J. Wikstrand, J. Kurtzberg, M. E. Berens, and E. C. Halperin. “Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc.Am J Pathol 130, no. 3 (March 1988): 472–84.
Friedman HS, Burger PC, Bigner SH, Trojanowski JQ, Brodeur GM, He XM, et al. Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am J Pathol. 1988 Mar;130(3):472–84.
Friedman HS, Burger PC, Bigner SH, Trojanowski JQ, Brodeur GM, He XM, Wikstrand CJ, Kurtzberg J, Berens ME, Halperin EC. Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am J Pathol. 1988 Mar;130(3):472–484.
Journal cover image

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

March 1988

Volume

130

Issue

3

Start / End Page

472 / 484

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Radioimmunoassay
  • Phenotype
  • Pathology
  • Oncogenes
  • Neoplasm Transplantation
  • Microscopy, Electron
  • Mice, Nude
  • Mice
  • Medulloblastoma