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Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells.

Publication ,  Journal Article
Rusche, JR; Lynn, DL; Robert-Guroff, M; Langlois, AJ; Lyerly, HK; Carson, H; Krohn, K; Ranki, A; Gallo, RC; Bolognesi, DP
Published in: Proc Natl Acad Sci U S A
October 1987

The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1987

Volume

84

Issue

19

Start / End Page

6924 / 6928

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Recombinant Proteins
  • Moths
  • Insect Viruses
  • HIV
  • Goats
  • Genetic Vectors
  • Genes, Viral
  • Genes
  • Cell Line
 

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Rusche, J. R., Lynn, D. L., Robert-Guroff, M., Langlois, A. J., Lyerly, H. K., Carson, H., … Bolognesi, D. P. (1987). Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells. Proc Natl Acad Sci U S A, 84(19), 6924–6928. https://doi.org/10.1073/pnas.84.19.6924
Rusche, J. R., D. L. Lynn, M. Robert-Guroff, A. J. Langlois, H. K. Lyerly, H. Carson, K. Krohn, A. Ranki, R. C. Gallo, and D. P. Bolognesi. “Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells.Proc Natl Acad Sci U S A 84, no. 19 (October 1987): 6924–28. https://doi.org/10.1073/pnas.84.19.6924.
Rusche JR, Lynn DL, Robert-Guroff M, Langlois AJ, Lyerly HK, Carson H, et al. Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6924–8.
Rusche, J. R., et al. “Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells.Proc Natl Acad Sci U S A, vol. 84, no. 19, Oct. 1987, pp. 6924–28. Pubmed, doi:10.1073/pnas.84.19.6924.
Rusche JR, Lynn DL, Robert-Guroff M, Langlois AJ, Lyerly HK, Carson H, Krohn K, Ranki A, Gallo RC, Bolognesi DP. Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6924–6928.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1987

Volume

84

Issue

19

Start / End Page

6924 / 6928

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Recombinant Proteins
  • Moths
  • Insect Viruses
  • HIV
  • Goats
  • Genetic Vectors
  • Genes, Viral
  • Genes
  • Cell Line